Results 61 to 70 of about 152,310 (267)

A novel strategy combining full Fourier transform infrared spectroscopy with machine learning enhanced the accuracy for analyzing cell wall polymers in maize stover

open access: yesIndustrial Crops and Products
Cellulose, hemicellulose and lignin are three major cell wall polymers extracted from crop feedstocks to produce valuable biofuels and bio-products. Due to the labor-intensive and environmentally hazardous characteristics of chemical methods for plant ...
Zihao Gui   +10 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

open access: yesFEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner   +7 more
wiley   +1 more source

The Maize NBS-LRR Gene ZmNBS25 Enhances Disease Resistance in Rice and Arabidopsis

open access: yesFrontiers in Plant Science, 2018
Nucleotide-binding site-leucine-rich repeat (NBS-LRR) domain proteins are immune sensors and play critical roles in plant disease resistance. In this study, we cloned and characterized a novel NBS-LRR gene ZmNBS25 in maize.
Yunjian Xu   +7 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

open access: yesFEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay   +2 more
wiley   +1 more source

How phagocytic cells kill bacteria: Lessons from a professional killer

open access: yesFEBS Open Bio, EarlyView.
How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley   +1 more source

OsPUP1 Modulates Cytokinin Distribution and Antioxidant Defense to Regulate Heat Stress Tolerance in Rice

open access: yesAntioxidants
Heat stress has emerged as one of the major environmental factors constraining rice growth. In our previous studies, OsPUP1, a member of the purine transporter protein family in rice, has been verified to be involved in the transport of cytokinin (CK ...
Shujie Wang   +11 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

The Mitochondrial Protein RESISTANCE to APHIDS 9 Interacts with S40 to Resist Aphid Infestation by Modulating Reactive Oxygen Species Homeostasis in Maize (Zea mays)

open access: yesAdvanced Science
As the corn aphid (Rhopalosiphum maidis) poses a major threat to maize (Zea mays) growth, there is much interest in identifying aphid resistance genes. In this study, an aphid‐susceptible maize mutant from an ethyl methanesulfonate–mutagenized library is
Chuanhong Wang   +12 more
doaj   +1 more source

The ZmHSF08-ZmUGT92A1 module regulates heat tolerance by altering reactive oxygen species levels in maize

open access: yesCrop Journal
GTs (Glycosyltransferases) are important in plant growth and abiotic stresses. However, its role in maize heat response is far from clear. Here, we describe the constitutively expressed UDP-glycosyltransferase ZmUGT92A1, which has a highly conserved PSPG
Hao Li   +6 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

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