Results 251 to 260 of about 1,167,532 (299)
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Errors between sites in restriction site mapping
Bioinformatics, 1988Restriction site mapping programs construct maps by generating permutations of fragments and checking for consistency. Unfortunately many consistent maps often are obtained within the experimental error bounds, even though there is only one actual map.
T I, Dix, D H, Kieronska
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Promoter vectors with restriction-site banks
Gene, 1988New vectors harboring the promoter for the chloramphenicol acetyl transferase gene (cat promoter) have been constructed. These vectors are all derived from pJRD184 [Heusterspreute et al., Gene 39 (1985) 299-304], which contains a restriction-site bank.
Bleicher, F. +5 more
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Evolutionary Dynamics of Restriction Site Avoidance
Physical Review Letters, 2012Molecular noise in bacterial restriction-modification systems can cause rare events of host DNA cleavage at restriction sites. Such noise-induced selective pressure may result in evolved sequences exhibiting restriction site avoidance. We identify a two-state regime of evolutionary dynamics, in which populations either develop avoidance or go extinct ...
Long, Qian, Edo, Kussell
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2022
This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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Differential reactivities at restriction enzyme sites
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981A method has been developed to measure the rates of digestion by restriction enzymes at individual sites. This involves a simple arithmetical treatment of the integrated areas from a densitometer scan of an ethidium bromide stained gel. We have used this method to study the digestion by HpaI, HincII and SalI of pBR322 and phi X174 DNA, and the effect ...
A D, Malcolm, J R, Moffatt
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The cleavage site of restriction endonuclease MnlI
Gene, 1991Abstract The cleavage site generated by restriction endonuclease MnlI has the structure: 5′-CCTC(N) 7 −3′ 3′-GGAG(N) 6 −5″ with one-nucleotide 3′ overhang.
P, Brinkley, D S, Bautista, F L, Graham
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Practical consequences of restriction site symmetry
Gene, 1984Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often ...
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Restriction site mapping is in separation theory
Bioinformatics, 1988A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
L, Allison, C N, Yee
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DNA fingerprinting by infrequent-restriction-site amplification
Journal of Clinical Microbiology, 1996Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce ...
G H, Mazurek +4 more
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Restriction endonucleases functionally interacting with two DNA sites
Gene, 1995Simultaneous interaction with two recognition sites was found to be a precondition for DNA cleavage by certain type-II and type-III restriction endonucleases. Nevertheless, the molecular mechanisms of the protein-DNA interaction are different between members of both classes of enzymes.
D H, Krüger +5 more
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