Julius-Kühn-Archiv, 2020 Hühnerhirse (Echinochloa crus-galli) gewinnt als Ungras im Maisanbau zunehmend an Bedeutung. Einseitiger Einsatz von ALS-Inhibitoren erzeugt einen Selektionsdruck, der zu vermehrtem Auftreten von Resistenzen führt.
Runge, Fabian +4 more
doaj +1 more source
DNA Cloning without Restriction Enzyme and Ligase
BioTechniques, 1999 One common problem in using the traditional DNA cloning procedure is that suitable natural restriction sites are often unavailable for a given task. Creating new restriction sites is often time consuming. Here, I describe a simple technique of producing “
Hung Tseng
doaj +1 more source
The Quasi-Steady-State Approximations revisited: Timescales, small parameters, singularities, and normal forms in enzyme kinetic [PDF]
Mathematical Biosciences, Volume 325, July 2020, 108339, 2019 In this work, we revisit the scaling analysis and commonly accepted conditions for the validity of the standard, reverse and total quasi-steady-state approximations through the lens of dimensional Tikhonov-Fenichel parameters and their respective critical manifolds.
arxiv +1 more source
Nucleic Acids Research, 2009 Atomic force microscopy (AFM) allows the study of single protein–DNA interactions such as those observed with the Type I Restriction–Modification systems.
Kelly J. Neaves +6 more
semanticscholar +1 more source
Removal of a frameshift between the hsdM and hsdS genes of the EcoKI Type IA DNA restriction and modification system produces a new type of system and links the different families of Type I systems [PDF]
, 2012 The EcoKI DNA methyltransferase is a trimeric protein comprised of two modification subunits (M) and one sequence specificity subunit (S). This enzyme forms the core of the EcoKI restriction/modification (RM) enzyme. The 3′ end of the gene encoding the M
Blakely, Garry W +5 more
core +1 more source
APOBEC3-mediated restriction of RNA virus replication
Scientific Reports, 2018 APOBEC3 family members are cytidine deaminases with roles in intrinsic responses to infection by retroviruses and retrotransposons, and in the control of other DNA viruses, such as herpesviruses, parvoviruses and hepatitis B virus.
A. Milewska +7 more
semanticscholar +1 more source
A model of dynamic stability of H3K9me3 heterochromatin to explain the resistance to reprogramming of differentiated cells [PDF]
Biochim Biophys Acta. Gene Regulatory Mechanisms 2017
Feb;1860(2):184-195, 2017 Despite their dynamic nature, certain chromatin marks must be maintained over the long term. This is particulary true for histone 3 lysine 9 (H3K9) trimethylation, that is involved in the maintenance of healthy differentiated cellular states by preventing inappropriate gene expression, and has been recently identified as the most efficient barrier to ...
arxiv +1 more source
Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)
PeerJ, 2019 Molecular ecologists frequently use genome reduction strategies that rely upon restriction enzyme digestion of genomic DNA to sample consistent portions of the genome from many individuals (e.g., RADseq, GBS). However, researchers often find the existing
N. Bayona‐Vásquez +12 more
semanticscholar +1 more source
Identifikasi polimorfisme gen GH (Growth Hormone) sapi Bali dengan metode PCR-RFLP
Journal of Biological Researches, 2012 This study was conducted to identify polymorphism of growth hormone gene of Bali cattle. A PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) procedure was developed for determining polymorphism of growth hormone gene.
Sutiman Bambang Sumitro +3 more
doaj +1 more source
Computer Aided Simulation of DNA Fingerprint Amplified Fragment Length Polymophism (AFLP) Using Suffix Tree Indexing and Data Mining [PDF]
, 2011 AFLP is one of the DNA Fingerprinting techniques which have broad application as genetic marker in various fields. Begin with the DNA sequence digestion using one or more particular restriction enzyme, ligation of the adapters to the overhanging sticky ...
Budiman, Agung +3 more
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