Results 11 to 20 of about 5,761 (165)
Mutational optimization of the coelenterazine-dependent luciferase from
Plant Methods, 2008 Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated.
Woo Jongchan, von Arnim Albrecht G
doaj +3 more sources
Tumor-targeting bioluminescent bacteria for in vivo imaging [PDF]
Engineering MicrobiologyAs the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy.
Chenghao Ma +6 more
doaj +2 more sources
AB-MDMSBA-Not a Synthetic Cannabinoid Receptor Agonist. [PDF]
Pharmacol Res PerspectThis study uses classical in vitro pharmacology approaches to establish that AB‐MDMSBA does not bind to or activate the cannabinoid CB1 receptor and therefore should not be classified as a synthetic cannabinoid receptor agonist. ABSTRACT Synthetic cannabinoids remain one of the most prominent classes of New Psychoactive Substances (NPS).
Patel M +4 more
europepmc +2 more sources
A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis. [PDF]
PLoS ONEBioluminescence is the production of visible light by living organisms thanks to a chemical reaction, implying the oxidation of a substrate called luciferin catalyzed by an enzyme, the luciferase.
Constance Coubris +3 more
doaj +2 more sources
Investigation of the Superoxide Anion-Triggered Chemiluminescence of Coelenterazine Analogs
Applied Sciences, 2023 Reactive oxygen species (ROS), including superoxide anion, are involved in regulating various signaling pathways and are also responsible for oxidative stress. Sensing superoxide anion is of particular importance due to its biological significance.
El Hadi Erbiai +3 more
doaj +1 more source
PLoS ONE, 2021 The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation of apoAequorin with coelenterazine and molecular oxygen (O2).
Satoshi Inouye +5 more
doaj +1 more source
Improved Messenger RNA Stability and Biocompatibility Through Self-Gelatinizable Nucleic Acids. [PDF]
Biotechnol BioengABSTRACT Recent advances in the chemical synthesis and modification of messenger RNA (mRNA) have generated growing interest in mRNA‐based therapeutics. However, the inherent instability of mRNA in vivo and during storage remains a major challenge, requiring the development of safe and effective delivery systems.
Tanifuji T +5 more
europepmc +2 more sources
Evidence for de novo Biosynthesis of the Luminous Substrate Coelenterazine in Ctenophores
iScience, 2020 Summary: Coelenterazine is a key substrate involved in marine bioluminescence which is used for light-production by at least nine phyla. Some luminous animals, such as the hydromedusa Aequorea, lack the ability to produce coelenterazine endogenously and ...
Manabu Bessho-Uehara +5 more
doaj +1 more source
Chemosensors, 2022 Superoxide anion is a reactive oxygen species (ROS) of biological interest. More specifically, it plays a role in intra- and intercellular signaling, besides being associated with conditions such as inflammation and cancer.
José Pedro Silva +3 more
doaj +1 more source
Occurrence of Isopenicillin-N-Synthase Homologs in Bioluminescent Ctenophores and Implications for Coelenterazine Biosynthesis. [PDF]
PLoS ONE, 2015 The biosynthesis of the luciferin coelenterazine has remained a mystery for decades. While not all organisms that use coelenterazine appear to make it themselves, it is thought that ctenophores are a likely producer.
Warren R Francis +4 more
doaj +1 more source