Results 31 to 40 of about 1,179,472 (345)

Universal Restriction Site-Free Cloning Method Using Chimeric Primers

BioTechniques, 2002
A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector.
Guo Jun Chen, Nahong Qiu, Malcolm G.P. Page   +2 more
doaj   +1 more source

Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns [PDF]

, 1933
Hepatitis B virus (HBV) is classified into genotypes A–F, which is important for clinical and etiological investigations. To establish a simple genotyping method, 68 full-genomic sequences and 106 S gene sequences were analyzed by the molecular ...
Mizokami, Masashi, Nakano, Tatsunori, Orito, Etsuro, Tanaka, Yasuhito, Sakugawa, Hiroshi, Mukaide, Motokazu, Robertson, Betty H.   +6 more
core   +2 more sources

Extraocular Muscle Imbalance and Outcomes of Scleral Buckling Surgery for Primary Rhegmatogenous Retinal Detachment [PDF]

, 2020
Objective: The objective was to study the muscle imbalance, restrictive motility in unlike gazes and the outcomes of the scleral buckling surgery for rhegmatogenous retinal detachment.
Kawish, A. B. (Ayesha), Nawaz, S. (Sadia), Tahir, Q. (Qandeel)   +2 more
core   +1 more source

Using indCAPS to Detect CRISPR/Cas9 Induced Mutations

Bio-Protocol, 2019
Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment.
Charles Hodgens, Zachary Nimchuk, Joseph Kieber   +2 more
doaj   +1 more source

A Highly Efficient In Vitro Site-directed Mutagenesis Protocol for Introducing 
Multiple-site Mutations into Target Genes

Chinese Journal of Lung Cancer, 2014
Background and objective The methods for introducing point mutations into target genes are important for dissecting the relationship of gene structure and function. Up to date, there are numbers of protocols available for the purpose.
Fanrong MENG, Chen CHEN, Yongwen LI, Haisu WAN, Qinghua ZHOU   +4 more
doaj   +1 more source

Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution

Scientific Reports, 2021
Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting ...
Federico Colombo, Elisa Corsiero, Myles J. Lewis, Costantino Pitzalis   +3 more
doaj   +1 more source

An alternative approach to study the enzymatic specificities of the CfrBI restriction–modification system

Heliyon, 2019
Restriction–modification systems (RMS) are the main gene-engineering tools and a suitable model to study the molecular mechanisms of catalysis and DNA–protein interactions.
Marina V. Zakharova, Irina V. Beletskaya, Elena M. Ibryashkina, Alexander S. Solonin   +3 more
doaj   +1 more source

A one pot, one step, precision cloning method with high throughput capability.

PLoS ONE, 2008
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed ...
Carola Engler, Romy Kandzia, Sylvestre Marillonnet   +2 more
doaj   +1 more source

Combinatoric Properties of Restriction Sites [PDF]

The First International Conference on Symmetry, 2018
Endonucleases from the restriction–modification complex are known to recognise specific sites with tremendous precision. [...]
Elizaveta Taranenko, Mikhail Sadovsky
openaire   +1 more source

Rapid Generation of Nested Deletions by Differential Restriction Digestion

BioTechniques, 2002
A method was devised for generating nested deletions in DNA that exploits the difference in frequency of restriction sites recognized by compatible restriction endonucleases.
Jonathan J. Dennis, Gerben J. Zylstra
doaj   +1 more source