Results 71 to 80 of about 11,605 (288)
Building Biological Flashlights: Orthogonal Luciferases and Luciferins for in Vivo Imaging [PDF]
, 2019 Bioluminescence is widely used for real-time imaging in living organisms. This technology features a light-emitting reaction between enzymes (luciferases) and small molecule substrates (luciferins).
Williams, Sierra J, Prescher, Jennifer A
core +1 more source
mLife, 2023 Focal and systemic infections are serious threats to human health. Preclinical models enable the development of new drugs and therapeutic regimens. In vivo, animal bioluminescence (BL) imaging has been used with bacterial reporter strains to evaluate ...
Weilong Shang +17 more
doaj +1 more source
Advanced Science, EarlyView.This study reveals that metformin promotes glucuronic acid metabolism in lung adenocarcinoma by activating UGDH S476 phosphorylation and enhancing the conversion of UDPG to UDPGA based on metabolomics analysis. Through compound virtual screening, it is found that plantainoside targeting UGDH downstream UXS1 leads to UDPGA toxicity accumulation ...
Qihai Sui +14 more
wiley +1 more source
Advanced Science, EarlyView.ABSTRACT Acute pancreatitis (AP) begins with pancreatic local inflammation, leading to the onset of systemic inflammatory response syndrome (SIRS), followed by compensatory anti‐inflammatory response syndrome (CARS), which causes immune paralysis and higher mortality rate.
Liwei Liu +15 more
wiley +1 more source
, 2021 Detecting the folding/unfolding pathways of biological macromolecules is one of the urgent problems of molecular biophysics. The unfolding of bacterial luciferase from Vibrio harveyi is well-studied, unlike that of Photobacterium leiognathi, despite the ...
Dmitry V. Gulnov +7 more
core +1 more source
Advanced Science, EarlyView.This study identifies the HDAC6/GATA4/HtrA1 axis as a critical driver of cellular senescence in the inner ear. GATA4 nuclear translocation, facilitated by HDAC6 downregulation, transcriptionally activates HtrA1, promoting hair cell senescence, SASP, and audio‐vestibular dysfunction in models of Ménière's disease and age‐related audio‐vestibular ...
Na Zhang +16 more
wiley +1 more source
Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging [PDF]
, 2018 New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells.
Zhang, Brendan S +7 more
core +1 more source
, 2022 Reaction products of coelenteramine (CTM) and coelenteramide (CTMD) from coelenterazine (CTZ) by incubation of various CTZ-utilizing luciferases by HPLC analysis.
Mikako Shirouzu (272392) +7 more
core +1 more source
Advanced Science, EarlyView.We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel +16 more
wiley +1 more source
Purification and Properties of Bacterial Luciferases
Journal of Biological Chemistry, 1972 Abstract A newly developed procedure is described for the isolation and purification on a large scale of luciferases from two different bioluminescent bacteria, Pf and MAV. In only three major steps luciferase can be obtained in greater than 95% purity. An improved method for the isolation of subunits in milligram quantities by column chromatography in
A, Gunsalus-Miguel +4 more
openaire +2 more sources