Results 31 to 40 of about 1,093,792 (283)
Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
Scientific Reports, 2021 Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting ...
Federico Colombo +3 more
doaj +1 more source
A one pot, one step, precision cloning method with high throughput capability.
PLoS ONE, 2008 Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed ...
Carola Engler +2 more
doaj +1 more source
Rapid Generation of Nested Deletions by Differential Restriction Digestion
BioTechniques, 2002 A method was devised for generating nested deletions in DNA that exploits the difference in frequency of restriction sites recognized by compatible restriction endonucleases.
Jonathan J. Dennis, Gerben J. Zylstra
doaj +1 more source
, 2015 We investigate the semi-ringed topos obtained by extension of scalars from the arithmetic site of our previous work, by replacing the smallest Boolean semifield by the tropical semifield of real numbers with the max-plus operations.
Connes, Alain, Consani, Caterina
core +3 more sources
Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat
Tropical Animal Science Journal, 2023 Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik +6 more
doaj +1 more source
N-Site approximations and CAM analysis for a stochastic sandpile
, 2002 I develop n-site cluster approximations for a stochastic sandpile in one dimension. A height restriction is imposed to limit the number of states: each site can harbor at most two particles (height z_i \leq 2).
A. Chessa +34 more
core +1 more source
Network topology drives population temporal variability in experimental habitat networks
Population Ecology, EarlyView.Habitat patches connected by dispersal pathways form habitat networks. We explored how network topology affects population outcomes in laboratory experiments using a model species (Daphnia carinata). Central habitat nodes in complex lattice networks exhibited lower temporal variability in population sizes, suggesting they support more stable ...
Yiwen Xu +3 more
wiley +1 more source
Frontiers in Marine Science, 2023 Montipora digitata is a species belonging to the Acroporidae. In the Indo-Pacific region, M. digitata is widely distributed and is the dominant species of scleractinian coral in the South China Sea, however, there are currently no molecular markers ...
Shuwen Jia +7 more
doaj +1 more source
Baboon endogenous virus genome. I. Restriction enzyme map of the unintegrated DNA genome of a primate retrovirus [PDF]
, 1980 A detailed restriction map was deduced for the genome of an endogenous retrovirus of a higher primate, that of baboon. The cleavage sites for 12 restriction enzymes were mapped. The unintegrated linear viral DNA intermediate that is produced by infection
Cohen, Maurice +6 more
core
Plasma Membrane-Associated Restriction Factors and Their Counteraction by HIV-1 Accessory Proteins. [PDF]
, 2019 The plasma membrane is a site of conflict between host defenses and many viruses. One aspect of this conflict is the host's attempt to eliminate infected cells using innate and adaptive cell-mediated immune mechanisms that recognize features of the ...
Guatelli, John +5 more
core +1 more source