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Promoter vectors with restriction-site banks
Gene, 1988New vectors harboring the promoter for the chloramphenicol acetyl transferase gene (cat promoter) have been constructed. These vectors are all derived from pJRD184 [Heusterspreute et al., Gene 39 (1985) 299-304], which contains a restriction-site bank.
Bleicher, F. +5 more
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The cleavage site of restriction endonuclease MnlI
Gene, 1991Abstract The cleavage site generated by restriction endonuclease MnlI has the structure: 5′-CCTC(N) 7 −3′ 3′-GGAG(N) 6 −5″ with one-nucleotide 3′ overhang.
Frank L. Graham +2 more
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2022
This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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This thesis was scanned from the print manuscript for digital preservation and is copyright the author. Researchers can access this thesis by asking their local university, institution or public library to make a request on their behalf. Monash staff and postgraduate students can use the link in the References field.
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Restriction site mapping is in separation theory
Bioinformatics, 1988A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
Chut N. Yee, Lloyd Allison
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SNP discovery and genotyping using restriction-site-associated DNA sequencing in chickens.
Animal Genetics, 2015Single nucleotide polymorphisms (SNPs) are essential to the understanding of population genetic variation and diversity. Here, we performed restriction-site-associated DNA sequencing (RAD-seq) on 72 individuals from 13 Chinese indigenous and three ...
Zhengxiao Zhai +8 more
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Differential reactivities at restriction enzyme sites
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981A method has been developed to measure the rates of digestion by restriction enzymes at individual sites. This involves a simple arithmetical treatment of the integrated areas from a densitometer scan of an ethidium bromide stained gel. We have used this method to study the digestion by HpaI, HincII and SalI of pBR322 and phi X174 DNA, and the effect ...
John R. Moffatt, Alan D. B. Malcolm
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A partial digest approach to restriction site mapping
Bulletin of Mathematical Biology, 1994We present a new practical algorithm to resolve the experimental data of restriction site analysis, which is a common technique for mapping DNA. Specifically, we assert that multiple digests with a single restriction enzyme can provide sufficient information to identify the positions of the restriction sites with high probability.
Gopalakrishnan Sundaram, Steven Skiena
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Practical consequences of restriction site symmetry
Gene, 1984Because of the palindromic character of most 6-bp restriction sites, filling-in and ligation of the protruding ends create symmetric sequences which include new 6-bp restriction sites. The old site is, in most cases, lost. After cleavage at the new palindromic site and removal of the protruding ends, a new center of symmetry is created which is often ...
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[33] Phylogenetic analysis of restriction site data
1993Publisher Summary This chapter reviews different methods for the phylogenetic analysis of restriction site variation. It illustrates how these methods can be used by applying them to restriction site variation among genera within a subtribe of the sunflower family. Restriction fragment patterns can be characterized either as a simple list of fragment
Robert K. Jansen, Kent E. Holsinger
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Constraint checking for restriction site mapping
Proceedings of the Twenty-Fourth Annual Hawaii International Conference on System Sciences, 2002Introduces an algorithm for the restriction site mapping problem, based on Pratt's separation theory. The algorithm accepts data from single and double digests of a DNA molecule, in which every fragment is given a range of possible lengths depending on experimental errors. As fragments are being assembled into potential maps of the original molecule, a
C.N. Yee +3 more
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