Results 81 to 90 of about 93,955 (296)

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

CRSensor: A Synchronized and Impact-Aware Traceability Framework for Business Application Development

open access: yesApplied Sciences
To enable effective change impact management in business applications, robust requirements traceability is essential. However, manual approaches are inefficient and prone to errors. While the prior Model-Driven Engineering (MDE)-based research, including
Soojin Park
doaj   +1 more source

Traceability for Model Driven, Software Product Line Engineering [PDF]

open access: yes, 2008
Traceability is an important challenge for software organizations. This is true for traditional software development and even more so in new approaches that introduce more variety of artefacts such as Model Driven development or Software Product Lines ...
Anquetil, N.   +7 more
core   +5 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

A Lightweight Accountable Parallel Blockchain Architecture Based on Redactable Blockchain for Agri-Food Traceability

open access: yesFoods
Agri-food safety issues have received widespread attention globally. The emergence of blockchain technology (BCT) effectively addresses trust issues in the agri-food supply chain traceability system (AFSCTS).
Feng Chen   +4 more
doaj   +1 more source

A Study on the Effect of Traceability Links in Software Maintenance

open access: yesIEEE Access, 2013
In software development, stakeholders of the same project often collaborate asynchronously through shared artifacts. A traceability system links a project's artifacts and therefore provides support for collaboration among stakeholders.
Khaled Jaber, Bonita Sharif, Chang Liu
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Printed Electronics in Product Identification and Tracing [PDF]

open access: yes, 2009
Food and livestock traceability and tracking are now very hot topics because of severe threats to health and the need to comply with stringent new legislation. The traceability of products and components has received critical attention over the past few
Füzesi, István, Herdon, Miklós
core  

Safety-Critical Systems and Agile Development: A Mapping Study

open access: yes, 2018
In the last decades, agile methods had a huge impact on how software is developed. In many cases, this has led to significant benefits, such as quality and speed of software deliveries to customers.
Calikli, Gul   +4 more
core   +1 more source

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