Results 111 to 120 of about 3,197,803 (398)

Polimorfismo no gene GH1-PstI associado a características corporais de linhagens de tilápia-do-nilo Polymorphism in the GH1-PstI gene associated to corporal characteristics in Nile tilapia strains

open access: yesPesquisa Agropecuária Brasileira, 2009
O objetivo deste trabalho foi determinar a associação de polimorfismos no gene do hormônio crescimento GH1 às características corporais, em linhagens de tilápia-do-nilo (Oreochromis niloticus). Foram coletados fragmentos da nadadeira caudal de exemplares
Danielly Veloso Blanck   +3 more
doaj   +1 more source

Conflicts Targeting Epigenetic Systems and Their Resolution by Cell Death: Novel Concepts for Methyl-Specific and Other Restriction Systems [PDF]

open access: yes
Epigenetic modification of genomic DNA by methylation is important for defining the epigenome and the transcriptome in eukaryotes as well as in prokaryotes.
Aertsen   +107 more
core   +3 more sources

Type I restriction endonucleases are true catalytic enzymes [PDF]

open access: yes, 2009
Type I restriction endonucleases are intriguing, multifunctional complexes that restrict DNA randomly, at sites distant from the target sequence. Restriction at distant sites is facilitated by ATP hydrolysis-dependent, translocation of double-stranded ...
Berge   +42 more
core   +2 more sources

Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms

open access: yesFEBS Letters, EarlyView.
Mycobacterium tuberculosis exhibits a remarkable resilience to acid stress. In this Review, we discuss some of the molecular mechanisms and metabolic pathways used by the tubercle bacilli to adapt and resist host‐mediated acid stress. Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and ...
Janïs Laudouze   +3 more
wiley   +1 more source

Enzymatic Digestion Pattern of Varicella Zoster Virus ORF38 and ORF54 in Chickenpox Patients Using RFLP Technique [PDF]

open access: yesIranian Journal of Pathology, 2016
Background: Varicella zoster virus (VZV) causes chickenpox in children and zoster (zona) in the elderly. Using RFLP-PCR method for detection of VZV specific SNPs ORF38, 54 and 62 could  distinguish the profile of VZV isolates.
Hossein Keyvani   +2 more
doaj  

DNA Methylation Tools and Strategies: Methods in a Review

open access: yesAsian Pacific Journal of Cancer Biology, 2019
DNA methylation is known as an important epigenetic change in plants and vertebrates genome. In this process, the methyl group transferred by DNA methyl transferase enzymes to cytosine at carbon residue 5 often in the CpG dinucleotide context.
Mohammad Hadi Karbalaie Niya   +2 more
doaj   +1 more source

Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns [PDF]

open access: yes, 1933
Hepatitis B virus (HBV) is classified into genotypes A–F, which is important for clinical and etiological investigations. To establish a simple genotyping method, 68 full-genomic sequences and 106 S gene sequences were analyzed by the molecular ...
Mizokami, Masashi   +6 more
core   +2 more sources

Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.

open access: yesNucleic Acids Research, 1999
We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation ...
D. Tillett, B. Neilan
semanticscholar   +1 more source

Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis

open access: yesFEBS Letters, EarlyView.
The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Laetitia Gilet   +4 more
wiley   +1 more source

Identification of extracellular enzyme producing alkalophilic bacilli from Izmir province by 16S-ITS rDNA RFLP [PDF]

open access: yes, 2004
Aims: To screen industrially important extracellular enzymes from the newly isolated alkalophilic bacilli and to characterize them by phenotypic and 16S-internal transcribed spacer (ITS) rDNA restriction pattern analysis.
Akbalık, Güney   +6 more
core   +1 more source

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