Inverse Restriction Site-Associated DNA Sequencing (iRAD-seq) [PDF]
Bio-ProtocolReduced representation sequencing (RRS), particularly through restriction site-associated DNA sequencing (RAD-seq), has been widely adopted for whole-genome genotyping due to its cost-effectiveness and cross-species applicability.
Peng Chen +5 more
doaj +2 more sources
Re-evaluating the Systematics of Dendrolycopodium Using Restriction-Site Associated DNA-Sequencing [PDF]
Frontiers in Plant Science, 2022 The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or inconsistent characters.
Alaina R. Petlewski +8 more
doaj +2 more sources
Molecular Mapping of Restriction-Site Associated DNA Markers In Allotetraploid Upland Cotton. [PDF]
PLoS ONE, 2015 Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation
Yangkun Wang +8 more
doaj +2 more sources
Frontiers in Plant Science, 2021 Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR.
Ruslan Kalendar +4 more
doaj +1 more source
REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer
SoftwareX, 2021 Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD +3 more
doaj +1 more source
Complete genome assembly and methylome dissection of Methanococcus aeolicus PL15/Hp
Frontiers in Microbiology, 2023 Although restriction-modification systems are found in both Eubacterial and Archaeal kingdoms, comparatively less is known about patterns of DNA methylation and genome defense systems in archaea.
Alexey Fomenkov +4 more
doaj +1 more source
The Genetic Characterization of DGAT1 Gene in Donkey Populations Reared in Thrace Region of Turkey
Journal of Agricultural Sciences, 2020 AcylCoA: diacylglycerol acyltransferase (DGAT1) gene has a considerable effect on milk content and yield in cattle with a substitution of lysine by alanine in the exon 8 of the gene.
Fulya Özdil
doaj +1 more source
BBF RFC #111: Inducible expression vector with His tag fusion for purification of RFC25 parts [PDF]
, 2015 This Request For Comments (RFC) describes a new expression vector based on the pET expression system and designed for BioBrick RFC25 parts. This design includes all the features of pET expression vectors with a new origin of replication, resistance ...
Husser, Mathieu +2 more
core +15 more sources
A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus. [PDF]
PLoS ONE, 2014 PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion.
Maria W Smith +5 more
doaj +1 more source
indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events. [PDF]
PLoS ONE, 2017 Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations
Charles Hodgens +2 more
doaj +1 more source