Results 81 to 90 of about 11,750 (282)

A dual-luciferase bioluminescence system for the assessment of cellular therapies

Molecular Therapy: Oncology
Bioluminescence imaging is a well-established platform for evaluating engineered cell therapies in preclinical studies. However, despite the discovery of new luciferases and substrates, optimal combinations to simultaneously monitor two cell populations ...
Alejandro G. Torres Chavez, Mary K. McKenna, Kishore Balasubramanian, Lisa Riffle, Nimit L. Patel, Joseph D. Kalen, Brad St. Croix, Ann M. Leen, Pradip Bajgain   +8 more
doaj   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source

Pyrearinus termitilluminans larval click beetle luciferase: Active site properties, structure and function relationships and comparison with other beetle luciferases

, 2009
Several beetle luciferases have been cloned and sequenced. However, most studies on structure and function relationships and bioanalytical applications were done with firefly luciferases, which are pH sensitive.
Silva Neto, A. J., Arnoldi, F. G C, Scorsato, V., Viviani, V. R., Silva Neto, A. J. [UNESP]   +4 more
core   +1 more source

Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging [PDF]

, 2018
New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells.
Zhang, Brendan S, Jennifer A. Prescher, Brendan S. Zhang, McCutcheon, David C, Prescher, Jennifer A, Jones, Krysten A, Krysten A. Jones, David C. McCutcheon   +7 more
core   +1 more source

Chemical Screening Identifies Danofloxacin as a Self‐Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1‐Mediated Deacetylation of Tert and Prdm10

Advanced Science, EarlyView.
This study identified Danofloxacin as a novel supporter of the maintenance of mouse embryonic stem cell (ESC) pluripotency. It functions partially by inhibiting HDAC1, which increases specific histone acetylation marks and activates key genes like Tert and Prdm10.
Yan Zhang, Junxiang Ji, Baoxing Dong, Zhenhua Zhu, Xucheng Lv, Peng Chen, Wenqiang Liu, Min Zhou, Shou‐Dong Ye   +8 more
wiley   +1 more source

Coixenolide Enhances Antitumor Immunity of Cytotoxic CD8+ T Cells by Inhibiting S100A8/A9‐CD36 Axis in Obesity‐Associated Pancreatic Adenocarcinoma

Advanced Science, EarlyView.
Pancreatic adenocarcinoma (PAAD) remains highly lethal with limited treatment options. This study demonstrates that coixenolide, a bioactive compound from Coix lacryma‐jobi L. and a key component of the clinically approved Kanglaite injection, exhibits enhanced antitumor efficacy in high‐fat diet (HFD)‐induced obese mice bearing PAAD tumors compared to
Kaidi Chen, Rong Chen, Wei Li, Bin Song, Ruiying Zhang, Lewei Dai, Yuwei Qi, Ruqian Chen, Yifan Xu, Hangbin Li, Jingyi Huang, Fangping Wu, Jianan Peng, Xinyu Wang, Yang Xiong, Chaofeng Mu, Guilin Cheng, Zhaoshen Li, Shanming Ruan, Lei Huang, Mancang Gu   +20 more
wiley   +1 more source

Aspectos comparativos das luciferases pH-insensitivas de Pyrearinus termitilluminans (Coleoptera: Elateridae) e Phrixotrix spp (Coleoptera: Phengodidae): expressão, purificação e propriedades do sítio ativo

, 2009
Neste trabalho foi feita a expressão e purificação das luciferases recombinantes do elaterídeo Pyrearinus termitilluminans e dos fengodídeos Phrixotrix hirtus e Phrixotrix viviani, e a determinação e comparação de suas propriedades físico-químicas ...
Silva Neto, Antonio Joaquim da [UNESP]
core   +3 more sources

Genetically Modified Coelenterazine-Dependent Luciferases as Reporters for In Vitro Assay [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
Among the most important tasks of modern biotechnology is the development of new analytical techniques suitable for highly sensitive and specific detection of diagnostically important targets rapidly expanding their range with the progress in biomedical ...
Ludmila A. Frank, Eugenia E. Bashmakova, Vasilisa V. Krasitskaya, Alexander N. Kudryavtsev   +3 more
doaj  

Compensatory Mitophagy via NEDD4/HIF‐1α/BNIP3 Pathway Restrains EndMT and Renal Allograft Interstitial Fibrosis Induced by TNFα

Advanced Science, EarlyView.
The role of TNFα in the process of renal allograft interstitial fibrosis is complex and multifaceted. As it can promote renal allograft interstitial fibrosis by inducing mitochondrial dysfunction and EndMT, and also mediating compensatory mitophagy through the NEDD4–HIF‐1α–BNIP3 pathway, which clears damaged mitochondria and inhibits EndMT, thereby ...
Dengyuan Feng, Qinghuan Shen, Junqi Zhang, Jiawen Liu, Zeping Gui, Zijie Wang, Qianguang Han, Shuang Fei, Hao Chen, Li Sun, Jun Tao, Zhijian Han, Xiaobing Ju, Min Gu, Jianjian Zhang, Ruoyun Tan   +15 more
wiley   +1 more source

Brazilian Bioluminescent Beetles: Reflections on Catching Glimpses of Light in the Atlantic Forest and Cerrado

Anais da Academia Brasileira de Ciências
Bioluminescence - visible and cold light emission by living organisms - is a worldwide phenomenon, reported in terrestrial and marine environments since ancient times.
ETELVINO J.H. BECHARA, CASSIUS V. STEVANI   +1 more
doaj   +1 more source