BBF RFC #111: Inducible expression vector with His tag fusion for purification of RFC25 parts [PDF]
, 2015 This Request For Comments (RFC) describes a new expression vector based on the pET expression system and designed for BioBrick RFC25 parts. This design includes all the features of pET expression vectors with a new origin of replication, resistance ...
Husser, Mathieu +2 more
core +15 more sources
The Genetic Characterization of DGAT1 Gene in Donkey Populations Reared in Thrace Region of Turkey
Journal of Agricultural Sciences, 2020 AcylCoA: diacylglycerol acyltransferase (DGAT1) gene has a considerable effect on milk content and yield in cattle with a substitution of lysine by alanine in the exon 8 of the gene.
Fulya Özdil
doaj +1 more source
A new restriction endonuclease-based method for highly-specific detection of DNA targets from methicillin-resistant Staphylococcus aureus. [PDF]
PLoS ONE, 2014 PCR multiplexing has proven to be challenging, and thus has provided limited means for pathogen genotyping. We developed a new approach for analysis of PCR amplicons based on restriction endonuclease digestion.
Maria W Smith +5 more
doaj +1 more source
indCAPS: A tool for designing screening primers for CRISPR/Cas9 mutagenesis events. [PDF]
PLoS ONE, 2017 Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations
Charles Hodgens +2 more
doaj +1 more source
Memorias do Instituto Oswaldo Cruz, 2006 We have investigated the temporal distribution of dengue (DEN) virus serotypes in the department (state) of Santander, Colombia, in relation to dengue incidence, infection pattern, and severity of disease.
Raquel Elvira Ocazionez +3 more
doaj +1 more source
Selective Protection of Restriction Endonuclease Sites [PDF]
BioTechniques, 2003 A difficulty that is encountered when attempting to insert a PCR-amplified product or DNA fragment of interest into a particular vector is the presence within the insert of one or more internal restriction endonuclease (RE) sites identical to those selected for the flanks of the insert.
Kagnew H. Gebreyesus, Roland A. Owens
openaire +3 more sources
Conformational adaptation of Asian macaque TRIMCyp directs lineage specific antiviral activity [PDF]
, 2010 TRIMCyps are anti-retroviral proteins that have arisen independently in New World and Old World primates. All TRIMCyps comprise a CypA domain fused to the tripartite domains of TRIM5α but they have distinct lentiviral specificities, conferring HIV-1 ...
A Katzourakis +62 more
core +5 more sources
Baboon endogenous virus genome: Molecular cloning and structural characterization of nondefective viral genomes from DNA of a baboon cell strain [PDF]
, 1981 Several heterogeneities in the baboon endogenous virus (BaEV) genomes that are present in the DNA of normal baboon tissues and the baboon cell strain BEF-3 have been described previously.
Cohen, Maurice +8 more
core +1 more source
Application of Oligonucleotide Activation to Restriction Endonuclease NarI
BioTechniques, 1997 Restriction endonuclease NarI cleaves DNA using a two-site mechanism, placing it in the Type IIe class of restriction endonucleases. Although these enzymes have very useful recognition sequences, the two-site mechanism limits the practical application ...
Joseph H. Senesac, Jennifer K. Romanin
doaj +1 more source
SOMA: a single oligonucleotide mutagenesis and cloning approach. [PDF]
PLoS ONE, 2013 Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of ...
Thorsten Pfirrmann +4 more
doaj +1 more source