Results 111 to 120 of about 6,061,790 (368)
Collapse transition in polymer models with multiple monomers per site and multiple bonds per edge [PDF]
, 2017 We present results from extensive Monte Carlo simulations of polymer models where each lattice site can be visited by up to $K$ monomers and no restriction is imposed on the number of bonds on each lattice edge.
Oliveira, Tiago J. +1 more
core +2 more sources
Molecular Plant-Microbe Interactions, 2000 We have developed a restriction enzyme-mediated insertional mutagenesis (REMI) system for the maize pathogen Colletotrichum graminicola. In this report, we demonstrate the utility of a REMI-based mutagenesis approach to identify novel pathogenicity genes.
M. R. Thon +2 more
doaj +1 more source
Genome Biology, 2010 High-throughput genotype data can be used to identify genes important for local adaptation in wild populations, phenotypes in lab stocks, or disease-related traits in human medicine.
Melissa H. Pespeni +3 more
semanticscholar +1 more source
Evolutionary interplay between viruses and R‐loops
FEBS Letters, EarlyView.Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi +4 more
wiley +1 more source
Method for Cloning Restriction Fragments Containing the Termini of BAC Inserts
BioTechniques, 2000 We have developed a method to isolate the termini of BAC clones. The method is based on the two unique NotI sites located approximately 300 bp on either side of the EcoRI cloning site of the BAC vector pECSBAC4. Our strategy includes the following steps:
Tammy R. Plyler, C. Eduardo Vallejos
doaj +1 more source
BMC Bioinformatics, 2013 Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert.
Abhijit Karnik +2 more
semanticscholar +1 more source
Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation
FEBS Letters, EarlyView.The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt +8 more
wiley +1 more source
Lignin‐based barrier restricts pathogens to the infection site and confers resistance in plants
EMBO Journal, 2019 Pathogenic bacteria invade plant tissues and proliferate in the extracellular space. Plants have evolved the immune system to recognize and limit the growth of pathogens. Despite substantial progress in the study of plant immunity, the mechanism by which
Myoung-Hoon Lee +9 more
semanticscholar +1 more source
Purification tags markedly affect self‐aggregation of CPEB3
FEBS Letters, EarlyView.Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito +6 more
wiley +1 more source
Spatial search in a honeycomb network
, 2010 The spatial search problem consists in minimizing the number of steps required to find a given site in a network, under the restriction that only oracle queries or translations to neighboring sites are allowed.
F. L. MARQUEZINO +5 more
core +1 more source