Results 91 to 100 of about 2,423,969 (313)

Assessment of Intraspecific Variation in Clavispora sp. by Restriction Mapping of Tandemly Repeated Deoxyribonucleic Acid

, 1985
The total deoxyribonucleic acids from 10 strains of an undescribed cactophilic species of Clavispora were subjected to restriction analyses with endonucleases ApaI, EcoRI, KpnI, and XholI. A 7.6-kilobase tandemly repeated segment of the genome was mapped
M. Lachance, Alexander Luttikhuis, L. L. Anweiler, Dascha L. Paylor, Lorraine G. Olson   +4 more
semanticscholar   +1 more source

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

AGORA: Assembly Guided by Optical Restriction Alignment

BMC Bioinformatics, 2012
Background Genome assembly is difficult due to repeated sequences within the genome, which create ambiguities and cause the final assembly to be broken up into many separate sequences (contigs).
Lin Henry C, Goldstein Steve, Mendelowitz Lee, Zhou Shiguo, Wetzel Joshua, Schwartz David C, Pop Mihai   +6 more
doaj   +1 more source

Decoding the dual role of autophagy in cancer through transcriptional and epigenetic regulation

FEBS Letters, EarlyView.
Transcriptional and epigenetic regulation controls autophagy, which exerts context‐dependent effects on cancer: Autophagy suppresses tumorigenesis by maintaining cellular homeostasis or promotes tumor progression by supporting survival under stress. In this “In a Nutshell” article, we explore the intricate mechanisms of the dual function of autophagy ...
Young Suk Yu, Ik Soo Kim, Sung Hee Baek
wiley   +1 more source

ICRPfinder: a fast pattern design algorithm for coding sequences and its application in finding potential restriction enzyme recognition sites

BMC Bioinformatics, 2009
Background Restriction enzymes can produce easily definable segments from DNA sequences by using a variety of cut patterns. There are, however, no software tools that can aid in gene building -- that is, modifying wild-type DNA sequences to express the ...
Stafford Phillip, Zhang Xiangmin, Li Yuhua, Li Chao, Dinu Valentin   +4 more
doaj   +1 more source

Autophagy in cancer and protein conformational disorders

FEBS Letters, EarlyView.
Autophagy plays a crucial role in numerous biological processes, including protein and organelle quality control, development, immunity, and metabolism. Hence, dysregulation or mutations in autophagy‐related genes have been implicated in a wide range of human diseases.
Sergio Attanasio
wiley   +1 more source

Mapping crossover events of mouse meiotic recombination by restriction fragment ligation-based Refresh-seq

Cell Discovery
Single-cell whole-genome sequencing methods have undergone great improvements over the past decade. However, allele dropout, which means the inability to detect both alleles simultaneously in an individual diploid cell, largely restricts the application ...
Yan Wang, Yijun Chen, Junpeng Gao, Haoling Xie, Yuqing Guo, Jingwei Yang, Jun’e Liu, Zonggui Chen, Qingqing Li, Mengyao Li, Jie Ren, Lu Wen, Fuchou Tang   +12 more
doaj   +1 more source

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source

A Dense Linkage Map of Lake Victoria Cichlids Improved the Pundamilia Genome Assembly and Revealed a Major QTL for Sex-Determination

G3: Genes, Genomes, Genetics, 2018
Genetic linkage maps are essential for comparative genomics, high quality genome sequence assembly and fine scale quantitative trait locus (QTL) mapping.
Philine G. D. Feulner, Julia Schwarzer, Marcel P. Haesler, Joana I. Meier, Ole Seehausen   +4 more
doaj   +1 more source