Results 121 to 130 of about 3,197,803 (398)

Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes.

Proceedings of the National Academy of Sciences of the United States of America, 1983
Three sets of cosmid clones--containing the HLA-DR alpha chain gene and two additional related genes--were isolated from human genomic DNA libraries by using a cDNA probe for the HLA-DR alpha chain.
J. Trowsdale, J. Lee, J. Carey, F. Grosveld, J. Bodmer, W. Bodmer   +5 more
semanticscholar   +1 more source

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

FEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma, Jennifer R. Deuis, Xinying Jia, Theo Crawford, Sassan Rahnama, Eivind A. B. Undheim, Irina Vetter, Yanni K. ‐Y. Chin, Mehdi Mobli   +8 more
wiley   +1 more source

The power of microRNA regulation—insights into immunity and metabolism

FEBS Letters, EarlyView.
MicroRNAs are emerging as crucial regulators at the intersection of metabolism and immunity. This review examines how miRNAs coordinate glucose and lipid metabolism while simultaneously modulating T‐cell development and immune responses. Moreover, it highlights how cutting‐edge artificial intelligence applications can identify miRNA biomarkers ...
Stefania Oliveto, Nicola Manfrini, Stefano Biffo   +2 more
wiley   +1 more source

Advantages of using the QIAshredder instead of restriction digestion to prepare DNA for droplet digital PCR

BioTechniques, 2014
The viscosity of genomic DNA can interfere with digital PCR systems that partition samples into oil droplets or microfluidic wells. Restriction digestion may reduce the viscosity, but the process is labor-intensive, and the buffer can alter the ...
Steven A. Yukl, Philipp Kaiser, Peggy Kim, Peilin Li, Joseph K. Wong   +4 more
doaj   +1 more source

Identification of novel small molecule inhibitors of ETS transcription factors

FEBS Letters, EarlyView.
ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla, Zary Forghany, Jin Ma, Johan G. Hollander, Ruta Nachane, Karoly Szuhai, Pancras C. W. Hogendoorn, Peter ten Dijke, Dipen Shah, David A. Baker   +9 more
wiley   +1 more source

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

Molecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever, Renske D. M. Steenbergen   +1 more
wiley   +1 more source

Subtracted Restriction Fingerprinting—A Tool for Bacterial Genome Typing

BioTechniques, 2003
Reproducible, discriminative, high-throughput methods are required for the identification of bacterial strains and isolates in a clinical environment. A new molecular typing method for bacteria was developed and tested on Salmonella and E.
Valeri Terletski, Stefan Schwarz, Joseph Carnwath, Heiner Niemann   +3 more
doaj   +1 more source

Fused eco29kIR- and M genes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems [PDF]

, 2011
Background The discovery of restriction endonucleases and modification DNA methyltransferases, key instruments of genetic engineering, opened a new era of molecular biology through development of the recombinant DNA technology.
A Karyagina, A Pertzev, A Piekarowicz, Alexander S Solonin, D Nikitin, D Nikitin, Dmitri V Nikitin, E Cesnaviciene, E Ibryashkina, E Zaitzev, G Gemmen, GG Wilson, H Kong, I Kobayashi, J Bujnicki, J Nakonieczna, J Sambrook, J Vitor, K Alexandrov, K Lepikhov, K Lunnen, KJ Skowronek, L Bougueleret, M Denjmukhametov, M Ibryashkina, M Nagornykh, M Zakharova, M Zakharova, Marina L Mokrishcheva, R Mushtaq, R Rimseliene, R Rubin, RJ Roberts, RJ Roberts, RJ Williams, S Jurenaite-Urbanaviciene, S Jurenaite-Urbanaviciene, U Laemmli   +37 more
core   +3 more sources

The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization [PDF]

, 2016
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
arxiv   +1 more source

Apolipoprotein E polymorphism determined by restriction enzyme analysis of DNA amplified by polymerase chain reaction: convenient alternative to phenotyping by isoelectric focusing.

Clinical Chemistry, 1990
Three common alleles determine six apolipoprotein E (apo E) phenotypes that are associated with variations in serum cholesterol in the population. This genetic variation results from single nucleotide alterations at two DNA loci encoding the amino acid ...
Klmmo Kontula, Katriina Aaito-Set#{225}l, Timo Kuusi, Lilsa H#{228}m#{228}l#{228}lnen, A. Syvanen   +4 more
semanticscholar   +1 more source