Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM
FEBS Letters, EarlyView.Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley +1 more source
Mapping the evolution of mitochondrial complex I through structural variation
FEBS Letters, EarlyView.Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin +2 more
wiley +1 more source
Journal of the Indonesian Tropical Animal Agriculture, 2014 The objective of this research was to identify growth hormone (GH) genes genotype in selectedAceh cattle at Indrapuri’s Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattleconsisting of 21 male and 20 female cattle were used in this study.
W.P.B. Putra, T. Hartatik, S. Sumadi
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Use of a Sequence‐Specific DNA‐Binding Ligand to Probe the Environments of EcoRI Restriction Endonuclease Cleavage Sites [PDF]
, 1976 Jürgen Kania, Thomas G. Fanning
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Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target
FEBS Letters, EarlyView.This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz +5 more
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Restriction site associated DNA sequencing for tumour mutation burden estimation and mutation signature analysis. [PDF]
Cancer Med, 2023 McGuinness CF, Black MA, Dunbier AK.
europepmc +1 more source
FEBS Letters, EarlyView.Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur +5 more
wiley +1 more source
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly. [PDF]
Cell Rep Methods, 2023 Sorida M, Bonasio R.
europepmc +1 more source
Reciprocal control of viral infection and phosphoinositide dynamics
FEBS Letters, EarlyView.Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley +1 more source
Golden EGG, a simplified Golden Gate cloning system to assemble multiple fragments
Scientific ReportsThe Golden Gate method is an efficient tool for seamless assembly of multiple DNA fragments, which uses Type IIS restriction endonucleases, cleaving the DNA outside of their recognition site to release DNA parts from PCR fragments or entry clones, thus ...
János Barnabás Biró +6 more
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