Results 11 to 20 of about 11,605 (288)
Approaches to engineer stability of beetle luciferases
Computational and Structural Biotechnology Journal, 2012 Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas.
Mikhail Koksharov, Natalia Ugarova
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Leaving the Dark Side? Insights Into the Evolution of Luciferases [PDF]
Frontiers in Marine Science, 2021 Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, a luciferin substrate, an oxygen derivative, and a specialised luciferase enzyme.
Jérôme Delroisse +3 more
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PLoS ONE, 2019 Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh +5 more
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Life, 2023 Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova +3 more
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Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence
Molecular Imaging, 2004 Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression.
Brenda Baggett +6 more
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A panel of Trypanosoma brucei strains tagged with blue and red-shifted luciferases for bioluminescent imaging in murine infection models. [PDF]
PLoS Neglected Tropical Diseases, 2014 BACKGROUND:Genetic engineering with luciferase reporter genes allows monitoring Trypanosoma brucei (T.b.) infections in mice by in vivo bioluminescence imaging (BLI). Until recently, luminescent T.b.
Nick Van Reet +4 more
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Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases [PDF]
BMC Biotechnology, 2008 Background Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red ...
Ohmiya Yoshihiro +3 more
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A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence [PDF]
Open Biology, 2017 Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes.
Jérôme Delroisse +6 more
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Expanding luciferase reporter systems for cell-free protein expression
Scientific Reports, 2022 Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-translation (TXTL) systems, for research and practical applications such as engineering genetic parts, validating genetic circuits, and biosensor outputs. Currently,
Wakana Sato +4 more
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Multiplex quadruple bioluminescent assay system
Scientific Reports, 2022 Bioluminescence (BL) is unique cold body radiation of light, generated by luciferin–luciferase reactions and commonly used in various bioassays and molecular imaging.
Genta Kamiya +3 more
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