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Restriction Fragment Length Polymorphism Typing of Mycobacteria
2003In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS6110 RFLP typing of Mycobacterium tuberculosis complex isolates (1) and IS1245 RFLP typing of Mycobacterium ...
van Soolingen, Drivm+2 more
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Chromosome 13 restriction fragment length polymorphisms
Human Genetics, 1984The gene locus for hereditary retinoblastoma is on human chromosome 13, band q14. With this gene localization in mind, we cloned DNA fragments from this chromosome. Three of the fragments identify restriction fragment length polymorphisms. These three fragments are from the region 13q12-13q22, the chromosome region which contains the retinoblastoma ...
G. A. P. Bruns+3 more
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Restriction fragment length polymorphism [PDF]
In the 1960s, a group of enzymes were discovered in bacteria that could degrade incoming bacteriophage DNA and would ‘restrict’ their establishment in the cell [2,6]. These enzymes, known as restriction enzymes [19], have proved very valuable in modern manipulations of DNA.
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Definition of DRw10 by restriction fragment length polymorphism
Tissue Antigens, 1989To better define the presence of the DRw10 haplotype which has sometimes proved difficult to type by using serologic reagents, Southern blot analysis was performed on seven DRw10 heterozygous individuals with rheumatoid arthritis. Using the restriction enzymes Taq I or BamH I, the restriction fragment length polymorphism (RFLP) pattern for the DRw10 ...
Antonio Núñez-Roldán+4 more
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Restriction Fragment Length Polymorphism Analysis
Current Protocols in Human Genetics, 1994AbstractThis unit describes Restriction Fragment Length Polymorphism (RFLP) analysis, which utilizes restriction endonuclease digestion to identify DNA sequence polymorphisms in genes or DNA regions of interest. When investigating families for inheritance of an RFLP, aliquots of genomic DNA from individual family members are digested to completion with
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Restriction Fragment Length Polymorphism of the D1S1 Locus in Italy
Human Heredity, 1988Three Italian populations were examined for a restriction fragment length polymorphism probing with a DNA sequence of unknown function located on chromosome 1. No difference was observed between the samples. The allele frequencies in Italy were: D1S1 BS = 0.82, D1S1 BF = 0.18.
Palmarino R, Mantuano E, Lucarelli P
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Restriction Fragment Length Polymorphism of the D5S4 Locus in Italy
Human Heredity, 1990Five Italian samples were examined for an EcoRI restriction fragment length polymorphism associated with a DNA sequence of unknown function, located on chromosome 5. No significant difference was observed between the samples. The allele frequencies in Italy were D5S4ES = 0.697, D5S4EF = 0.303.
Palmarino R+3 more
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Length polymorphisms of restriction fragments of rat mitochondrial DNAs
Biochemical and Biophysical Research Communications, 1981Abstract Differences were found in the HpaII cleavage patterns of two types of rat ( Rattus norvegicus ) mtDNA, types A and B. One HpaII fragment, Hpa5, of type A was about 30 base pairs smaller than that of type B, but no 30-base pair fragment was detectable in an HpaII digest of type A mtDNA.
Jun-Ichi Hayashi+2 more
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Restriction fragment length polymorphism analysis in ankylosing spondylitis
The American Journal of Medicine, 1988We utilized the technique of restriction fragment length polymorphism (RFLP) analysis in order to examine class I major histocompatibility complex genes in 52 Alabama ankylosing spondylitis patients and 107 local control subjects. A 9.2-kilobase PvuII RFLP was identified using the class I-specific B7 cDNA probe pDP001 that was closely associated with ...
Ronald T. Acton+4 more
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Linkage of restriction fragment length polymorphisms and isozymes in Citrus
Theoretical and Applied Genetics, 1992Genetic linkage analysis was performed using two segregating populations of citrus. One population arose from an intergeneric backcross of Citrus grandis (L.) Osb. cv 'Thong Dee' and Poncirus trifoliata (L.) Raf. cv 'Pomeroy', using the former as the recurrent (female) parent. The other population came from an interspecific backcross of C.
P. C. Liou+3 more
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