Results 41 to 50 of about 32,576 (217)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Design of Multi-Functional Bio-Safe Dental Resin Composites with Mineralization and Anti-Biofilm Properties

Journal of Functional Biomaterials
This study aims to develop multi-functional bio-safe dental resin composites with capabilities for mineralization, high in vitro biocompatibility, and anti-biofilm properties. To address this issue, experimental resin composites consisting of UDMA/TEGDMA-
Jiaojiao Yun, Michael F. Burrow, Jukka P. Matinlinna, Hao Ding, Sin Man (Rosalind) Chan, James K. H. Tsoi, Yan Wang   +6 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Multiscale mechanical property prediction of unidirectional fiber-reinforced composites containing resin-deficient defects

Composites Part C: Open Access
A prediction model for mechanical property of composites with resin-deficient defects was established. Based on the homogenization theory, the characteristic parameters (volume fraction) of the resin-deficient defects were introduced into the Chamis ...
Mingxuan Li, Yaosheng Liang, Zhibo Duan, Xinwei Lin, Xiaolei Zhu   +4 more
doaj   +1 more source

Effect of Heterogeneous Resin Curing Agent on Micro-mechanical Uniformity of Resin Matrix of Composites

Cailiao gongcheng, 2018
Preparation of prepregs by hot melt method is generally involved the infiltration of resin. For the epoxy resin with a heterogeneous curing system, the preparation of large thickness prepregs may be affected by the uneven distribution of curing agent.
GUO Miao-cai, HONG Xu-hui, LI Ya-feng
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Comparison of polymerization behaviors, microhardness and compressive strength between bulk-fill resin and dual-cured core resin

BMC Oral Health
Objectives This study aimed to investigate the polymerization shrinkage, microhardness, DOC, and compressive strength of composite resin materials employed for core restoration, encompassing dual-cured core resin composites and LC bulk-fill composites ...
Hye Jeong Kim, Jiyoung Kwon, Hyun-Jung Kim, Reuben H. Kim, Duck-Su Kim, Ji-Hyun Jang   +5 more
doaj   +1 more source

Fiber reinforced silicon-containing arylacetylene resin composites

eXPRESS Polymer Letters, 2007
A silicon-containing arylacetylene resin (SAR), a poly(dimethylsilyleneethynylene phenyleneethynylene) (PMSEPE), was synthesized. The PMSEPE is a solid resin at ambient temperature with a softening temperature about 60°C and soluble in some solvents like
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source