Results 1 to 10 of about 6,926,142 (285)

Emerging tools for bioluminescence imaging

Current Opinion in Chemical Biology, 2021
Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become a widely used technique to interrogate gene expression and cell fate, both in small and large animal models of research.
Laura Mezzanotte
exaly   +4 more sources

Semisynthetic, Multicolor Probes for Bioluminescence Imaging [PDF]

ACS Omega
Bioluminescence imaging is a sensitive and noninvasive technique for monitoring molecular and cellular processes in living systems. NanoLuc is one of the brightest and most widely used luciferase enzymes, however, the native blue emission of NanoLuc limits its applications due to poor sample penetration.
Suong Tran, Kat Usavage, Lila P. Halbers, Claire Choplick, Grace Helton, Brenden Young, Jennifer A. Prescher, Colin M. Rathbun   +7 more
doaj   +4 more sources

NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing

Nature Communications, 2020
Conventional bioluminescence imaging usually operates in the visible region and its performance is limited by strong tissue absorption and scattering. Here, the authors present bioluminescence probes (BPs) with emission in the second near infrared (NIR ...
Lingfei Lu, Benhao Li, Suwan Ding, Yong Fan, Shangfeng Wang, Caixia Sun, Mengyao Zhao, Chun-Xia Zhao, Fan Zhang   +8 more
doaj   +3 more sources

Quantitative Assessment on Optical Properties as a Basis for Bioluminescence Imaging: An Experimental and Numerical Approach to the Transport of Optical Photons in Phantom Materials [PDF]

Sensors, 2023
Bioluminescence imaging (BLI) is a widely used technique in preclinical scientific research, particularly in the development of mRNA-based medications and non-invasive tumor diagnostics. It has become an essential tool in current science.
Tim Karstens, Theresa Staufer, Rasmus Buchin, Florian Grüner   +3 more
doaj   +2 more sources

Qualitative in vivo Bioluminescence Imaging

Bio-Protocol, 2018
Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in ...
Devbarna Sinha, Zalitha Pieterse, Pritinder Kaur   +2 more
doaj   +5 more sources

Enhancing Bioluminescence Imaging of Cultured Tissue Explants Using Optical Telecompression [PDF]

Sensors
Long-term observation of single-cell oscillations within tissue networks is now possible by combining bioluminescence reporters with stable tissue explant culture techniques.
Jihwan Myung
doaj   +2 more sources

A practical guideline for in vivo bioluminescence imaging. [PDF]

Mol Cells
Bioluminescence imaging (BLI) using luciferase-expressing cancer cells is a widely adopted method for non-invasive, real-time monitoring of tumor growth, metastasis, and therapeutic responses in vivo. Here, we summarize key considerations for effective implementation of BLI, including luciferin administration and data quantification.
Lee H, Chae JS, Park HW.
europepmc   +3 more sources

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes

Molecular Therapy: Nucleic Acids, 2013
Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Casey A Maguire, M Sarah Bovenberg, Matheus HW Crommentuijn, Johanna M Niers, Mariam Kerami, Jian Teng, Miguel Sena-Esteves, Christian E Badr, Bakhos A Tannous   +8 more
doaj   +3 more sources

Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins. [PDF]

Chembiochem, 2021
AbstractBioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues.
Brennan CK, Ornelas MY, Yao ZW, Prescher JA.   +3 more
europepmc   +5 more sources

Noninvasive Bioluminescence Imaging in Small Animals [PDF]

ILAR Journal, 2008
There has been a rapid growth of bioluminescence imaging applications in small animal models in recent years, propelled by the availability of instruments, analysis software, reagents, and creative approaches to apply the technology in molecular imaging. Advantages include the sensitivity of the technique as well as its efficiency, relatively low cost,
Kurt Zinn, Stuart Frank
exaly   +3 more sources