Results 21 to 30 of about 11,605 (288)
Near-infrared bioluminescence imaging of two cell populations in living mice
STAR Protocols, 2021 Summary: Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model.
Giorgia Zambito, Laura Mezzanotte
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Luciferase Controlled Protein Interactions
Journal of the American Chemical Society, 2021 Protein trafficking and protein-protein interactions (PPI) are central to regulatory processes in cells. Induced dimerization systems have been developed to control PPI and regulate protein trafficking (localization) or interactions. Chemically induced dimerization (CID) has proven to be a robust approach to control protein interactions and ...
Dalu Chang +4 more
openaire +3 more sources
Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]
PLoS ONE, 2014 Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
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Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase [PDF]
Molecular Imaging, 2011 Gaussia luciferase (Gluc) is a secreted reporter, and its expression in living animals can be assessed by in vivo bioluminescence imaging (BLI) or blood assays. We characterized Gluc as an in vivo reporter in comparison with firefly luciferase (Fluc). Mice were inoculated subcutaneously with tumor cells expressing both Fluc and Gluc and underwent Flue ...
Yusuke Inoue +7 more
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Luciferase‐free Luciferin Electrochemiluminescence
Angewandte Chemie International Edition, 2022 AbstractLuciferin is one of Nature's most widespread luminophores, and enzymes that catalyze luciferin luminescence are the basis of successful commercial “glow” assays for gene expression and metabolic ATP formation. Herein we report an electrochemical method to promote firefly's luciferin luminescence in the absence of its natural biocatalyst ...
Mattia Belotti +7 more
openaire +4 more sources
Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays
Sensors, 2023 In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim +3 more
doaj +1 more source
PLoS ONE, 2022 Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye +7 more
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Sensitive dual color in vivo bioluminescence imaging using a new red codon optimized firefly luciferase and a green click beetle luciferase. [PDF]
PLoS ONE, 2011 BACKGROUND: Despite a plethora of bioluminescent reporter genes being cloned and used for cell assays and molecular imaging purposes, the simultaneous monitoring of multiple events in small animals is still challenging. This is partly attributable to the
Laura Mezzanotte +5 more
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Creation of Artificial Luciferases for Bioassays
, 2016 The present study demonstrates the creation of artificial luciferases (ALuc) for bioassays, inspired by a sequence alignment of copepod luciferases. Extraction of the consensus amino acids from the alignment enabled us to generate a series of ALucs with ...
Sung Bae Kim (572984) +2 more
core +2 more sources
A higher spectral range of beetle bioluminescence with infraluciferin
Frontiers in Bioengineering and Biotechnology, 2022 Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved in vitro ...
Amit P. Jathoul +4 more
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