Results 51 to 60 of about 3,245 (169)

Pretreatment with apoaequorin protects hippocampal CA1 neurons from oxygen-glucose deprivation.

open access: yesPLoS ONE, 2013
Ischemic stroke affects ∼795,000 people each year in the U.S., which results in an estimated annual cost of $73.7 billion. Calcium is pivotal in a variety of neuronal signaling cascades, however, during ischemia, excess calcium influx can trigger ...
Julia A Detert   +4 more
doaj   +1 more source

Non-invasive in vivo imaging of calcium signaling in mice. [PDF]

open access: yesPLoS ONE, 2007
Rapid and transient elevations of Ca(2+) within cellular microdomains play a critical role in the regulation of many signal transduction pathways. Described here is a genetic approach for non-invasive detection of localized Ca(2+) concentration ([Ca(2+)])
Kelly L Rogers   +7 more
doaj   +1 more source

Thein SituRegeneration and Extraction of Recombinant Aequorin fromEscherichia coliCells and the Purification of Extracted Aequorin

open access: yesProtein Expression and Purification, 1999
Recombinant apoaequorin expressed in the periplasmic space of Escherichia coli cells was regenerated into aequorin and extracted from the cells, simultaneously, using a buffer that contained coelenterazine. Due to the mild extraction conditions, the impurities in the extract were minimal.
O, Shimomura, S, Inouye
openaire   +2 more sources

Calcium signaling in crops

open access: yesNew Phytologist, Volume 249, Issue 4, Page 1644-1658, February 2026.
Summary Calcium (Ca2+) signaling is integral to nearly all aspects of plant biology, including development and responses to biotic and abiotic stresses. It operates through two main layers: the generation of Ca2+ signals and their decoding by Ca2+‐binding proteins, which act early in diverse signaling pathways. The system exhibits remarkable robustness
Chunxia Zhang, Yang Song, Jörg Kudla
wiley   +1 more source

A new short-term toxicity assay using Aspergillus awamori with recombinant aequorin gene

open access: yesBMC Microbiology, 2005
Background Most currently available short-term toxicity assays are based on bacterial cells. Therefore there is a need for novel eukaryotic microbial bioassays that will be relevant to higher eukaryotes such as animals and plants.
Zwinderman Mark   +2 more
doaj   +1 more source

Imaging of Molecular and Developmental Responses to Abiotic Stresses in Reproductive Tissues

open access: yesPhysiologia Plantarum, Volume 178, Issue 1, January/February 2026.
ABSTRACT Abiotic stresses, such as drought, salinity, and extreme temperatures, have profound effects on plant reproduction, often leading to reduced fertility and yield. Reproduction in plants involves complex interactions between diverse cells, necessitating spatiotemporal resolution to understand how stress impacts each component of this intricate ...
Hana Daryanavard   +7 more
wiley   +1 more source

Aequorin-Based In Vivo Luminescence Imaging Detects Calcium Signalling in Response to Biotic and Abiotic Stresses in Tomato

open access: yesHorticulturae
The tomato (Solanum lycopersicum L.), a widely cultivated and economically important vegetable crop, is subject to a number of biotic and abiotic stresses in nature.
Lulu Liu   +3 more
doaj   +1 more source

Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti

open access: yesBMC Microbiology, 2009
Background During the interaction between rhizobia and leguminous plants the two partners engage in a molecular conversation that leads to reciprocal recognition and ensures the beginning of a successful symbiotic integration.
Mariani Paola   +4 more
doaj   +1 more source

Chitin Soil Amendment Triggers Systemic Plant Disease Resistance Through Enhanced Pattern‐Triggered Immunity

open access: yesPlant Biotechnology Journal, Volume 23, Issue 11, Page 5032-5044, November 2025.
ABSTRACT Chitin triggers localised and systemic plant immune responses, making it a promising treatment for sustainable disease resistance. However, the precise molecular mechanisms underlying chitin‐induced systemic effects in plants remain unknown. In this study, we investigated the effects of soil amendment with crab chitin flakes (hereafter chitin)
Moffat Makechemu   +8 more
wiley   +1 more source

Capsaicin Is a Negative Allosteric Modulator of the 5-HT3 Receptor

open access: yesFrontiers in Pharmacology, 2020
In this study, effects of capsaicin, an active ingredient of the capsicum plant, were investigated on human 5-hydroxytryptamine type 3 (5-HT3) receptors.
Eslam El Nebrisi   +9 more
doaj   +1 more source

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