Results 91 to 100 of about 1,167,532 (299)

Optimizing Restriction Site Placement for Synthetic Genomes

Information and Computation, 2010
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Montes, Pablo, Memelli, Heraldo, Ward, Charles B., Kim, Joondong, Mitchell, Joseph S.B., Skiena, Steven   +5 more
openaire   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments

BioTechniques, 2005
Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report
Ulrich Krauss, Thorsten Eggert
doaj   +1 more source

Bat IFITM3 restriction depends on S-palmitoylation and a polymorphic site within the CD225 domain

Life Science Alliance, 2020
Comparative analysis reveals adaptive evolution of bat IFITMs and a naturally polymorphic site within the conserved CD225 domain that affects S-palmitoylation and antiviral function of bat IFITM3.
Camilla TO Benfield, Farrell MacKenzie, Markus Ritzefeld, Michela Mazzon, Stuart Weston, Edward W Tate, Boon Han Teo, Sarah E Smith, Paul Kellam, Edward C Holmes, Mark Marsh   +10 more
doaj   +1 more source

SUMO-interacting motifs of human TRIM5α are important for antiviral activity. [PDF]

PLoS Pathogens, 2011
Human TRIM5α potently restricts particular strains of murine leukemia viruses (the so-called N-tropic strains) but not others (the B- or NB-tropic strains) during early stages of infection.
Gloria Arriagada, Lucia N Muntean, Stephen P Goff   +2 more
doaj   +1 more source

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae [PDF]

, 1978
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the ...
Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R. J.   +3 more
core   +1 more source

Internal Restriction Sites: Quality Assurance Aids in Genotyping [PDF]

Journal of Veterinary Diagnostic Investigation, 2006
Improvements to restriction fragment length polymorphism (RFLP)-based genotyping assays currently used for detection of mutations responsible for bovine ferrochelatase and myophosphorylase deficiencies, and equine hyperkalemic periodic paralysis (HYPP) are described.
Brendon A, O'Rourke, Julie A, Dennis, Peter J, Healy   +2 more
openaire   +2 more sources

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil [PDF]

, 2001
Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum
Adolphe Zeze, Amarger N., Bromfield E.S.P., Chanway C.P., Demezas D.H., Eardly B.D., Harrison S.P., Harrison S.P., Haukka K., Hirsch P.R., J. Peter W. Young, Jones D.G., Kaijalainen S., Kirshtein J.D., Laguerre G., Laguerre G., Laguerre G., Lesley A. Mutch, Leung K., Lieven-Antoniou C.A., Liu W.T., Louvrier P., McDonald I.R., Page R.D.M., Saitou N., Sambrook J., Segovia L., Smalla K., Strain S.R., Sullivan J.T., Turner S.L., Young J.P.W., Young J.P.W., Young J.P.W., Zhou J.Z.   +34 more
core   +1 more source

PHYLOGENIES FROM RESTRICTION SITES: A MAXIMUM‐LIKELIHOOD APPROACH [PDF]

Evolution, 1992
Restriction sites data can be analyzed by maximum likelihood to obtain estimates of phylogenies. The likelihood methods of Smouse and Li, who were able to compute likelihoods for up to four species under a simplified model of base change, can be extended numerically to deal with any number of species. The computational methods for doing so are outlined.
openaire   +2 more sources