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Finding restriction sites [PDF]
Genome Biology, 2000 Webcutter provides a free and easy-to-use interface for the analysis of restriction sites in any sequence.
Mar Albà
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The cleavage site of the restriction endonuclease Ava II [PDF]
Nucleic Acids Research, 1978 We have determined that the type II restriction enzyme Ava II, isolated from Anabaena variabilis, recognizes and cuts the sequence (formula: see article). The eight Ava II sites of pBR322 have been mapped, as well as a unique site for Ava I.
Sutcliffe, JG, Church, George
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Re-evaluating the Systematics of Dendrolycopodium Using Restriction-Site Associated DNA-Sequencing [PDF]
Frontiers in Plant Science, 2022 The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or inconsistent characters.
Alaina R. Petlewski +8 more
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Restriction Site-Free Insertion of PCR Products Directionally into Vectors [PDF]
BioTechniques, 2000 A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions.
G.J. Chen +4 more
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PHYLOGENIES FROM RESTRICTION SITES: A MAXIMUM‐LIKELIHOOD APPROACH [PDF]
Evolution, 1992 Restriction sites data can be analyzed by maximum likelihood to obtain estimates of phylogenies. The likelihood methods of Smouse and Li, who were able to compute likelihoods for up to four species under a simplified model of base change, can be extended numerically to deal with any number of species. The computational methods for doing so are outlined.
Joseph Felsenstein
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Molecular Mapping of Restriction-Site Associated DNA Markers In Allotetraploid Upland Cotton. [PDF]
PLoS ONE, 2015 Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation
Yangkun Wang +8 more
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Frontiers in Plant Science, 2021 Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR.
Ruslan Kalendar +4 more
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REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer
SoftwareX, 2021 Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD +3 more
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Complete genome assembly and methylome dissection of Methanococcus aeolicus PL15/Hp
Frontiers in Microbiology, 2023 Although restriction-modification systems are found in both Eubacterial and Archaeal kingdoms, comparatively less is known about patterns of DNA methylation and genome defense systems in archaea.
Alexey Fomenkov +4 more
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Selective Protection of Restriction Endonuclease Sites [PDF]
BioTechniques, 2003 A difficulty that is encountered when attempting to insert a PCR-amplified product or DNA fragment of interest into a particular vector is the presence within the insert of one or more internal restriction endonuclease (RE) sites identical to those selected for the flanks of the insert.
Kagnew H. Gebreyesus, Roland A. Owens
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