Results 41 to 50 of about 2,993 (171)

Aequorin-based measurements of intracellular Ca²⁺-signatures in plant cells

Biological Procedures Online, 2002
Due to the involvement of calcium as a main second messenger in the plant signaling pathway, increasing interest has been focused on the calcium signatures supposed to be involved in the patterning of the specific response associated to a given stimulus.
Mithöfer Axel, Mazars Christian
doaj   +1 more source

Two types of GLR channels cooperate differently in light and dark growth of Arabidopsis seedlings

BMC Plant Biology, 2023
Background GLutamate Receptor-like (GLR) channels are multimeric, ionotropic, ligand-gated plant transmembrane receptors. They are homologous to mammalian glutamate receptors, iGLuRs, which are critical to neuronal function.
Weronika Krzeszowiec, Aleksandra Lewandowska, Jan Jakub Lyczakowski, Kateryna Bebko, Sandra S. Scholz, Halina Gabryś   +5 more
doaj   +1 more source

Imaging of Molecular and Developmental Responses to Abiotic Stresses in Reproductive Tissues

Physiologia Plantarum, Volume 178, Issue 1, January/February 2026.
ABSTRACT Abiotic stresses, such as drought, salinity, and extreme temperatures, have profound effects on plant reproduction, often leading to reduced fertility and yield. Reproduction in plants involves complex interactions between diverse cells, necessitating spatiotemporal resolution to understand how stress impacts each component of this intricate ...
Hana Daryanavard, Teresa Paraiso, María Cielo Pasten, Bianca Maria Orlando Marchesano, Marta Adelina Mendes, Hélène S. Robert, Francesca Resentini, Joëlle K. Mühlemann   +7 more
wiley   +1 more source

Cloning and expression of aequorin photoprotein using intein tag

Iranian South Medical Journal, 2015
Background: Intein (INT), is the internal parts of the protein which can be separated from the immature protein during protein splicing process. This sequence requires no specific enzyme or cofactor for separation.
Elah sadat Seyed Hosseini, Mehdi Zeinoddini, Zeinoddini Fallah, Alireza Saeedinia   +3 more
doaj  

Chitin Soil Amendment Triggers Systemic Plant Disease Resistance Through Enhanced Pattern‐Triggered Immunity

Plant Biotechnology Journal, Volume 23, Issue 11, Page 5032-5044, November 2025.
ABSTRACT Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin‐induced systemic effects in plants remain unknown. In this study, we investigated the effects of soil amendment with crab chitin flakes (hereafter chitin)
Moffat Makechemu, Yukihisa Goto, Marc W. Schmid, Helen Zbinden, Victoria Widrig, Moritz Kaufmann, Joël F. Pothier, Beat Keller, Cyril Zipfel   +8 more
wiley   +1 more source

Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae

Hayati Journal of Biosciences, 2010
Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited
HERMANSYAH
doaj   +3 more sources

The role of His 16, Tyr 82 and Trp 86 in color shift, activity and thermostability of aequorin photoprotein

Iranian South Medical Journal, 2015
Background: Aequorin is a calcium sensitive photoprotein composed of apoaequorin (189 amino acid), oxygen and coelenterazine (imidazopyrazine chromophore). Activity of this photoprotein in calcium presence is blue photon emission (469 nm).
Faeazeh Ghanbartalab, Mehdi Zeinoddini, Saman Hosseinkhani, Masoome Esmailinejad, sayedealah Hosseini, Masoome Laali   +5 more
doaj  

Pretreatment with apoaequorin protects hippocampal CA1 neurons from oxygen-glucose deprivation.

PLoS ONE, 2013
Ischemic stroke affects ∼795,000 people each year in the U.S., which results in an estimated annual cost of $73.7 billion. Calcium is pivotal in a variety of neuronal signaling cascades, however, during ischemia, excess calcium influx can trigger ...
Julia A Detert, Erin L Adams, Jacob D Lescher, Jeri-Anne Lyons, James R Moyer   +4 more
doaj   +1 more source

Non-invasive in vivo imaging of calcium signaling in mice. [PDF]

PLoS ONE, 2007
Rapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)])
Kelly L Rogers, Sandrine Picaud, Emilie Roncali, Raphaël Boisgard, Cesare Colasante, Jacques Stinnakre, Bertrand Tavitian, Philippe Brûlet   +7 more
doaj   +1 more source

TRPM8 levels determine tumor vulnerability to channel agonists

Molecular Oncology, Volume 19, Issue 10, Page 2905-2920, October 2025.
TRPM8 is a Ca2+ permissive channel. Regardless of the amount of its transcript, high levels of TRPM8 protein mark different tumors, including prostate, breast, colorectal, and lung carcinomas. Targeting TRPM8 with channel agonists stimulates inward calcium currents followed by emptying of cytosolic Ca2+ stores in cancer cells.
Alessandro Alaimo, Francesco Giuseppe Carbone, Kristi Buzo, Nicole Annesi, Sacha Genovesi, Annalisa Lorenzato, Karen Widmann, Michela Libergoli, Elisa Marmocchi, Giovanni Bertalot, Alberto Brolese, Mauro Giulio Papotti, Luca Molinaro, Orazio Caffo, Mattia Barbareschi, Alberto Bardelli, Alessandro Romanel, Sabrina Arena, Andrea Lunardi   +18 more
wiley   +1 more source