Results 91 to 100 of about 11,750 (282)

High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases.

PLoS ONE, 2009
Networks of protein-protein interactions play key roles in numerous important biological processes in living subjects. An effective methodology to assess protein-protein interactions in living cells of interest is protein-fragment complement assay (PCA).
Naoki Hida, Muhammad Awais, Masaki Takeuchi, Naoto Ueno, Mayuri Tashiro, Chiyo Takagi, Tanuja Singh, Makoto Hayashi, Yoshihiro Ohmiya, Takeaki Ozawa   +9 more
doaj   +1 more source

Modulation of miR‐23b Wnt/β‐catenin Axis Strengthens Endothelial Barrier Properties

Advanced Science, EarlyView.
Early blood‐brain barrier (BBB) disruption contributes to stroke and CNS disease pathology. miR‐23b was identified as a regulator of BBB integrity in brain endothelial cells. Inhibition of miR‐23b enhanced barrier‐associated properties, promoted repair‐related signaling, and reduced BBB leakage in experimental stroke models, supporting further ...
Victor Anthony Martinez, Rose E. Presby, Sara Nakanishi, Pallavi Gaur, Ugur Akcan, Johannes Daugaard Krog, Iben Daugaard Krog, Lipi Das, David S. Johnson, Danny Jamoul, Douglas Jury, Brian Lawson, Jacob Körbelin, Lewis M. Brown, Vilas Menon, Dritan Agalliu, Irene Munk Pedersen   +16 more
wiley   +1 more source

Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

Scientific Reports, 2017
Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate ...
Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim   +5 more
doaj   +1 more source

Integrating Genetics With Epidemiological Measurements Identifies Burden QTLs of Inflammatory Bowel Disease across 20 Countries

Advanced Science, EarlyView.
The burden quantitative trait locus (bQTL) analysis integrates epidemiological data from GBD study with genotype of 2 621 978 SNPs from 2414 individuals in 1000 Genomes Project across 20 countries. rs7633471 shows the strongest effect that each increase in centi‐allele frequency of the allele C associated with 10.00 decrease of age‐standardized ...
Chen Sun, Siyu Wei, Junxian Tao, Haiyan Chen, Chi Yan, Jiacheng Wang, Jing Xu, Lian Duan, Yuanbo Zhan, Yuping Zou, Ruilin Li, Linna Yuan, Wei She, Songlin Lu, Ning Wang, Qinduo Ren, Yan Guo, Yuan Xu, Chang Wang, Hongsheng Tian, Chen Zhang, Yu Dong, Guoping Tang, Zhenwei Shang, Yongshuai Jiang, Wenhua Lv, Mingming Zhang, Hongchao Lyu   +27 more
wiley   +1 more source

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

Biology
The L. fermentum U-21 strain, known for secreting chaperones into the extracellular milieu, emerges as a promising candidate for the development of novel therapeutics termed disaggregases for Parkinson’s disease.
Rahaf N. Al Ebrahim, Maria G. Alekseeva, Sergey V. Bazhenov, Vadim V. Fomin, Dilara A. Mavletova, Andrey A. Nesterov, Elena U. Poluektova, Valeriy N. Danilenko, Ilya V. Manukhov   +8 more
doaj   +1 more source

Copper‐Driven Epithelial Barrier Disruption: A Novel Mechanism of COPD Acute Exacerbations Mediated by the TNF‐α/ATP7A Axis

Advanced Science, EarlyView.
This study reveals a copper‐driven mechanism of COPD acute exacerbations mediated by the TNF‐α/ATP7A axis. TNF‐α suppresses the copper transporter ATP7A via competitive NF‐κB/CREB1/CBP signaling, resulting in intracellular copper accumulation, mitochondrial oxidative stress, and epithelial barrier disruption.
Xinru Xiao, Yongzhe Hao, Ziqi Ding, Zhipeng Wang, Yujia Shi, Yanhua Huang, Chunjian Qi, Qian Zhang   +7 more
wiley   +1 more source

Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays

Frontiers in Chemistry
Bioluminescent high-throughput screening (HTS) assays, based largely on the activity of firefly (FLuc), Renilla (RLuc), and/or NanoLuc (NLuc) luciferases, are widely utilised in research and drug discovery. In this study, we quantify the luciferase-based
Francesca Canyelles i Font, Krzysztof Żukowski, Masroor A. Khan, Dorota Kwiatek, Jacek L. Kolanowski   +4 more
doaj   +1 more source

A View on the Active Site of Firefly Luciferase

Natural Product Communications, 2008
The bioluminescent enzyme firefly luciferase has been the subject of detailed biochemical studies since the late 1940s. These studies have culminated in the cloning and sequence determination of some 161 different c-DNAs and/or genes for firefly ...
Franklin R. Leach
doaj   +1 more source

Synthetic Toll‐Like Receptors for Control of Innate Immunity With Far‐Red Light

Advanced Science, EarlyView.
Synthetic Toll‐like receptors (TLRs) are engineered by replacing their natural sensing regions with a far‐red‐light‐responsive module from a bacterial phytochrome. These synthetic receptors enable light‐controlled regulation of immune signaling in mammalian cells.
Anna V. Leopold, Vladislav V. Verkhusha
wiley   +1 more source

TRAIP Mediates Alcohol‐Induced Liver Injury through Regulating β‐catenin Ubiquitin Degradation via Direct Interaction

Advanced Science, EarlyView.
This study reveals that NF‐κB1‐driven TRAIP upregulation in ALD correlates with disease severity. Mechanistically, TRAIP directly binds β‐catenin via its CC domain and promotes its K48‐linked ubiquitination and degradation, which is independent of the GSK3β/β‐TrCP pathway.
Zhan Wu, Mingjiang Liu, Lijuan Liao, Zhaohong Mo, Wenli Xu, Linfeng Mao, Yubing Chen, Qiuli Xie, Ming Yao, Shilian Chen, Stephen Tomlinson, Sebastian Mueller, Songqing He, Guandou Yuan   +13 more
wiley   +1 more source