Results 91 to 100 of about 11,605 (288)

Targeting the PDK1/c‐Myc/SOX10 Signaling in Oligodendrocytes Alleviates Neuropathic Pain

Advanced Science, EarlyView.
This work reveals that oligodendrocyte homeostasis, mediated by PDK1, is a critical determinant of neuropathic pain (NPP) pathogenesis. Disruption of PDK1 in oligodendrocytes impairs SOX10‐dependent myelination programs through c‐Myc accumulation, leading to disrupted myelination and the pathophysiology of NPP.
Pingping Qiao, Lifang Guo, Guochao Yang, Chaoli Huang, He Wang, Jian‐Jun Yang, Guiquan Chen, Yimin Hu   +7 more
wiley   +1 more source

Catalytic mechanism for Renilla-type luciferases

, 2022
The widely used coelenterazine-powered Renilla luciferase was discovered over 40 years ago but the oxidative mechanism by which it generates blue photons remains unclear.
Neugebauer, Petr, Stuchla, Marketa, Schenkmayerova, Andrea, Martin Marek, Zbynek Prokop, Santana, Vinicius, Pimchai Chaiyen, Bednar, David, Toul, Martin, Gaspar P. Pinto, Damborsky, Jiri, Chaiyen, Pimchai, Janin, Yves, Pinto, Gaspar, Glwadys Gagnot, Vinicius T. Santana, Andrea Schenkmayerova, Marek, Martin, Pluskal, Daniel, Jiri Damborsky, Prokop, Zbynek, Petr Neugebauer, Daniel Pluskal, Racha Baatallah, David Bednar, Baatallah, Racha, Martin Toul, Yves L. Janin, Marketa Stuchla, Gagnot, Glwadys   +29 more
core   +1 more source

PRMT1‐Mediated LDHA Methylation Drives STAT3 Lactylation to Orchestrate Intestinal Inflammation and Tumorigenesis

Advanced Science, EarlyView.
This study identifies an immunometabolic axis wherein SAM‐driven PRMT1 methylates LDHA, enhancing its activity. The resultant lactate induces STAT3 K709 lactylation, which stabilizes an active conformation to promote STAT3 phosphorylation and IL‐10 expression.
Hui Wang, Mengyu Zhang, Weipeng Gong, Jiaxuan Wu, Junping Zhang, Wenxiu Zhang, Yue Liu, Kui Wang, Canhua Huang, Jun Zhou, Sijin Wu, Yan Li, Tianliang Li   +12 more
wiley   +1 more source

Enhanced Glycolysis‐Driven Histone H3K18 Lactylation Regulates Epileptogenesis by Modulating the E3 Ubiquitin Ligase COP1

Advanced Science, EarlyView.
Neuronal PKM2‐driven glycolysis generates excess lactate that triggers histone H3K18 lactylation (H3K18la), establishing a pathogenic metabolic‐epigenetic axis in epilepsy. Elevated H3K18la enriches the Cop1 promoter, transcriptionally upregulating the E3 ubiquitin ligase COP1, which subsequently drives proteasomal degradation of GABAARβ2 and impairs ...
Yuan Meng, Yanlin Guo, Liumi Jiang, Jing Luo, Yan Liu, Ziwei Yuan, Pingyang Ke, Ran Duan, Fei Xiao   +8 more
wiley   +1 more source

Genetically Modified Coelenterazine-Dependent Luciferases as Reporters for In Vitro Assay [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
Among the most important tasks of modern biotechnology is the development of new analytical techniques suitable for highly sensitive and specific detection of diagnostically important targets rapidly expanding their range with the progress in biomedical ...
Ludmila A. Frank, Eugenia E. Bashmakova, Vasilisa V. Krasitskaya, Alexander N. Kudryavtsev   +3 more
doaj  

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source

A new firefly luciferase with bimodal spectrum: Identification of structural determinants of spectral pH-sensitivity in firefly luciferases

, 2005
Fireflies emit flashes in the green-yellow region of the spectrum for the purpose of sexual attraction. The bioluminescence color is determined by the luciferases.
Viviani, Vadim R. [UNESP], Oehlmeyer, T. L., Brochetto-Braga, M. R., Arnoldi, F. G C [UNESP], Arnoldi, F. G C, Oehlmeyer, T. L. [UNESP], Brochetto-Braga, M. R. [UNESP], Viviani, Vadim R.   +7 more
core   +1 more source

Brazilian Bioluminescent Beetles: Reflections on Catching Glimpses of Light in the Atlantic Forest and Cerrado

Anais da Academia Brasileira de Ciências
Bioluminescence - visible and cold light emission by living organisms - is a worldwide phenomenon, reported in terrestrial and marine environments since ancient times.
ETELVINO J.H. BECHARA, CASSIUS V. STEVANI   +1 more
doaj   +1 more source

High-sensitivity real-time imaging of dual protein-protein interactions in living subjects using multicolor luciferases.

PLoS ONE, 2009
Networks of protein-protein interactions play key roles in numerous important biological processes in living subjects. An effective methodology to assess protein-protein interactions in living cells of interest is protein-fragment complement assay (PCA).
Naoki Hida, Muhammad Awais, Masaki Takeuchi, Naoto Ueno, Mayuri Tashiro, Chiyo Takagi, Tanuja Singh, Makoto Hayashi, Yoshihiro Ohmiya, Takeaki Ozawa   +9 more
doaj   +1 more source

CD4+ Tregs Drive Post‐Ischemic Sprouting Angiogenesis via Endothelial YY1/MAML1 Reactivation

Advanced Science, EarlyView.
ABSTRACT Microvascular complications of diabetes are chronic diseases of small vessels. We previously found that CD4+ regulatory T‐cells (Tregs) are markedly reduced in type 2 diabetes (T2D) after ischemic injury in both mice and humans, and that Treg deficiency in immunodeficient mice impairs vascular regeneration.
Hang Qu, Cheng Kiu Ho, Jitao Liu, Lei Cui, Yangfeng Hou, Cai Liang, Wenchu Ye, Randolph H. L. Wong, Shohei Hori, Bin Zhou, Kathy O. Lui   +10 more
wiley   +1 more source