Results 51 to 60 of about 2,993 (171)

A new short-term toxicity assay using Aspergillus awamori with recombinant aequorin gene

open access: yesBMC Microbiology, 2005
Background Most currently available short-term toxicity assays are based on bacterial cells. Therefore there is a need for novel eukaryotic microbial bioassays that will be relevant to higher eukaryotes such as animals and plants.
Zwinderman Mark   +2 more
doaj   +1 more source

Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato

open access: yesHorticulturae
The tomato (Solanum lycopersicum L.), a widely cultivated and economically important vegetable crop, is subject to a number of biotic and abiotic stresses in nature.
Lulu Liu   +3 more
doaj   +1 more source

Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti

open access: yesBMC Microbiology, 2009
Background During the interaction between rhizobia and leguminous plants the two partners engage in a molecular conversation that leads to reciprocal recognition and ensures the beginning of a successful symbiotic integration.
Mariani Paola   +4 more
doaj   +1 more source

Electrochemical Analysis Reveals the Chemiluminescence Processes of Coelenterazine to Deprotonated Coelenteramide

open access: yesElectroanalysis, Volume 37, Issue 8, August 2025.
Coelenterazine is the most common imidazopyrazinone‐based luciferin in marine organisms. The electrochemical main peaks of coelenterazine are observed at approximately around 0.12 and 0.62 Vs in the cyclic voltammetry curve. Theoretical analysis suggests that coelenterazine is converted to the coelenteramide around 0.12 V, and the coelenteramide is ...
Yoshihiro Ohmiya   +4 more
wiley   +1 more source

Capsaicin Is a Negative Allosteric Modulator of the 5-HT3 Receptor

open access: yesFrontiers in Pharmacology, 2020
In this study, effects of capsaicin, an active ingredient of the capsicum plant, were investigated on human 5-hydroxytryptamine type 3 (5-HT3) receptors.
Eslam El Nebrisi   +9 more
doaj   +1 more source

Different NaCl-induced calcium signatures in the Arabidopsis thaliana ecotypes Col-0 and C24.

open access: yesPLoS ONE, 2015
A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity ...
Sandra M Schmöckel   +5 more
doaj   +1 more source

Comprehensive functional splicing analysis of non‐canonical CNGB3 variants using in vitro minigene splice assays

open access: yesThe Journal of Pathology, Volume 266, Issue 3, Page 322-336, July 2025.
Abstract Variants in the CNGB3 gene, encoding the B3‐subunit of the cone photoreceptor cyclic nucleotide gated channel, are a major cause of autosomal recessive achromatopsia, a rare inherited retinal disease. The mutation spectrum of achromatopsia‐associated CNGB3 variants comprises all types of mutations, including those that are straightforward to ...
Katharina Rawnsley   +3 more
wiley   +1 more source

Arabidopsis guard cell CO2/HCO3 − response mutant screening by an aequorin-based calcium imaging system

open access: yesPlant Methods, 2020
Background The increase in atmospheric CO2 is causing a number of changes in plant growth such as increases in leaf area and number, branching, plant size and biomass, and growth rate.
Mengmeng Tang   +8 more
doaj   +1 more source

Effects of Adalimumab on Mitochondria of Psoriatic Mesenchymal Stem Cells

open access: yesJournal of Cellular and Molecular Medicine, Volume 29, Issue 11, June 2025.
ABSTRACT Psoriasis is a systemic immune‐mediated disorder involving multiple signalling pathways. Recent attempts to treat psoriasis involve monoclonal antibodies that block different inflammatory pathways. The monoclonal antibody Adalimumab (ADM) is one of the biologics that block the inflammatory cascade of TNF‐alpha.
Mariangela Di Vincenzo   +7 more
wiley   +1 more source

Thein SituRegeneration and Extraction of Recombinant Aequorin fromEscherichia coliCells and the Purification of Extracted Aequorin

open access: yesProtein Expression and Purification, 1999
Recombinant apoaequorin expressed in the periplasmic space of Escherichia coli cells was regenerated into aequorin and extracted from the cells, simultaneously, using a buffer that contained coelenterazine. Due to the mild extraction conditions, the impurities in the extract were minimal.
O, Shimomura, S, Inouye
openaire   +2 more sources

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