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2022
This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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Restriction site mapping is in separation theory
Bioinformatics, 1988A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
Chut N. Yee, Lloyd Allison
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Differential reactivities at restriction enzyme sites
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981A method has been developed to measure the rates of digestion by restriction enzymes at individual sites. This involves a simple arithmetical treatment of the integrated areas from a densitometer scan of an ethidium bromide stained gel. We have used this method to study the digestion by HpaI, HincII and SalI of pBR322 and phi X174 DNA, and the effect ...
John R. Moffatt, Alan D. B. Malcolm
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A partial digest approach to restriction site mapping
Bulletin of Mathematical Biology, 1994We present a new practical algorithm to resolve the experimental data of restriction site analysis, which is a common technique for mapping DNA. Specifically, we assert that multiple digests with a single restriction enzyme can provide sufficient information to identify the positions of the restriction sites with high probability.
Gopalakrishnan Sundaram, Steven Skiena
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Practical consequences of restriction site symmetry
Gene, 1984Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often ...
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[33] Phylogenetic analysis of restriction site data
1993Publisher Summary This chapter reviews different methods for the phylogenetic analysis of restriction site variation. It illustrates how these methods can be used by applying them to restriction site variation among genera within a subtribe of the sunflower family. Restriction fragment patterns can be characterized either as a simple list of fragment
Robert K. Jansen, Kent E. Holsinger
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Restriction sites as identification tags for lymphocyte cDNAs
ELECTROPHORESIS, 1997AbstractA cDNA library was prepared from BW 5147 murine lymphoma cells in λ ecc III phage and randomly partitioned into 291 sectors, each with 800–1000 recombinant phage plaques. One sector was chosen for further characterization in terms of sensitivity to restriction endonuclease cutting. Aliquots of DNA preparations from this sector were treated with
Johann Rudolf Frey +2 more
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Constraint checking for restriction site mapping
Proceedings of the Twenty-Fourth Annual Hawaii International Conference on System Sciences, 2002Introduces an algorithm for the restriction site mapping problem, based on Pratt's separation theory. The algorithm accepts data from single and double digests of a DNA molecule, in which every fragment is given a range of possible lengths depending on experimental errors. As fragments are being assembled into potential maps of the original molecule, a
C.N. Yee +3 more
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Effect of Restricting Access to a Suicide Jumping Site
Australian & New Zealand Journal of Psychiatry, 2009Objective: The road to a headland that had become a suicide jumping hotspot was temporarily closed because of construction work. This created an opportunity to assess whether loss of vehicular access would lead to a reduction in suicides and emergency police callouts for threatened suicide at the site.
Peter Herbison, Keren Skegg
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Regular arrangement of restriction sites in drosophila DNA
Cell, 1975When DNA of Drosophila melanogaster is digested to completion with Hemophilus aegyptius restriction endonuclease, the majority of the products are DNA segments whose lengths fits a random distribution with an average of 350 base pairs. However, some 10% of the DNA is recovered as various segments of discrete lengths, ranging from 30,000 to 365 base ...
Charles A. Thomas +2 more
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