Results 61 to 70 of about 3,574 (243)
Molecular Oncology, EarlyView.Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau +8 more
wiley +1 more source
BMC Biotechnology, 2008 Background Luciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red ...
Ohmiya Yoshihiro +3 more
doaj +1 more source
Dual Luciferase Reporter Gene [PDF]
, 2011 Luciferase is an enzyme that is used in bioluminescence. Luciferase most famous form is the one found in fireflies. In this form Luciferase works in a very unique way.
Bach, Christian, Bolanos, Edwin
core
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
FEBS Open Bio, EarlyView.Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source
Optimisation of bioluminescent reporters for use with mycobacteria.
PLoS ONE, 2010 BackgroundMycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology ...
Nuria Andreu +10 more
doaj +1 more source
Molecular Therapy: Oncolytics, 2021 Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz +10 more
doaj +1 more source
Cloning, Sequencing, and Characterization of Luciola italica Luciferase [PDF]
, 2006 The characteristic yellow-green light of a firefly is the result of a multi-step reaction catalyzed by the luciferase enzyme. This enzyme has many applications in the biomedical field and ongoing work is being done to alter its properties to better fit ...
DeAngelis, Jennifer P.
core +1 more source
The 5 ' untranslated region of protein kinase C delta directs translation by an internal ribosome entry segment that is most active in densely growing cells and during apoptosis. [PDF]
, 2002 Protein kinase Cdelta (PKCdelta) is a member of the PKC family of phospholipid-dependent serine/threonine kinases and is involved in cell proliferation, apoptosis, and differentiation.
Morrish, B.C., Rumsby, M.G.
core +2 more sources
FEBS Open Bio, EarlyView.A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti +3 more
wiley +1 more source
Examining multiple cellular pathways at once using multiplex hextuple luciferase assaying
Nature Communications, 2019 Multiplexed detection of luciferase-based sensors in the same sample is challenging and limited by the substrates’ emission spectra. Here the authors establish a system based on three different luciferases and sequential detection to achieve measurements
Alejandro Sarrion-Perdigones +5 more
doaj +1 more source