Results 21 to 30 of about 7,015 (221)

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate.

PLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye, Jun-Ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-Ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya   +7 more
doaj   +3 more sources

Oxygen Activation of Apo‐obelin–Coelenterazine Complex

ChemBioChem, 2013
AbstractCa2+‐regulated photoproteins use a noncovalently bound 2‐hydroperoxycoelenterazine ligand to emit light in response to Ca2+ binding. To better understand the mechanism of formation of active photoprotein from apoprotein, coelenterazine and molecular oxygen, we investigated the spectral properties of the anaerobic apo‐obelin–coelenterazine ...
Eremeeva, E., Natashin, P.V., Song, L., Zhou, Y., van Berkel, W.J.H., Liu, Z.J., Vysotski, E.S.   +6 more
openaire   +4 more sources

Comparative investigation into the anticancer activity of analogs of marine coelenterazine and coelenteramine. [PDF]

Bioorganic chemistry (Print)
Cancer is still one of the most challenging diseases to treat, making the pursuit for novel molecules with potential anticancer activity an important research topic.
Carla M. Magalhães, R. Pereira, El Hadi Erbiai, Patricia González-Berdullas, J. E. da Silva, David M. Pereira, Luís Pinto da Silva   +6 more
semanticscholar   +2 more sources

C-Series Coelenterazine-Driven Bioluminescence Signature Imaging

International Journal of Molecular Sciences, 2022
The present study introduces a unique BL signature imaging system with novel CTZ analogues named “C-series.” Nine kinds of C-series CTZ analogues were first synthesized, and BL intensity patterns and spectra were then examined according to the marine ...
Genta Kamiya, Nobuo Kitada, T. Furuta, T. Hirano, S. Maki, Sung Bae Kim   +5 more
semanticscholar   +3 more sources

Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes

International Journal of Molecular Sciences, 2023
Bioluminescence-based probes have long been used to quantify and visualize biological processes in vitro and in vivo. Over the past years, we have witnessed the trend of bioluminescence-driven optogenetic systems.
Tianyu Jiang, Jingwen Song, Youming Zhang   +2 more
semanticscholar   +2 more sources

Evaluation of the Anticancer Activity and Chemiluminescence of a Halogenated Coelenterazine Analog [PDF]

Journal of Photochemistry and Photobiology A: Chemistry, 2022
Chemiluminescence is a remarkable process in which light is emitted due to a chemical reaction, without the need for photoexcitation. Among some of the most well-known chemiluminescent systems is that of marine Coelenterazine.
José Pedro Silva, Patricia González-Berdullas, M. Pereira, D. Duarte, J. Rodríguez-Borges, N. Vale, J. E. D. Esteves da Silva, Luís Pinto da Silva   +7 more
semanticscholar   +2 more sources

Regiospecific Coelenterazine Analogs for Bioassays and Molecular Imaging.

Bioconjugate Chemistry
Bioluminescence (BL) generated by luciferase-coelenterazine (CTZ) reactions is broadly employed as an optical readout in bioassays and in vivo molecular imaging.
Genta Kamiya, Nobuo Kitada, Tadaomi Furuta, Suresh Thangudu, A. Natarajan, R. Paulmurugan, Sung-Bae Kim, Shojiro A. Maki   +7 more
semanticscholar   +4 more sources

A coelenterazine-type bioluminescent probe for nitroreductase imaging

Organic & Biomolecular Chemistry, 2018
Novel coelenterazine-type bioluminescent probes have been designed and synthesized to detect nitroreductase (NTR) in hypoxic tumors.
Xingye Yang, Zhenzhen Li, Tianyu Jiang, Lupei Du, Minyong Li   +4 more
openaire   +3 more sources

Investigation of the Chemiluminescent Reaction of a Fluorinated Analog of Marine Coelenterazine

Materials
Bioluminescence (BL) and chemiluminescence (CL) are remarkable processes in which light is emitted due to (bio)chemical reactions. These reactions have attracted significant attention for various applications, such as biosensing, bioimaging, and ...
Carla M. Magalhães, Joaquim C. G. Esteves da Silva, Luís Pinto da Silva   +2 more
semanticscholar   +4 more sources

Mutational optimization of the coelenterazine-dependent luciferase from Renilla [PDF]

Plant Methods, 2008
Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated.
Woo Jongchan, von Arnim Albrecht G
doaj   +3 more sources