Results 11 to 20 of about 11,750 (282)

Approaches to engineer stability of beetle luciferases [PDF]

open access: yesComputational and Structural Biotechnology Journal, 2012
Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas.
Mikhail Koksharov, Natalia Ugarova
doaj   +4 more sources

Leaving the Dark Side? Insights Into the Evolution of Luciferases [PDF]

open access: yesFrontiers in Marine Science, 2021
Bioluminescence—i.e., the emission of visible light by living organisms—is defined as a biochemical reaction involving, at least, a luciferin substrate, an oxygen derivative, and a specialised luciferase enzyme.
Jérôme Delroisse   +3 more
doaj   +4 more sources

Kinetics of the thermal inactivation and the refolding of bacterial luciferases in Bacillus subtilis and in Escherichia coli differ.

open access: yesPLoS ONE, 2019
Here we present a study of the thermal inactivation and the refolding of the proteins in Gram positive Bacillus subtilis. To enable use of bacterial luciferases as the models for protein thermal inactivation and refolding in B.
Eugeny Gnuchikh   +5 more
doaj   +2 more sources

Localization of the Catalytic Domain of Copepod Luciferases: Analysis of Truncated Mutants of the Metridia longa Luciferase

open access: yesLife, 2023
Luciferases from copepods Metridia longa and Gaussia princeps are successfully used as bioluminescent reporters for in vivo and in vitro assays. Here, we report the minimal sequence of copepod luciferases required for bioluminescence activity that was ...
Svetlana V. Markova   +3 more
doaj   +2 more sources

Thermostability of Firefly Luciferases Affects Efficiency of Detection by in Vivo Bioluminescence

open access: yesMolecular Imaging, 2004
Luciferase from the North American firefly ( Photinis pyralis ) is a useful reporter gene in vivo, allowing noninvasive imaging of tumor growth, metastasis, gene transfer, drug treatment, and gene expression.
Brenda Baggett   +6 more
doaj   +2 more sources

Luciferases : Cooperativa de divulgació científica feminista [PDF]

open access: yes, 2020
Màster Oficial en Gestió Cultural, Universitat de Barcelona. Facultat d’Economia i Empresa, curs: 2019-2020, Tutor: Anna Villarroya PlanasLuciferases és una cooperativa sense ànim de lucre que aposta per relacionar ciència i cultura aportant llum crítica
Ferrusola Bial, Judit
core   +7 more sources

A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence [PDF]

open access: yesOpen Biology, 2017
Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes.
Jérôme Delroisse   +6 more
doaj   +1 more source

Expanding luciferase reporter systems for cell-free protein expression

open access: yesScientific Reports, 2022
Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-translation (TXTL) systems, for research and practical applications such as engineering genetic parts, validating genetic circuits, and biosensor outputs. Currently,
Wakana Sato   +4 more
doaj   +1 more source

Multiplex quadruple bioluminescent assay system

open access: yesScientific Reports, 2022
Bioluminescence (BL) is unique cold body radiation of light, generated by luciferin–luciferase reactions and commonly used in various bioassays and molecular imaging.
Genta Kamiya   +3 more
doaj   +1 more source

Near-infrared bioluminescence imaging of two cell populations in living mice

open access: yesSTAR Protocols, 2021
Summary: Multicolor bioluminescence imaging using near-infrared emitting luciferases is an attractive application to detect two cell populations within one animal model.
Giorgia Zambito, Laura Mezzanotte
doaj   +1 more source

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