Results 21 to 30 of about 11,750 (282)

Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]

PLoS ONE, 2014
Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
doaj   +1 more source

Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays

Sensors, 2023
In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim, Tadaomi Furuta, Nobuo Kitada, Shojiro A. Maki   +3 more
doaj   +1 more source

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate

PLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye, Jun-ichi Sato, Yuiko Sahara-Miura, Yuri Tomabechi, Yuto Sumida, Shun-ichi Sekine, Mikako Shirouzu, Takamitsu Hosoya   +7 more
doaj   +2 more sources

Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]

PLoS ONE, 2011
BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte, Ivo Que, Eric Kaijzel, Bruce Branchini, Aldo Roda, Clemens Löwik   +5 more
doaj   +1 more source

Creation of Artificial Luciferases for Bioassays

, 2016
The present study demonstrates the creation of artificial luciferases (ALuc) for bioassays, inspired by a sequence alignment of copepod luciferases. Extraction of the consensus amino acids from the alignment enabled us to generate a series of ALucs with ...
Sung Bae Kim (572984), Masaki Torimura (278199), Hiroaki Tao (1868491)   +2 more
core   +2 more sources

A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models. [PDF]

PLoS Neglected Tropical Diseases, 2014
BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b.
Nick Van Reet, Hélène Van de Vyver, Patient Pati Pyana, Anne Marie Van der Linden, Philippe Büscher   +4 more
doaj   +1 more source

A higher spectral range of beetle bioluminescence with infraluciferin

Frontiers in Bioengineering and Biotechnology, 2022
Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul, Amit P. Jathoul, Bruce R. Branchini, James C. Anderson, James A. H. Murray   +4 more
doaj   +1 more source

Multicolor luciferase assay system: one-step monitoring of multiple gene expressions with a single substrate

BioTechniques, 2005
Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima, Takuma Kimura, Kazunori Sugata, Toshiteru Enomoto, Atsushi Asakawa, Hidehiro Kubota, Masaaki Ikeda, Yoshihiro Ohmiya   +7 more
doaj   +1 more source

Inhibition of luminescence activity of CTZ-utilizing luciferases with deaza-coelenterazine (daCTZ) analogs as inhibitors.

, 2022
Inhibition of luminescence activity of CTZ-utilizing luciferases with deaza-coelenterazine (daCTZ) analogs as inhibitors.
Mikako Shirouzu (272392), Yuto Sumida (1696852), Jun-ichi Sato (133357), Shun-ichi Sekine (1372875), Yuiko Sahara-Miura (1696846), Yuri Tomabechi (10644646), Satoshi Inouye (203922), Takamitsu Hosoya (205623)   +7 more
core   +1 more source

Codon optimized luciferases produce more light than non-optimized luciferases.

, 2014
BCG (104 cfu) expressing wild type click beetle red (CBRlux) or firefly (FFlux) luciferases or the same luciferases synthetically optimized for mycobacterial codon usage (opt) were examined in liquid culture for light production over time in the presence
Suat L. G. Cirillo (636106), MiHee Chang (636104), Katri P. Anttonen (636105), Kevin P. Francis (129761), Jeffrey D. Cirillo (376505)   +4 more
core   +1 more source