SOMA: a single oligonucleotide mutagenesis and cloning approach. [PDF]
PLoS ONE, 2013 Modern biology research requires simple techniques for efficient and restriction site-independent modification of genetic material. Classical cloning and mutagenesis strategies are limited by their dependency on restriction sites and the use of ...
Thorsten Pfirrmann +4 more
doaj +1 more source
Sequence organization of feline leukemis virus DNA in infected cells [PDF]
, 1980 A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV.
Casey, James W. +3 more
core +1 more source
Differentiation between Aspergillus flavus and Aspergillus parasiticus from Pure Culture and Aflatoxin-Contaminated Grapes Using PCR-RFLP Analysis of aflR-aflJ Intergenic Spacer [PDF]
, 2011 Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
Atoui, Ali +5 more
core +1 more source
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
PLoS ONE, 2014 The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +1 more source
Broadness and specificity: ArdB, ArdA, and Ocr against various restriction-modification systems
Frontiers in Microbiology, 2023 ArdB, ArdA, and Ocr proteins inhibit the endonuclease activity of the type I restriction-modification enzymes (RMI). In this study, we evaluated the ability of ArdB, ArdA, and Ocr to inhibit different subtypes of Escherichia coli RMI systems (IA, IB, and
Anna A. Kudryavtseva +12 more
doaj +1 more source
Restriction site patterns in the ribosomal DNA of Camelidae [PDF]
Genetica, 1994 The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI,BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences.
Semorile, Liliana Carmen +2 more
openaire +3 more sources
Special features of RAD Sequencing data:implications for genotyping [PDF]
, 2012 Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Blaxter, Mark L +5 more
core +1 more source
BMC Genomics, 2018 Background Restriction-modification (R-M) systems protect bacteria and archaea from attacks by bacteriophages and archaeal viruses. An R-M system specifically recognizes short sites in foreign DNA and cleaves it, while such sites in the host DNA are ...
I. S. Rusinov +4 more
doaj +1 more source
Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP [PDF]
, 2015 The objective of this work was to develop a rapid screening method to identify the three single nucleotide polymorphisms (SNPs) in the TAS2R38 gene, with the aim of providing a significant contribution to studies designed to assess sensitivity to the ...
Atzori, E, Orrù, R, Padiglia, A
core +2 more sources
Chimeric Gene Construction without Reference to Restriction Sites [PDF]
BioTechniques, 2002 Toshiro Shigaki +2 more
openaire +5 more sources