Results 31 to 40 of about 11,750 (282)

Clinical value of luciferase-based bioluminescence assay in diagnosis of Alport syndrome

Frontiers in Pediatrics
ObjectivesAlport syndrome (AS) is an inherited kidney disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5. In this study, we aim to apply a split-luciferase bioluminescence assay to functionally assess COL4A3, COL4A4, or COL4A5 variants ...
Yue Cai, Ying-qi Lin, Xin-yu Kuang, Lei Sun, Meng-ying Li, Yu-jie Hu, Wen-yan Huang   +6 more
doaj   +1 more source

Optimized application of the secreted Nano-luciferase reporter system using an affinity purification strategy. [PDF]

PLoS ONE, 2018
Secreted Nano-luciferase (secNluc) is a newly engineered secreted luciferase that possesses advantages of high structural stability, long half-life, and glow-type kinetics together with high light emission intensity, and thus would become one of the most
JingZhe Li, ZhiLan Guo, Takashi Sato, Bo Yuan, YanYan Ma, Dan Qian, JuYing Zhong, MengMeng Jin, Peng Huang, LuYang Che, Yi Wang, Yan Lei, ChangZhen Liu   +12 more
doaj   +1 more source

Beetle luciferases with naturally red- and blue-shifted emission

Life Science Alliance, 2018
New crystal structures of red- and green blue–shifted beetle luciferases reveal that the color emission mechanism is dependent on the active site microenvironment affected by the conformation of loop regions.
César Carrasco-López, Juliana C Ferreira, Nathan M Lui, Stefan Schramm, Romain Berraud-Pache, Isabelle Navizet, Santosh Panjikar, Panče Naumov, Wael M Rabeh   +8 more
doaj   +1 more source

Photophore Distribution and Enzymatic Diversity Within the Photogenic Integument of the Cookie-Cutter Shark Isistius brasiliensis (Chondrichthyes: Dalatiidae)

Frontiers in Marine Science, 2021
The cookie-cutter shark Isistius brasiliensis (Squaliformes: Dalatiidae) is a deep-sea species that emits a blue luminescence ventrally, except at the level of a black band located beneath the jaw.
Jérôme Delroisse, Laurent Duchatelet, Patrick Flammang, Jérôme Mallefet   +3 more
doaj   +1 more source

Engineering Luciferases for Assays and Imaging

, 2013
Luciferases have served a number of purposes in biomedical applications, including within reporter gene and split reporter complementation assays. These proteins, however, have not evolved for the purpose of biomedical research, and it is not surprising ...
Walls, Zachary F., Loening, Andreas M., Andreas M. Loening, Zachary F. Walls   +3 more
core   +1 more source

Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity

Scientific Reports, 2021
Reporter gene assays are widely used to study cellular signaling and transcriptional activity. Few studies describe the use of reporter genes for studying cellular responses on complex body fluids, such as urine and blood.
M. Neefjes, B. A. C. Housmans, G. G. H. van den Akker, L. W. van Rhijn, T. J. M. Welting, P. M. van der Kraan   +5 more
doaj   +1 more source

Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging

iScience, 2021
Summary: For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of
Giorgia Zambito, Mary P. Hall, Monika G. Wood, Natasa Gaspar, Yanto Ridwan, Fabio F. Stellari, Ce Shi, Thomas A. Kirkland, Lance P. Encell, Clemens Löwik, Laura Mezzanotte   +10 more
doaj   +1 more source

Luciferase Controlled Protein Interactions

Journal of the American Chemical Society, 2021
Protein trafficking and protein-protein interactions (PPI) are central to regulatory processes in cells. Induced dimerization systems have been developed to control PPI and regulate protein trafficking (localization) or interactions. Chemically induced dimerization (CID) has proven to be a robust approach to control protein interactions and ...
Dalu Chang, Suihan Feng, Vladimir Girik, Howard Riezman, Nicolas Winssinger   +4 more
openaire   +3 more sources

Translophagy—A potential link between autophagy impairment and translational errors

FEBS Letters, EarlyView.
Neurodegenerative diseases are characterised by the accumulation of abnormal proteins and protein aggregates, but their origin often remains unknown. We propose that selective autophagy removes damaged protein‐making machinery, preventing errors during protein synthesis.
Mykola V. Korolchuk, Gailing Ma, Robin Antrobus, Diego Manni, Marco Trevisan‐Herraz, Mustafa Khwaja, Max Brown, Gavin D. Richardson, Masaaki Komatsu, Daniel Erskine, Viktor I. Korolchuk, Tetsushi Kataura   +11 more
wiley   +1 more source

Group entropy analysis of luciferases.

, 2018
Group entropy analysis of luciferases.
Danielle Leatherby (5714819), Louis Clark (5714816), John Perozich (5714825), Alexander J. Ropelewski (237444), Elizabeth Krilich (5714822)   +4 more
core   +1 more source