Results 231 to 240 of about 654,709 (293)
FEBS Open Bio, EarlyView.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
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FEBS Open Bio, EarlyView.The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel +13 more
wiley +1 more source
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JONA: The Journal of Nursing Administration, 1982
Research design is the plan, structure, and strategy of investigation for answering the research question. The correct design helps isolate items of concern so that they can be examined under known conditions; it eliminates bias and reduces the margin of error, enabling the researcher to state confidently conclusions on which to base future decisions ...
Carol A. Lindeman, Donna Schantz
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Research design is the plan, structure, and strategy of investigation for answering the research question. The correct design helps isolate items of concern so that they can be examined under known conditions; it eliminates bias and reduces the margin of error, enabling the researcher to state confidently conclusions on which to base future decisions ...
Carol A. Lindeman, Donna Schantz
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Researching Design and Designing Research
Design Issues, 1999When Design Research began, say in the 1960s, the eventual success of science was assumed. Already, at the notorious 1956 Oxford Conference, architectural education in the UK (and its sphere of influence) accepted architecture was a second class subject: ie not properly scientific.
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Research into, by and for design
Journal of Visual Art Practice, 2008Mature theory construction in design research has been hampered by ill-considered ideas. The notion of research by design is such an idea, conflating practice and research in ways that make explicit theory development difficult. This article examines some of the problems associated with the notion of research by design.
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To Design Experimental Research
The American Journal of Nursing, 1962DURING THE PAST SEVERAL years, increasing emphasis has been placed on the need for nurses to examine nursing practice critically and scientifically. The conscientious and alert nurse is often concerned about the effect of a particular nursing practice, the relative effectiveness of different approaches, and numerous other questions which can be best ...
Irene M. Hulicka, Karel Hulicka
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