Investigation of the Superoxide Anion-Triggered Chemiluminescence of Coelenterazine Analogs [PDF]
Applied Sciences, 2023 Reactive oxygen species (ROS), including superoxide anion, are involved in regulating various signaling pathways and are also responsible for oxidative stress. Sensing superoxide anion is of particular importance due to its biological significance.
El Hadi Erbiai +3 more
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PLoS ONE, 2021 The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation of apoAequorin with coelenterazine and molecular oxygen (O2).
Satoshi Inouye +5 more
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Evidence for de novo Biosynthesis of the Luminous Substrate Coelenterazine in Ctenophores
iScience, 2020 Summary: Coelenterazine is a key substrate involved in marine bioluminescence which is used for light-production by at least nine phyla. Some luminous animals, such as the hydromedusa Aequorea, lack the ability to produce coelenterazine endogenously and ...
Manabu Bessho-Uehara +5 more
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Coelenterazine sulfotransferase from Renilla muelleri.
PLoS ONE, 2022 The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence ...
George Tzertzinis +7 more
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Occurrence of Isopenicillin-N-Synthase Homologs in Bioluminescent Ctenophores and Implications for Coelenterazine Biosynthesis. [PDF]
PLoS ONE, 2015 The biosynthesis of the luciferin coelenterazine has remained a mystery for decades. While not all organisms that use coelenterazine appear to make it themselves, it is thought that ctenophores are a likely producer.
Warren R Francis +4 more
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Development of a Coelenterazine Derivative with Enhanced Superoxide Anion-Triggered Chemiluminescence in Aqueous Solution [PDF]
Chemosensors, 2022 Superoxide anion is a reactive oxygen species (ROS) of biological interest. More specifically, it plays a role in intra- and intercellular signaling, besides being associated with conditions such as inflammation and cancer.
José Pedro Silva +3 more
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Oxygen Activation of Apo‐obelin–Coelenterazine Complex
ChemBioChem, 2013 AbstractCa2+‐regulated photoproteins use a noncovalently bound 2‐hydroperoxycoelenterazine ligand to emit light in response to Ca2+ binding. To better understand the mechanism of formation of active photoprotein from apoprotein, coelenterazine and molecular oxygen, we investigated the spectral properties of the anaerobic apo‐obelin–coelenterazine ...
Eremeeva, E. +6 more
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The key residue responsible for the red shift of bioluminescence spectra of light-sensitive Ca2+-regulated photoproteins of ctenophores [PDF]
Scientific ReportsIsoforms of some ctenophore photoproteins show different maxima of bioluminescence spectra— the ones of mnemiopsin and bolinopsin have λmax at 490 and 500 nm, while λmax for velamin isoforms are at 500 and 508 nm.
Ludmila P. Burakova, Eugene S. Vysotski
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A coelenterazine-type bioluminescent probe for nitroreductase imaging
Organic & Biomolecular Chemistry, 2018 Novel coelenterazine-type bioluminescent probes have been designed and synthesized to detect nitroreductase (NTR) in hypoxic tumors.
Xingye Yang +4 more
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Mutational optimization of the coelenterazine-dependent luciferase from
Plant Methods, 2008 Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated.
Woo Jongchan, von Arnim Albrecht G
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