Emerging tools for bioluminescence imaging [PDF]
Current Opinion in Chemical Biology, 2021 Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate conventionally named luciferin, and various cofactors. BL imaging has become a widely used technique to interrogate gene expression and cell fate, both in small and large animal models of research.
Laura Mezzanotte
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Semisynthetic, Multicolor Probes for Bioluminescence Imaging [PDF]
ACS OmegaBioluminescence imaging is a sensitive and noninvasive technique for monitoring molecular and cellular processes in living systems. NanoLuc is one of the brightest and most widely used luciferase enzymes; however, the native blue emission of NanoLuc limits its applications due to poor sample penetration.
Suong Tran +7 more
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NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing
Nature Communications, 2020 Conventional bioluminescence imaging usually operates in the visible region and its performance is limited by strong tissue absorption and scattering. Here, the authors present bioluminescence probes (BPs) with emission in the second near infrared (NIR ...
Lingfei Lu +8 more
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Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes [PDF]
Molecular Therapy: Nucleic Acids, 2013 Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Casey A Maguire +8 more
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Quantitative Assessment on Optical Properties as a Basis for Bioluminescence Imaging: An Experimental and Numerical Approach to the Transport of Optical Photons in Phantom Materials [PDF]
Sensors, 2023 Bioluminescence imaging (BLI) is a widely used technique in preclinical scientific research, particularly in the development of mRNA-based medications and non-invasive tumor diagnostics. It has become an essential tool in current science.
Tim Karstens +3 more
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Qualitative in vivo Bioluminescence Imaging
Bio-Protocol, 2018 Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in ...
Devbarna Sinha +2 more
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Use of a highly sensitive two-dimensional luminescence imaging system to monitor endogenous bioluminescence in plant leaves [PDF]
BMC Plant Biology, 2004 Background All living organisms emit spontaneous low-level bioluminescence, which can be increased in response to stress. Methods for imaging this ultra-weak luminescence have previously been limited by the sensitivity of the detection systems used ...
Flor-Henry Michel +3 more
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Enhancing Bioluminescence Imaging of Cultured Tissue Explants Using Optical Telecompression [PDF]
SensorsLong-term observation of single-cell oscillations within tissue networks is now possible by combining bioluminescence reporters with stable tissue explant culture techniques.
Jihwan Myung
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Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins. [PDF]
Chembiochem, 2021 AbstractBioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues.
Brennan CK +3 more
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Noninvasive Bioluminescence Imaging in Small Animals [PDF]
ILAR Journal, 2008 There has been a rapid growth of bioluminescence imaging applications in small animal models in recent years, propelled by the availability of instruments, analysis software, reagents, and creative approaches to apply the technology in molecular imaging. Advantages include the sensitivity of the technique as well as its efficiency, relatively low cost,
Kurt R Zinn +2 more
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