Site-specific restriction endonucleases in cyanobacteria [PDF]
Planktic cyanobacteria were screened for endodeoxyribonucleases. Principal component analysis (PCA) was employed to demonstrate a potential relationship between certain enzymes and a group of cyanobacteria. The data were obtained from a data bank and this study.Enzymes were partially purified using column chromatography.
C, Lyra +4 more
openaire +2 more sources
Sequence organization of feline leukemis virus DNA in infected cells [PDF]
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
core +1 more source
Special features of RAD Sequencing data:implications for genotyping [PDF]
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Blaxter, Mark L +5 more
core +1 more source
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +1 more source
Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems
ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of the type I restriction-modification enzymes (RMI). In this study, we evaluated the ability of ArdB, ArdA, and Ocr to inhibit different subtypes of Escherichia coli RMI systems (IA, IB, and
Anna A. Kudryavtseva +12 more
doaj +1 more source
Differentiation between Aspergillus flavus and Aspergillus parasiticus from Pure Culture and Aflatoxin-Contaminated Grapes Using PCR-RFLP Analysis of aflR-aflJ Intergenic Spacer [PDF]
Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
Atoui, Ali +5 more
core +2 more sources
Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov +4 more
doaj +1 more source
Finding restriction sites [PDF]
Webcutter provides a free and easy-to-use interface for the analysis of restriction sites in any sequence.
openaire +1 more source
Chimeric Gene Construction without Reference to Restriction Sites [PDF]
Toshiro Shigaki, Kendal D. Hirschi
openaire +4 more sources
Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP [PDF]
The objective of this work was to develop a rapid screening method to identify the three single nucleotide polymorphisms (SNPs) in the TAS2R38 gene, with the aim of providing a significant contribution to studies designed to assess sensitivity to the ...
Atzori, E, Orrù, R, Padiglia, A
core +2 more sources

