DNA Research, 2016 Double-digest restriction site-associated DNA sequencing (ddRAD-Seq) enables high-throughput genome-wide genotyping with next-generation sequencing technology. Consequently, this method has become popular in plant genetics and breeding.
K. Shirasawa, H. Hirakawa, S. Isobe
semanticscholar +1 more source
Development of a molecular method for the rapid screening and identification of the three functionally relevant polymorphisms in the human TAS2R38 receptor gene in studies of sensitivity to the bitter taste of PROP [PDF]
, 2015 The objective of this work was to develop a rapid screening method to identify the three single nucleotide polymorphisms (SNPs) in the TAS2R38 gene, with the aim of providing a significant contribution to studies designed to assess sensitivity to the ...
Atzori, E, Orrù, R, Padiglia, A
core +2 more sources
Chimeric Gene Construction without Reference to Restriction Sites [PDF]
BioTechniques, 2002 Toshiro Shigaki, Kendal D. Hirschi
openalex +5 more sources
Sequence Capture versus Restriction Site Associated DNA Sequencing for Shallow Systematics. [PDF]
Systematic Biology, 2013 Sequence capture and restriction site associated DNA sequencing (RAD-Seq) are two genomic enrichment strategies for applying next-generation sequencing technologies to systematics studies.
Michael G. Harvey +4 more
semanticscholar +1 more source
Universal Restriction Site-Free Cloning Method Using Chimeric Primers
BioTechniques, 2002 A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector.
Guo Jun Chen +2 more
doaj +1 more source
REMP software to introduce a screening REstriction site in site-directed Mutagenesis Primer
SoftwareX, 2021 Insertion of silent mutations allowing for restriction site modification aids in the screening of successful mutants during site directed mutagenesis. Introducing a new restriction site requires the analysis of degenerate sequences within mutant primer ...
Madhavi Latha Yadav Bangaru, PhD +3 more
doaj
Using indCAPS to Detect CRISPR/Cas9 Induced Mutations
Bio-Protocol, 2019 Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment.
Charles Hodgens +2 more
doaj +1 more source
Systematic Biology, 2016 Restriction-site associated DNA sequencing (RAD-seq) and target capture of specific genomic regions, such as ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reduced-representation genomic data sets.
J. Manthey +3 more
semanticscholar +1 more source
Site-specific restriction endonucleases in Bacillus licheniformis [PDF]
FEMS Microbiology Letters, 1995 We systematically studied site-specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A ...
C. PARINI, M. FORTINA
openaire +2 more sources
Differentiation between Aspergillus flavus and Aspergillus parasiticus from Pure Culture and Aflatoxin-Contaminated Grapes Using PCR-RFLP Analysis of aflR-aflJ Intergenic Spacer [PDF]
, 2011 Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
Atoui, Ali +5 more
core +1 more source