Results 61 to 70 of about 2,993 (171)
Streptavidin-Aequorin Fusion Protein for Bioluminescent Immunoassay [PDF]
Bioscience, Biotechnology, and Biochemistry, 2011 The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model analyte to test STA-AQ in an immunoassay.
INOUYE, Satoshi +3 more
openaire +2 more sources
Plants, 2020 The root tip responds to mechanical stimulation with a transient increase in cytosolic free calcium as a possible second messenger. Although the root tip will grow through a heterogeneous soil nutrient supply, little is known of the consequence of ...
Elsa Matthus +6 more
doaj +1 more source
New Phytologist, Volume 248, Issue 5, Page 2192-2197, December 2025.
Youzheng Ning +9 more
wiley +1 more source
Plant, Cell &Environment, Volume 48, Issue 6, Page 3939-3954, June 2025.ABSTRACT Calcium (Ca2+)‐dependent signalling plays a well‐characterised role in the perception and response mechanisms to environmental stimuli in plant cells. In the context of a constantly changing environment, it is fundamental to understand how crop yield and microalgal biomass productivity are affected by external factors. Ca2+ signalling is known
Matteo Pivato +3 more
wiley +1 more source
Expression and Characterization of i-Photina as an Efficient Photoprotein
Iranian South Medical Journal, 2015 Background: Nowadays photoproteins are excellent reporter systems as they have virtually no background. The most commonly studied photoprotein is aequorin. But because of it’s low quantum yield and very fast reaction kinetic this photoprotein assay
Fatemeh Ahmadi +2 more
doaj
PLoS ONE, 2015 Aspergillus fumigatus is an inhaled fungal pathogen of human lungs, the developmental growth of which is reliant upon Ca2+-mediated signalling. Ca2+ signalling has regulatory significance in all eukaryotic cells but how A.
Alberto Muñoz +5 more
doaj +1 more source
New Phytologist, Volume 246, Issue 3, Page 1236-1255, May 2025.Summary Lipids play crucial roles in plant–microbe interactions, functioning as structural components, signaling molecules, and microbe‐associated molecular patterns (MAMPs). However, the mechanisms underlying lipid perception and signaling in plants remain largely unknown. Here, we investigate the immune responses activated in barley (Hordeum vulgare)
Pia Saake +11 more
wiley +1 more source
Titration of Recombinant Aequorin with Calcium Chloride
Biochemical and Biophysical Research Communications, 1996 The photoprotein aequorin emits light in the presence of a trace of Ca2+. The primary structure of the protein indicates the presence of three Ca2+-binding sites, whereas the luminometric titration of heterogeneous natural aequorin with Ca2+ has shown that the light emission takes place by the binding of two Ca2+ ions.
O, Shimomura, S, Inouye
openaire +2 more sources
Biomolecules, 2020 The main events in chaperone-assisted protein folding are the binding and ligand-induced release of substrate proteins. Here, we studied the location of denatured proteins previously bound to the GroEL chaperonin resulting from the action of the GroES co-
Victor Marchenkov +4 more
doaj +1 more source
Extracellular ATP: an emerging multifaceted regulator of plant fitness
Plant Biotechnology Journal, Volume 23, Issue 5, Page 1771-1782, May 2025.Summary Adenosine 5′‐triphosphate (ATP) is the energy currency of living organisms and the primary form of organic phosphate (Po) involved in cellular metabolism. In plants, some ATP is released into the extracellular matrix (ECM) in response to various stimuli, where it functions as extracellular ATP (eATP), a key signalling molecule.
De Peng Yuan, Daewon Kim, Yuan Hu Xuan
wiley +1 more source