Results 21 to 30 of about 10,942 (259)
PLoS ONE, 2019 Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh +5 more
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Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]
PLoS ONE, 2011 BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte +5 more
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A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b.
Nick Van Reet +4 more
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A higher spectral range of beetle bioluminescence with infraluciferin
Frontiers in Bioengineering and Biotechnology, 2022 Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul +4 more
doaj +1 more source
BioTechniques, 2005 Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima +7 more
doaj +1 more source
Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]
PLoS ONE, 2018 Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li +12 more
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Clinical value of luciferase-based bioluminescence assay in diagnosis of Alport syndrome
Frontiers in PediatricsObjectivesAlport syndrome (AS) is an inherited kidney disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5. In this study, we aim to apply a split-luciferase bioluminescence assay to functionally assess COL4A3, COL4A4, or COL4A5 variants ...
Yue Cai +6 more
doaj +1 more source
Frontiers in Marine Science, 2021 The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse +3 more
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Beetle luciferases with naturally red- and blue-shifted emission
Life Science Alliance, 2018 New crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
César Carrasco-López +8 more
doaj +1 more source
Scientific Reports, 2021 Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes +5 more
doaj +1 more source