Re-evaluating the Systematics of Dendrolycopodium Using Restriction-Site Associated DNA-Sequencing [PDF]
Frontiers in Plant Science, 2022 The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or inconsistent characters.
Alaina R. Petlewski +8 more
doaj +2 more sources
Restriction Site-Free Insertion of PCR Products Directionally into Vectors [PDF]
BioTechniques, 2000 A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions.
G.J. Chen +4 more
doaj +2 more sources
Molecular Mapping of Restriction-Site Associated DNA Markers In Allotetraploid Upland Cotton. [PDF]
PLoS ONE, 2015 Upland cotton (Gossypium hirsutum L., 2n = 52, AADD) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (SNP) markers is difficult. The recent emergence of genome complexity reduction technologies based on the next-generation
Yangkun Wang +8 more
doaj +2 more sources
Growth Hormone Gene Genotyping by Msp I Restriction Enzyme and Pcr-rflp Methods in Aceh Cattle Breed at Indrapuri District of Aceh Province [PDF]
Journal of the Indonesian Tropical Animal Agriculture, 2013 The objective of this research was to identify growth hormone (GH) genes genotype in selectedAceh cattle at Indrapuri\u27s Breeding and Forage Centre (IBFC) of Aceh Cattle.
Hartatik, T. (T) +2 more
core +3 more sources
Frontiers in Plant Science, 2021 Genome walking (GW), a strategy for capturing previously unsequenced DNA fragments that are in proximity to a known sequence tag, is currently predominantly based on PCR.
Ruslan Kalendar +4 more
doaj +1 more source
Complete genome assembly and methylome dissection of Methanococcus aeolicus PL15/Hp
Frontiers in Microbiology, 2023 Although restriction-modification systems are found in both Eubacterial and Archaeal kingdoms, comparatively less is known about patterns of DNA methylation and genome defense systems in archaea.
Alexey Fomenkov +4 more
doaj +1 more source
The Genetic Characterization of DGAT1 Gene in Donkey Populations Reared in Thrace Region of Turkey
Journal of Agricultural Sciences, 2020 AcylCoA: diacylglycerol acyltransferase (DGAT1) gene has a considerable effect on milk content and yield in cattle with a substitution of lysine by alanine in the exon 8 of the gene.
Fulya Özdil
doaj +1 more source
A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus. [PDF]
PLoS ONE, 2014 PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion.
Maria W Smith +5 more
doaj +1 more source
indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events. [PDF]
PLoS ONE, 2017 Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations
Charles Hodgens +2 more
doaj +1 more source
Memorias do Instituto Oswaldo Cruz, 2006 We have investigated the temporal distribution of dengue (DEN) virus serotypes in the department (state) of Santander, Colombia, in relation to dengue incidence, infection pattern, and severity of disease.
Raquel Elvira Ocazionez +3 more
doaj +1 more source