Construction of a highly saturated Genetic Map for Vitis by Next-generation Restriction Site-associated DNA Sequencing [PDF]
High-saturate molecular linkage maps are an important tool in studies on plant molecular biology and assisted breeding. Development of a large set of single nucleotide polymorphisms (SNPs) via next-generation sequencing (NGS)-based methods, restriction ...
Junchi Zhu+6 more
semanticscholar +2 more sources
Re-evaluating the Systematics of Dendrolycopodium Using Restriction-Site Associated DNA-Sequencing [PDF]
The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or inconsistent characters.
Alaina R. Petlewski+8 more
doaj +2 more sources
Unidirectional diploid–tetraploid introgression among British birch trees with shifting ranges shown by restriction site‐associated markers [PDF]
Hybridization may lead to introgression of genes among species. Introgression may be bidirectional or unidirectional, depending on factors such as the demography of the hybridizing species, or the nature of reproductive barriers between them.
Jasmin Zohren+6 more
semanticscholar +2 more sources
Breaking RAD: an evaluation of the utility of restriction site‐associated DNA sequencing for genome scans of adaptation [PDF]
D. Lowry+6 more
semanticscholar +2 more sources
indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events. [PDF]
Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations
Charles Hodgens+2 more
doaj +1 more source
A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus. [PDF]
PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion.
Maria W Smith+5 more
doaj +1 more source
Site-specific restriction endonucleases in cyanobacteria [PDF]
Planktic cyanobacteria were screened for endodeoxyribonucleases. Principal component analysis (PCA) was employed to demonstrate a potential relationship between certain enzymes and a group of cyanobacteria. The data were obtained from a data bank and this study.Enzymes were partially purified using column chromatography.
A.-M. Torsti+4 more
openaire +3 more sources
Combinatoric Properties of Restriction Sites [PDF]
Endonucleases from the restriction–modification complex are known to recognise specific sites with tremendous precision. [...]
Elizaveta Taranenko, Mikhail Sadovsky
openaire +2 more sources
Special features of RAD Sequencing data:implications for genotyping [PDF]
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Blaxter, Mark L+5 more
core +1 more source
We have investigated the temporal distribution of dengue (DEN) virus serotypes in the department (state) of Santander, Colombia, in relation to dengue incidence, infection pattern, and severity of disease.
Raquel Elvira Ocazionez+3 more
doaj +1 more source