Results 31 to 40 of about 1,167,532 (299)
Heliyon, 2019 Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova +3 more
doaj +1 more source
Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex [PDF]
, 2020 Background: Chromosomal inversion polymorphisms play a role in adaptation to heterogeneous environments. Inversion polymorphisms are implicated in the very high ecological flexibility of the three main malaria vector species of the Afrotropical Anopheles
Besansky, N. J. +10 more
core +1 more source
Combinatoric Properties of Restriction Sites [PDF]
The First International Conference on Symmetry, 2018 Endonucleases from the restriction–modification complex are known to recognise specific sites with tremendous precision. [...]
Elizaveta Taranenko, Mikhail Sadovsky
openaire +1 more source
A one pot, one step, precision cloning method with high throughput capability.
PLoS ONE, 2008 Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed ...
Carola Engler +2 more
doaj +1 more source
Rapid Generation of Nested Deletions by Differential Restriction Digestion
BioTechniques, 2002 A method was devised for generating nested deletions in DNA that exploits the difference in frequency of restriction sites recognized by compatible restriction endonucleases.
Jonathan J. Dennis, Gerben J. Zylstra
doaj +1 more source
Site-specific restriction endonucleases in Bacillus licheniformis [PDF]
FEMS Microbiology Letters, 1995 We systematically studied site-specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A ...
C. PARINI, M. FORTINA
openaire +1 more source
Frequencies of restriction sites
Nucleic Acids Research, 1983 Restriction sites or other sequence patterns are usually assumed to occur according to a Poisson distribution with mean equal to the reciprocal of the probability of the given site or pattern. For situations where non-overlapping occurrences of patterns, such as restriction sites, are the objects of interest, this note shows that the Poisson assumption
openaire +3 more sources
Restriction Site-Free Insertion of PCR Products Directionally into Vectors
BioTechniques, 2000 A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions.
G.J. Chen +4 more
doaj +1 more source
Performance and diarrhoea in piglets following weaning at seven weeks of age: Challenge with E. coli O 149 and effect of dietary factors [PDF]
, 2009 Four dietary factors (ad libitum versus feed restriction, control versus protein restriction at ad libitum feeding, control versus inclusion of lupine as a protein source at ad libitum feeding, and control versus extra vitamin E at ad libitum feeding ...
Højsgaard, Søren +4 more
core +1 more source
Automatic construction of restriction site maps [PDF]
Nucleic Acids Research, 1982 A computer program is described which constructs maps of restriction endonuclease cleavage sites in DNA molecules, given only the fragment lengths. The program utilizes fragment length data from single and double restriction enzyme digests to generate maps for linear or circular molecules.
openaire +2 more sources