Results 311 to 320 of about 12,197,949 (364)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Reinforcing Primary Care in Korea: Policy Implications, Data Sources, and Research Methods. [PDF]

J Korean Med Sci
Kim CN, Yoon SJ.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Examining the research methods of early warning signals in clinical psychology through a theoretical lens. [PDF]

BMC Psychiatry
Cui J, Olthof M, Hasselman F, Lichtwarck-Aschoff A.   +3 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Outcomes of Foundational Learning in Research Methods Following Primary Medical Qualification on Surgical Research: A Retrospective Review. [PDF]

ANZ J Surg
Patel M, Sun D, Starr MJ, Solanki D, Upadhyay J, Edwards DJA, Raju A, Maouris T, Lombardo A, Wang D, Nagi K, Bhimani N, Glover AR.   +12 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Patients' and providers' evaluations of telepsychiatry and future directions: A literature review of mixed research methods. [PDF]

PCN Rep
Natsukari I, Ozaki N.
europepmc   +1 more source

Standardizing research methods for opioid dose comparison: the NIH HEAL morphine milligram equivalent calculator. [PDF]

Pain
Adams MCB, Sward KA, Perkins ML, Hurley RW.   +3 more
europepmc   +1 more source

Research and Improvement of Methods for Predicting Hidden Fracturing in a Rock Mass at a Polymetallic Mine

Nurbol BAKHTYBAYEV, О.А. Абиль, A.S. Bakhtybayeva   +2 more
openalex   +1 more source