Results 291 to 300 of about 1,152,156 (344)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

BlihIA-A Novel Type I Restriction-Modification System from <i>Bacillus licheniformis</i> Is Sensitive to In Vitro Inhibition by ArdB Antirestriction Protein. [PDF]

Int J Mol Sci
Kudryavtseva A, Berezov R, Utkina A, Kotovskaya O, Skutel M, Trofimova A, Isaev A, Manukhov I.   +7 more
europepmc   +1 more source

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Errors between sites in restriction site mapping

Bioinformatics, 1988
Restriction site mapping programs construct maps by generating permutations of fragments and checking for consistency. Unfortunately many consistent maps often are obtained within the experimental error bounds, even though there is only one actual map.
Dorota H. Kieronska, Trevor I. Dix
openaire   +3 more sources

Mapping of restriction sites in the attachment site region of bacteriophage lambda [PDF]

Molecular and General Genetics MGG, 1977
A find structure map of the EcoRI fragment containing the lambda attachment-site region has been constructed. 38 different restriction endonucleases have been employed and 170 sites located in this fragment. In addition, sites in adjacent regions have been determined for several enzymes.
David Zipser, Dietmar Kamp, Regine Kahmann, Richard J. Roberts   +3 more
openaire   +2 more sources

Promoter vectors with restriction-site banks

Gene, 1988
New vectors harboring the promoter for the chloramphenicol acetyl transferase gene (cat promoter) have been constructed. These vectors are all derived from pJRD184 [Heusterspreute et al., Gene 39 (1985) 299-304], which contains a restriction-site bank.
Bleicher, F., Deville, F., Cortay, Jc, Negre, D., Cozzone, Aj, Cenatiempo, Y.   +5 more
openaire   +5 more sources

Evolutionary Dynamics of Restriction Site Avoidance

Physical Review Letters, 2012
Molecular noise in bacterial restriction-modification systems can cause rare events of host DNA cleavage at restriction sites. Such noise-induced selective pressure may result in evolved sequences exhibiting restriction site avoidance. We identify a two-state regime of evolutionary dynamics, in which populations either develop avoidance or go extinct ...
Long Qian, Edo Kussell
openaire   +3 more sources

The cleavage site of restriction endonuclease MnlI

Gene, 1991
Abstract The cleavage site generated by restriction endonuclease MnlI has the structure: 5′-CCTC(N) 7 −3′ 3′-GGAG(N) 6 −5″ with one-nucleotide 3′ overhang.
Frank L. Graham, Diosdado S. Bautista, Pamela Brinkley   +2 more
openaire   +3 more sources