Results 31 to 40 of about 3,574 (243)
BioTechniques, 2005 Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima +7 more
doaj +1 more source
Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes [PDF]
, 2013 Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Badr, Christian E +8 more
core +2 more sources
Ricin trafficking in plant and mammalian cells [PDF]
, 2011 Ricin is a heterodimeric plant protein that is potently toxic to mammalian and many other eukaryotic cells. It is synthesized and stored in the endosperm cells of maturing Ricinus communis seeds (castor beans).
Lord, Mike, Spooner, Robert A.
core +2 more sources
Luminescence- and fluorescence-based complementation assays to screen for GPCR oligomerization : current state of the art [PDF]
, 2019 G protein-coupled receptors (GPCRs) have the propensity to form homo- and heterodimers. Dysfunction of these dimers has been associated with multiple diseases, e.g., pre-eclampsia, schizophrenia, and depression, among others.
Crans, René +4 more
core +1 more source
Gaussia Luciferase for Bioluminescence Tumor Monitoring in Comparison with Firefly Luciferase [PDF]
Molecular Imaging, 2011 Gaussia luciferase (Gluc) is a secreted reporter, and its expression in living animals can be assessed by in vivo bioluminescence imaging (BLI) or blood assays. We characterized Gluc as an in vivo reporter in comparison with firefly luciferase (Fluc). Mice were inoculated subcutaneously with tumor cells expressing both Fluc and Gluc and underwent Flue
Yusuke Inoue +7 more
openaire +3 more sources
Scientific Reports, 2021 Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes +5 more
doaj +1 more source
Frontiers in Marine Science, 2021 The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse +3 more
doaj +1 more source
Chemical Genetics Identify eIF2α Kinase Heme Regulated Inhibitor as Anti-Cancer Target [PDF]
, 2014 Translation initiation plays a critical role in cellular homeostasis, proliferation, differentiation and malignant transformation. Consistently, increasing the abundance of the eIF2·GTP·Met-tRNAi translation initiation complex transforms normal cells and
Aktas, Bertal H. +11 more
core +1 more source
Clinical value of luciferase-based bioluminescence assay in diagnosis of Alport syndrome
Frontiers in PediatricsObjectivesAlport syndrome (AS) is an inherited kidney disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5. In this study, we aim to apply a split-luciferase bioluminescence assay to functionally assess COL4A3, COL4A4, or COL4A5 variants ...
Yue Cai +6 more
doaj +1 more source
Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]
PLoS ONE, 2018 Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li +12 more
doaj +1 more source