Results 21 to 30 of about 6,471 (200)

Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins

Sensors, 2023
Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine
Sung-Bae Kim, Genta Kamiya, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki   +4 more
doaj   +1 more source

Target-Oriented Synthesis of Marine Coelenterazine Derivatives with Anticancer Activity by Applying the Heavy-Atom Effect

Biomedicines, 2021
Photodynamic therapy (PDT) is an anticancer therapeutic modality with remarkable advantages over more conventional approaches. However, PDT is greatly limited by its dependence on external light sources.
Carla M. Magalhães, Patricia González-Berdullas, Diana Duarte, Ana Salomé Correia, José E. Rodríguez-Borges, Nuno Vale, Joaquim C. G. Esteves da Silva, Luís Pinto da Silva   +7 more
doaj   +1 more source

Cardioluminescence in Transgenic Zebrafish Larvae: A Calcium Imaging Tool to Study Drug Effects and Pathological Modeling

Biomedicines, 2021
Zebrafish embryos and larvae have emerged as an excellent model in cardiovascular research and are amenable to live imaging with genetically encoded biosensors to study cardiac cell behaviours, including calcium dynamics. To monitor calcium ion levels in
Manuel Vicente, Jussep Salgado-Almario, Michelle M. Collins, Antonio Martínez-Sielva, Masafumi Minoshima, Kazuya Kikuchi, Beatriz Domingo, Juan Llopis   +7 more
doaj   +1 more source

Structures of coelenterazine and coelenterazine sulfate and their interconversion.

, 2022
Structures of coelenterazine and coelenterazine sulfate and their interconversion.
Ira Schildkraut (29742), Ivan R. Corrêa Jr. (11083985), Laurence Ettwiller (15718), George Tzertzinis (154499), Jack Benner (9571), Elizabeth Brown (281332), Colleen McClung (3402752), Brenda Baker (174368)   +7 more
core   +1 more source

Localization of the Catalytic Domain of Copepod Luciferases: Analysis of Truncated Mutants of the Metridia longa Luciferase

Life, 2023
Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova, Marina D. Larionova, Igor A. Korotov, Eugene S. Vysotski   +3 more
doaj   +1 more source

Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays

Sensors, 2023
In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki   +3 more
doaj   +1 more source

Theoretical Study of the Thermolysis Reaction and Chemiexcitation of Coelenterazine Dioxetanes [PDF]

, 2022
Coelenterazine and other imidazopyrazinones are important bioluminescent substrates widespread in marine species and can be found in eight phyla of luminescent organisms.
Joaquim C. G. Esteves da Silva (12633349), Joaquim C. G. Esteves da Silva, Carla M. Magalhães (12633346), Luís Pinto da Silva (1656373), Carla M. Magalhães, Luís Pinto da Silva   +5 more
core   +2 more sources

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate.

PLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye, Jun-Ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-Ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya   +7 more
doaj   +2 more sources

Scheme for coelenterazine sulfate chemical synthesis.

, 2022
Scheme for coelenterazine sulfate chemical synthesis.
Ira Schildkraut (29742), Ivan R. Corrêa Jr. (11083985), Laurence Ettwiller (15718), George Tzertzinis (154499), Jack Benner (9571), Elizabeth Brown (281332), Colleen McClung (3402752), Brenda Baker (174368)   +7 more
core   +1 more source

Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging. [PDF]

PLoS ONE, 2016
The increased importance of in vivo diagnostics has posed new demands for imaging technologies. In that regard, there is a need for imaging molecules capable of expanding the applications of current state-of-the-art imaging in vivo diagnostics.
Kristen M Grinstead, Laura Rowe, Charles M Ensor, Smita Joel, Pirouz Daftarian, Emre Dikici, Jean-Marc Zingg, Sylvia Daunert   +7 more
doaj   +1 more source