Results 71 to 80 of about 10,942 (259)

Genetically Modified Coelenterazine-Dependent Luciferases as Reporters for In Vitro Assay [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
Among the most important tasks of modern biotechnology is the development of new analytical techniques suitable for highly sensitive and specific detection of diagnostically important targets rapidly expanding their range with the progress in biomedical ...
Ludmila A. Frank, Eugenia E. Bashmakova, Vasilisa V. Krasitskaya, Alexander N. Kudryavtsev   +3 more
doaj  

CPP–PEG‐Guided Surface Engineering of Mitochondria Enables Efficient Cellular Uptake and Respiratory Modulation

Advanced Materials Interfaces, EarlyView.
PEG‐guided mitochondrial surface engineering supports structural stabilization and controlled presentation of cell‐penetrating peptides. CPP–PEG‐modified mitochondria exhibit enhanced cellular uptake and uptake‐associated respiratory modulation, providing a proof‐of‐concept framework for modular organelle engineering and future bioenergetic modulation ...
Masahiro Shiraishi, Yukari Muramatsu, Akihiro Nakaya, Yuji Maruo, Rick C. Tsai, Masashi Suganuma, Hisashi Ohta, Atsuhito Takeda, Hideyoshi Harashima, Yuma Yamada   +9 more
wiley   +1 more source

Brazilian Bioluminescent Beetles: Reflections on Catching Glimpses of Light in the Atlantic Forest and Cerrado

Anais da Academia Brasileira de Ciências
Bioluminescence - visible and cold light emission by living organisms - is a worldwide phenomenon, reported in terrestrial and marine environments since ancient times.
ETELVINO J.H. BECHARA, CASSIUS V. STEVANI   +1 more
doaj   +1 more source

High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases.

PLoS ONE, 2009
Networks of protein-protein interactions play key roles in numerous important biological processes in living subjects. An effective methodology to assess protein-protein interactions in living cells of interest is protein-fragment complement assay (PCA).
Naoki Hida, Muhammad Awais, Masaki Takeuchi, Naoto Ueno, Mayuri Tashiro, Chiyo Takagi, Tanuja Singh, Makoto Hayashi, Yoshihiro Ohmiya, Takeaki Ozawa   +9 more
doaj   +1 more source

Stromal‐Derived IL‐8 Promotes M2 Macrophage Polarization via the RhoA/MRTF‐A/SRF Transcriptional Axis to Impair CD8+ T Cell Cytotoxicity in Lung Cancer

Advanced Science, EarlyView.
In the recondite pulmonary neoplastic milieu, DESMIN+ cancer‐associated fibroblasts function as the paramount stromal source of IL‐8, precipitating a deleterious immunological metamorphosis by engaging CXCR2 receptors on the myeloid lineage. This paracrine stimulus triggers a mechanotransduction cascade characterized by RhoA‐dependent actin ...
Xuyu Gu, Qiyu Fang, Jia Yu, Ting Yu, Huashan Shi, Kaiqi Jin, Lei Jiang, Wentian Zhang   +7 more
wiley   +1 more source

Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

Scientific Reports, 2017
Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate ...
Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim   +5 more
doaj   +1 more source

Gut Microbiota Regulates Systemic Inflammatory Response and Compensatory Anti‐Inflammatory Response Syndromes by Targeting PF4+ Macrophages in Acute Pancreatitis

Advanced Science, Volume 13, Issue 46, 17 August 2026.
ABSTRACT Acute pancreatitis (AP) begins with pancreatic local inflammation, leading to the onset of systemic inflammatory response syndrome (SIRS), followed by compensatory anti‐inflammatory response syndrome (CARS), which causes immune paralysis and higher mortality rate.
Liwei Liu, Guanqun Li, Dongxu Lu, Haoran Ding, Tianqi Lu, Yuhang Sui, Can Zhang, Yu Xie, Rui Kong, Hua Chen, Xuewei Bai, Hongtao Tan, Dongbo Xue, Xianzhi Meng, Le Li, Bei Sun   +15 more
wiley   +1 more source

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

Biology
The L. fermentum U-21 strain, known for secreting chaperones into the extracellular milieu, emerges as a promising candidate for the development of novel therapeutics termed disaggregases for Parkinson’s disease.
Rahaf N. Al Ebrahim, Maria G. Alekseeva, Sergey V. Bazhenov, Vadim V. Fomin, Dilara A. Mavletova, Andrey A. Nesterov, Elena U. Poluektova, Valeriy N. Danilenko, Ilya V. Manukhov   +8 more
doaj   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

A View on the Active Site of Firefly Luciferase

Natural Product Communications, 2008
The bioluminescent enzyme firefly luciferase has been the subject of detailed biochemical studies since the late 1940s. These studies have culminated in the cloning and sequence determination of some 161 different c-DNAs and/or genes for firefly ...
Franklin R. Leach
doaj   +1 more source