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Validating Flow-through Respirometry

2008
This chapter describes methods for validating the measurements made using flow-through respirometry. These methods include the injection of nitrogen at a known flow rate into a respirometry system; burning a small flame of ethanol or methanol at a rate measured by weighing the lamp (often called “alcohol recovery”); and burning hydrocarbon gases such ...
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High-Resolution Respirometry in a Small-Volume Chamber

Journal of Visualized Experiments
Investigating respiratory fluxes is decisive for understanding the complex interplay between metabolic processes. Studies of cells and tissues with limited sample availability and low respiratory rates may benefit from small experimental volumes. We examined if the 0.5 mL chamber yields results consistent with the 2.0 mL chamber at identical sample ...
Leo, Elettra   +5 more
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Calculation of ventilation threshold using noncontact respirometry

2008 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2008
We proposed the calculation method of the ventilation threshold using the noncontact respirometry under pedal stroke motion. By the simultaneous measurement with the expiration gas analyzer, we examined the effectiveness of the proposal method. There was high correlation over 0.8 between ventilation variations calculated by our proposed method and the ...
Hirooki, Aoki   +3 more
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[Examination of liver mitochondria with respirometry].

Magyar sebeszet, 2017
Due to their diverse physiological functions, mitochondria can cause various acute and chronic liver diseases, thus being potential targets for therapies and diagnostics as well. In this study, the advantages of high-resolution respirometry are presented for the assessment of liver mitochondrial functions.
Gerda, Strifler   +6 more
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Capacitance Respirometry

Archives of Environmental Health: An International Journal, 1969
R E, Barrow, A J, Vorwald, E, Domeier
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Flow-through Respirometry: The Basics

2018
This chapter describes the basic theory behind the most widely used method for measuring metabolic rates: flow-through or open-system respirometry. The advantages and disadvantages of the technique are summarized and the two major types of flow-through respirometry systems are described. Recommendations are given on choosing an appropriate flow rate to
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Dynamic respirometry of division synchronized Astasia longa

Experimental Cell Research, 1965
Abstract A dynamic respirometer has been developed and used to measure the respiration rates of temperature-synchronized Astasia longa. The instrument measures the respiration rate of cells in the synchronizing apparatus by evaluating the diffusion rate into the flask as a function of oxygen tension in the culture medium.
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High-Resolution Respirometry for the Assessment of Teratogenic Chemicals

Pesticides are often used in agriculture and residential areas to mitigate pests and weeds. These chemicals can enter aquatic ecosystems via runoff and rain events, exerting negative effects on aquatic species. In rapidly developing fish embryos, metabolic disruption can alter the developmental trajectory and alter ATP levels.
Christopher L. Souders   +2 more
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Room Calorimetry and Mask Respirometry

2018
This chapter describes two approaches to human metabolic measurement: room calorimetry and dilution mode mask (or hood, or canopy) respirometry. For room calorimetry, where the EE of a subject is typically monitored for 24 h, strategies for arranging air flow systems and the convective air movement required for effective time constant compensation are ...
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Flow-through Respirometry: Incurrent Flow Measurement

2018
This chapter describes the setup, plumbing, and equations required for applying a respirometry system wherein the flow rate of the air entering the animal chamber is known. Such systems are usually referred to as push systems, because the air is usually pushed into a sealed respirometer chamber at a known rate, and the concentrations of incurrent and ...
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