Results 31 to 40 of about 1,152,156 (344)
Universal Restriction Site-Free Cloning Method Using Chimeric Primers
BioTechniques, 2002 A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector.
Guo Jun Chen +2 more
doaj +1 more source
Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns [PDF]
, 1933 Hepatitis B virus (HBV) is classified into genotypes A–F, which is important for clinical and etiological investigations. To establish a simple genotyping method, 68 full-genomic sequences and 106 S gene sequences were analyzed by the molecular ...
Mizokami, Masashi +6 more
core +2 more sources
Using indCAPS to Detect CRISPR/Cas9 Induced Mutations
Bio-Protocol, 2019 Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment.
Charles Hodgens +2 more
doaj +1 more source
Site-specific restriction endonucleases in Bacillus licheniformis [PDF]
FEMS Microbiology Letters, 1995 We systematically studied site-specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A ...
C. PARINI, M. FORTINA
openaire +2 more sources
Methylation Sensitivity of Restriction Enzymes Interacting with GATC Sites [PDF]
BioTechniques, 2003 Albert Jeltsch, Andrea Hermann
openaire +5 more sources
Extraocular Muscle Imbalance and Outcomes of Scleral Buckling Surgery for Primary Rhegmatogenous Retinal Detachment [PDF]
, 2020 Objective: The objective was to study the muscle imbalance, restrictive motility in unlike gazes and the outcomes of the scleral buckling surgery for rhegmatogenous retinal detachment.
Kawish, A. B. (Ayesha) +2 more
core +1 more source
Chinese Journal of Lung Cancer, 2014 Background and objective The methods for introducing point mutations into target genes are important for dissecting the relationship of gene structure and function. Up to date, there are numbers of protocols available for the purpose.
Fanrong MENG +4 more
doaj +1 more source
Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
Scientific Reports, 2021 Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting ...
Federico Colombo +3 more
doaj +1 more source
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
doaj +1 more source
A one pot, one step, precision cloning method with high throughput capability.
PLoS ONE, 2008 Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed ...
Carola Engler +2 more
doaj +1 more source