Results 301 to 310 of about 1,179,472 (345)
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The cleavage site of restriction endonuclease MnlI
Gene, 1991Abstract The cleavage site generated by restriction endonuclease MnlI has the structure: 5′-CCTC(N) 7 −3′ 3′-GGAG(N) 6 −5″ with one-nucleotide 3′ overhang.
P, Brinkley, D S, Bautista, F L, Graham
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Practical consequences of restriction site symmetry
Gene, 1984Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often ...
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Restriction site mapping is in separation theory
Bioinformatics, 1988A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
L, Allison, C N, Yee
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DNA fingerprinting by infrequent-restriction-site amplification
Journal of Clinical Microbiology, 1996Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce ...
G H, Mazurek +4 more
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Restriction endonucleases functionally interacting with two DNA sites
Gene, 1995Simultaneous interaction with two recognition sites was found to be a precondition for DNA cleavage by certain type-II and type-III restriction endonucleases. Nevertheless, the molecular mechanisms of the protein-DNA interaction are different between members of both classes of enzymes.
D H, Krüger +5 more
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Constraint checking for restriction site mapping
Proceedings of the Twenty-Fourth Annual Hawaii International Conference on System Sciences, 2002Introduces an algorithm for the restriction site mapping problem, based on Pratt's separation theory. The algorithm accepts data from single and double digests of a DNA molecule, in which every fragment is given a range of possible lengths depending on experimental errors. As fragments are being assembled into potential maps of the original molecule, a
S.T.S. Ho, L. Allison, C.N. Yee, T. Dix
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Restriction sites as identification tags for lymphocyte cDNAs
ELECTROPHORESIS, 1997AbstractA cDNA library was prepared from BW 5147 murine lymphoma cells in λ ecc III phage and randomly partitioned into 291 sectors, each with 800–1000 recombinant phage plaques. One sector was chosen for further characterization in terms of sensitivity to restriction endonuclease cutting. Aliquots of DNA preparations from this sector were treated with
J R, Frey, B, Fol, I, Lefkovits
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Restriction endonuclease sites and aromatic metabolic plasmid structure
Biochemical and Biophysical Research Communications, 1978Plasmid DNA isolated from Pseudomonas putida strains which metabolize naphthalene (Nah+), salicylate (Sal+), or toluate (Tol+) contain multiple restriction endonuclease sites. Eco R1, Bam H1, and Hind III produce 10, 11 and 12 fragments respectively from plasmids NAH (∼48 Md)§ and SAL (∼49 Md).
R, Farrell +4 more
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Brain and other central nervous system tumor statistics, 2021
Ca-A Cancer Journal for Clinicians, 2021Kimberly D Miller +2 more
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