Results 81 to 90 of about 12,674,101 (309)

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Resting-state functional connectivity of orthographic networks in acquired dysgraphia

Frontiers in Psychology, 2015
Introduction. While task-based fMRI has been used extensively to study the functional neural networks that instantiate cognitive functions, there is increasing interest in analyzing the activity of the brain while it is not performing a task – in the ...
Gali Ellenblum
doaj   +1 more source

Resting-state brain entropy in schizophrenia

Comprehensive Psychiatry, 2019
The human brain presents ongoing temporal fluctuations whose dynamic range indicates the capacity of information processing and can be approximately quantified with entropy. Using functional magnetic resonance imaging (fMRI), recent studies have shown a stable distribution pattern of temporal brain entropy (tBEN) in healthy subjects, which may be ...
Shao-Wei Xue, Qingbao Yu, Yonghu Guo, Donghui Song, Ze Wang   +4 more
openaire   +3 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Resting-state electroencephalographic functional network alterations in major depressive disorder following magnetic seizure therapy

, 2021
Resting-state electroencephalographic functional network alterations in major depressive disorder following magnetic seizure ...
Aron Hill (13059099), F Farzan (13479364), I Hadas (13447248), R Zomorrodi (13323645), D Voineskos (13447251), ZJ Daskalakis (14454471), PB Fitzgerald (14451639), DM Blumberger (14454456), A Throop (13482853)   +8 more
core  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Resting-state and vocabulary tasks distinctively inform on age-related differences in the functional brain connectome

, 2019
Most of the current knowledge about age-related differences in brain neurofunctional organisation stems from neuroimaging studies using either a “resting state” paradigm, or cognitive tasks for which performance decreases with age. However, it remains to
Jason Steffener, Stern, Yaakov, Yves Joanette, Yaakov Stern, Benhajali, Yassine, Yassine Benhajali (624938), Jason Steffener (135960), Yves Joanette (5834885), Joanette, Yves, Perrine Ferré (5834882), Yaakov Stern (135965), Perrine Ferré, Pierre Bellec (279622), Yassine Benhajali, Steffener, Jason, Bellec, Pierre, Ferré, Perrine, Pierre Bellec   +17 more
core   +1 more source

Resting-state functional connectivity alterations after mild COVID-19 infection

BMC Infectious Diseases
The long-term effects of coronavirus disease 2019 (COVID-19) are more pronounced after severe infection, although years after the pandemic was declared, long-term neurological complications after mild infection have begun to unfold.
Anna Szente, Ákos Arató, Husamalddin Ali Alhour, Gábor Perlaki, Gergely Orsi, Szilvia Anett Nagy, Eszter Áfra, Barnabás Dudás, Dalma Tényi, Péter Barsi, Norbert Kovács, József Janszky, Gergely Darnai   +12 more
doaj   +1 more source