Results 331 to 340 of about 647,244 (359)
Restriction Enzyme Computation [PDF]
, 2003 In this paper implementation of an inner hair cell model, including macromechanics and mechanical to neural transduction process is presented and discussed. The well-known Meddis model will be use as the reference system, and a high level synthesis will provide a parametrizable implementation and a reusability code, which allows future refinements on ...
Maciej Troc, Olgierd Unold
openaire +1 more source
Engineered Calcium-Precipitable Restriction Enzyme [PDF]
ACS Synthetic Biology, 2014 We have developed a simple system for tagging and purifying proteins. Recent experiments have demonstrated that RTX (Repeat in Toxin) motifs from the adenylate cyclase toxin gene (CyaA) of B. pertussis undergo a conformational change upon binding calcium, resulting in precipitation of fused proteins and making this method a viable alternative for ...
Timothy Read +9 more
openaire +2 more sources
Some of the next articles are maybe not open access.
Related searches:
Related searches:
Class-IIS restriction enzymes — a review
Gene, 1991Class-IIS restriction enzymes (ENases-IIS) interact with two discrete sites on double-stranded DNA: the recognition site, which is 4-7 bp long, and the cleavage site, usually 1-20 bp away from the recognition site. The recognition sequences of ENases-IIS are totally (or partially) asymmetric and all of the characterized ENases-IIS are monomeric.
Szybalski, W +3 more
openaire +4 more sources
ATP-dependent restriction enzymes
2000The phenomenon of restriction and modification (R-M) was first observed in the course of studies on bacteriophages in the early 1950s. It was only in the 1960s that work of Arber and colleagues provided a molecular explanation for the host specificity.
Rao, Desirazu N +2 more
openaire +3 more sources
1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
openaire +2 more sources
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
openaire +2 more sources
Model for wandering restriction enzymes
Nature, 1974I WISH to propose a model for those DNA restriction enzymes which, although requiring specific recognition sites, make a limited number of cuts at apparently random sites, far from the recognition sites.
openaire +3 more sources
[21] Restriction enzymes: Properties and use
1992Publisher Summary This chapter lists all commercially available enzymes, to describe their general properties, the basic procedures for their use, and ways of troubleshooting problems encountered in their use. Although the number of enzymes being discussed is large, a few simple rules apply to virtually all the enzymes.
openaire +3 more sources
Understanding Restriction Enzyme Digests
2021Prior to submitting genomic DNA for sequencing, SEA students perform a restriction endonuclease digest with several enzymes, which provides a fast and cost-effective way of quickly screening isolated phages. The pattern of bands on the gel should be unique except for very closely related or identical phages, which is useful when trying to identify ...
Dustin Edwards +4 more
openaire +2 more sources
Caloric restriction, aging, and antioxidant enzymes
Mutation Research/DNAging, 1993The basic mechanisms of aging and its retardation by caloric restriction (CR) remain unclear. One suggested means by which CR could retard aging is based on production of mitochondrial free radicals, and efficiency of their subsequent metabolism. Currently, there is little information concerning the influences of age and CR on the rates of in vivo ...
Ronald W. Hart +2 more
openaire +3 more sources
High Throughput Cloning with Restriction Enzymes
2008The systematic structural analysis of many target proteins involves generating expression clones in high throughput. This requires robust laboratory procedures and benefits from laboratory automation and data management systems. This chapter gives an overview of the Protein Structure Factory, a structural genomics project focusing on human proteins ...
Asgar Ergin +2 more
openaire +3 more sources