Results 31 to 40 of about 11,605 (288)
BioTechniques, 2005 Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression.
Yoshihiro Nakajima +7 more
doaj +1 more source
, 2022 Inhibition of luminescence activity of CTZ-utilizing luciferases with deaza-coelenterazine (daCTZ) analogs as inhibitors.
Mikako Shirouzu (272392) +7 more
core +1 more source
Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]
PLoS ONE, 2018 Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li +12 more
doaj +1 more source
Engineering Luciferases for Assays and Imaging
, 2013 Luciferases have served a number of purposes in biomedical applications, including within reporter gene and split reporter complementation assays. These proteins, however, have not evolved for the purpose of biomedical research, and it is not surprising ...
Walls, Zachary F. +3 more
core +1 more source
Clinical value of luciferase-based bioluminescence assay in diagnosis of Alport syndrome
Frontiers in PediatricsObjectivesAlport syndrome (AS) is an inherited kidney disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5. In this study, we aim to apply a split-luciferase bioluminescence assay to functionally assess COL4A3, COL4A4, or COL4A5 variants ...
Yue Cai +6 more
doaj +1 more source
Frontiers in Marine Science, 2021 The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse +3 more
doaj +1 more source
Beetle luciferases with naturally red- and blue-shifted emission
Life Science Alliance, 2018 New crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
César Carrasco-López +8 more
doaj +1 more source
Scientific Reports, 2021 Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes +5 more
doaj +1 more source
iScience, 2021 Summary: For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of
Giorgia Zambito +10 more
doaj +1 more source
Codon optimized luciferases produce more light than non-optimized luciferases.
, 2014 BCG (104 cfu) expressing wild type click beetle red (CBRlux) or firefly (FFlux) luciferases or the same luciferases synthetically optimized for mycobacterial codon usage (opt) were examined in liquid culture for light production over time in the presence
Suat L. G. Cirillo (636106) +4 more
core +1 more source