Baboon endogenous virus genome: Molecular cloning and structural characterization of nondefective viral genomes from DNA of a baboon cell strain [PDF]
, 1981 Several heterogeneities in the baboon endogenous virus (BaEV) genomes that are present in the DNA of normal baboon tissues and the baboon cell strain BEF-3 have been described previously.
Cohen, Maurice +8 more
core +1 more source
Application of Oligonucleotide Activation to Restriction Endonuclease NarI
BioTechniques, 1997 Restriction endonuclease NarI cleaves DNA using a two-site mechanism, placing it in the Type IIe class of restriction endonucleases. Although these enzymes have very useful recognition sequences, the two-site mechanism limits the practical application ...
Joseph H. Senesac, Jennifer K. Romanin
doaj +1 more source
SOMA: a single oligonucleotide mutagenesis and cloning approach. [PDF]
PLoS ONE, 2013 Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of ...
Thorsten Pfirrmann +4 more
doaj +1 more source
Conformational adaptation of Asian macaque TRIMCyp directs lineage specific antiviral activity [PDF]
, 2010 TRIMCyps are anti-retroviral proteins that have arisen independently in New World and Old World primates. All TRIMCyps comprise a CypA domain fused to the tripartite domains of TRIM5α but they have distinct lentiviral specificities, conferring HIV-1 ...
A Katzourakis +62 more
core +5 more sources
Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems
Frontiers in Microbiology, 2023 ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of the type I restriction-modification enzymes (RMI). In this study, we evaluated the ability of ArdB, ArdA, and Ocr to inhibit different subtypes of Escherichia coli RMI systems (IA, IB, and
Anna A. Kudryavtseva +12 more
doaj +1 more source
Restriction site patterns in the ribosomal DNA of Camelidae [PDF]
Genetica, 1994 The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI,BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences.
Semorile, Liliana Carmen +2 more
openaire +3 more sources
Sequence organization of feline leukemis virus DNA in infected cells [PDF]
, 1980 A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
core +1 more source
Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex [PDF]
, 2020 Background: Chromosomal inversion polymorphisms play a role in adaptation to heterogeneous environments. Inversion polymorphisms are implicated in the very high ecological flexibility of the three main malaria vector species of the Afrotropical Anopheles
Besansky, N. J. +10 more
core +1 more source
BMC Genomics, 2018 Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov +4 more
doaj +1 more source
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
PLoS ONE, 2014 The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +1 more source