Results 11 to 20 of about 3,574 (243)

Multiplex quadruple bioluminescent assay system

open access: yesScientific Reports, 2022
Bioluminescence (BL) is unique cold body radiation of light, generated by luciferin–luciferase reactions and commonly used in various bioassays and molecular imaging.
Genta Kamiya   +3 more
doaj   +1 more source

Near-infrared bioluminescence imaging of two cell populations in living mice

open access: yesSTAR Protocols, 2021
Summary: Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model.
Giorgia Zambito, Laura Mezzanotte
doaj   +1 more source

Propensity scores for prediction and characterization of bioluminescent proteins from sequences. [PDF]

open access: yesPLoS ONE, 2014
Bioluminescent proteins (BLPs) are a class of proteins with various mechanisms of light emission such as bioluminescence and fluorescence from luminous organisms.
Hui-Ling Huang
doaj   +1 more source

Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens. [PDF]

open access: yes, 2009
A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations.
Stekel, Dov J, Welham, Patricia A
core   +1 more source

Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays

open access: yesSensors, 2023
In this study, a series of new artificial luciferases (ALucs) was created using sequential insights on missing peptide blocks, which were revealed using the alignment of existing ALuc sequences.
Sung-Bae Kim   +3 more
doaj   +1 more source

Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy. [PDF]

open access: yes, 2014
Time-lapse fluorescence microscopy is an important tool for measuring in vivo gene dynamics in single cells. However, fluorescent proteins are limited by slow chromophore maturation times and the cellular autofluorescence or phototoxicity that arises ...
Aydin, M   +3 more
core   +1 more source

Reversible low-light induced photoswitching of crowned spiropyran-DO3A complexed with gadolinium(III) ions. [PDF]

open access: yes, 2012
Photoswitchable spiropyran has been conjugated to the crowned ring system DO3A, which improves its solubility in dipolar and polar media and stabilizes the merocyanine isomer. Adding the lanthanide ion gadolinium(III) to the macrocyclic ring system leads
Brueggemann, Chantal   +6 more
core   +4 more sources

Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate

open access: yesPLoS ONE, 2022
Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs.
Satoshi Inouye   +7 more
doaj   +2 more sources

Recent Progress in Optical Sensors for Biomedical Diagnostics [PDF]

open access: yes, 2020
In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject.
Altintas, Zeynep, Pirzada, Muqsit
core   +1 more source

Kinetics of the thermal inactivation and the refolding of bacterial luciferases in Bacillus subtilis and in Escherichia coli differ.

open access: yesPLoS ONE, 2019
Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh   +5 more
doaj   +1 more source

Home - About - Disclaimer - Privacy