Vitae, 2010 Se realizó un estudio comparativo de los métodos de resazurina y MTT como indicadores de medida indirecta de sobrevivencia celular, aplicables para la estimación de actividad citotóxica, con el fin de establecer la idoneidad de cada uno de los dos ...
Linamaría ESCOBAR M +2 more
doaj
On the enzymatic mechanism of 2-hydroxyisocaproyl-CoA dehydratase from Clostridium difficile [PDF]
, 2004 Die Gene ldhA und hadA aus Clostridium difficile (DSMZ 1296T) wurden kloniert und in Escherichia coli exprimiert. Die erhaltenen Proteine wurden gereinigt und als D-2-Hydroxyisocaproat-Dehydrogenase (LdhA) und 2-Hydroxyisocaproat-CoA-Transferase (HadA)
Buckel Wolfgang (Prof. Dr.), Kim Ji-Hoe
core +1 more source
Steerable isobutyric and butyric acid production from CO2 and H2 by Clostridium luticellarii
Microbial Biotechnology, Volume 17, Issue 1, January 2024.Clostridium luticellarii is a highly flexible microorganism, able to reverse its (reverse) beta‐oxidation pathway depending on the pH. At circumneutral pH, C. luticellarii produces acetic acid from H2 and CO2 but is able to co‐oxidize organic acids and isomerize butyric acid. At mildly acidic pH (~5.5), C.
Quinten Mariën +2 more
wiley +1 more source
COMPARISON OF RESAZURIN AND MTT METHODS ON STUDIES OF CITOTOXICITY IN HUMAN TUMOR CELL LINES
Vitae, 2010 A study was carried comparing the resazurina and MTT methods as indicators of indirect measure of cellular survival applied to valuated cytotoxic activity in order to assess the suitability of each of the two detection systems.
Linamaría ESCOBAR M. +2 more
doaj
Vitae, 2009 Several methodologies have been proposed in order to establish anticancer action, mainly focused on in vitro cytotoxicity valuation on neoplasic cell lines derived from human cancer.
Stephanie O. PRIETO +1 more
doaj
ATG5 is essential for ATG8-dependent autophagy and mitochondrial homeostasis in Leishmania major [PDF]
, 2012 Macroautophagy has been shown to be important for the cellular remodelling required for Leishmania differentiation. We now demonstrate that L. major contains a functional ATG12-ATG5 conjugation system, which is required for ATG8-dependent autophagosome ...
A Garami +61 more
core +4 more sources
Effect of chemotherapeutic drugs on caspase-3 activity, as a key biomarker for apoptosis in ovarian tumor cell cultured as monolayer : a pilot study [PDF]
, 2015 We aimed to develop a cost-effective and robust method to predict drug resistance in individual patients. Representative tissue fragments were obtained from tumors removed from female patients, aged 24-74 years old.
Gregoraszczuk, Ewa +4 more
core
Role of LpnA in Francisella tularensis Susceptibility to Resazurin
Proceedings of the West Virginia Academy of Science, 2023 Francisella tularensis is classified by the Centers for Disease Control and Prevention as a Category A bioterrorism agent due to its low infectious dose, ease of aerosolization, and high mortality rate. Given there is no licensed tularemia vaccine and the possibility of antibiotic-resistant F.
Emma Beatty, Deanna Schmitt
openaire +1 more source
Changes in miRNA Expression Profiling during Neuronal Differentiation and Methyl Mercury-Induced Toxicity in Human in Vitro Models [PDF]
, 2014 MicroRNAs (miRNAs) are implicated in the epigenetic regulation of several brain developmental processes, such as neurogenesis, neuronal differentiation, neurite outgrowth and synaptic plasticity.
BREMER Susanne +8 more
core +1 more source
Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay? [PDF]
Molecules, 2018 Resazurin (or Alamar Blue) is a poorly fluorescent dye. During the cellular reduction of resazurin, its highly fluorescent product resorufin is formed. Resazurin assay is a commonly applied method to investigate viability of bacterial and mammalian cells.
Rita Csepregi +6 more
openaire +2 more sources