Golden EGG, a simplified Golden Gate cloning system to assemble multiple fragments
Scientific ReportsThe Golden Gate method is an efficient tool for seamless assembly of multiple DNA fragments, which uses Type IIS restriction endonucleases, cleaving the DNA outside of their recognition site to release DNA parts from PCR fragments or entry clones, thus ...
János Barnabás Biró +6 more
doaj +1 more source
Mechanisms of parasite‐mediated disruption of brain vessels
FEBS Letters, EarlyView.Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira +3 more
wiley +1 more source
Restriction site associated DNA sequencing for tumour mutation burden estimation and mutation signature analysis. [PDF]
Cancer Med, 2023 McGuinness CF, Black MA, Dunbier AK.
europepmc +1 more source
Cleavage sites of restriction endonucleases EcoRI, BamHI and HindIII on chicken embryo rDNA [PDF]
, 1979 Walter Knöchel +2 more
openalex +1 more source
Time after time – circadian clocks through the lens of oscillator theory
FEBS Letters, EarlyView.Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo +2 more
wiley +1 more source
An efficient cloning method to expand vector and restriction site compatibility of Golden Gate Assembly. [PDF]
Cell Rep Methods, 2023 Sorida M, Bonasio R.
europepmc +1 more source
The newfound relationship between extrachromosomal DNAs and excised signal circles
FEBS Letters, EarlyView.Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley +1 more source
Analysis of the Genetic Diversity in Tea Plant Germplasm in Fujian Province Based on Restriction Site-Associated DNA Sequencing. [PDF]
Plants (Basel), 2023 Jiang L +8 more
europepmc +1 more source
Many Type IIs Restriction Endonucleases Interact with Two Recognition Sites before Cleaving DNA [PDF]
, 2002 Abigail J. Bath +3 more
openalex +1 more source