Results 41 to 50 of about 1,152,156 (344)
A simple method for DNA restriction site mapping [PDF]
Nucleic Acids Research, 1976 When a DNA molecule, enzymatically labelled with 32p at one end, is partially digested with a restriction enzyme labelled tdna fragments are obtained which form an overlapping series of molecules, all with a common labelled terminus. ta restriction map can then be constructed from an analysis of the size distribution of these molecules.
Hamilton O. Smith, Max L. Birnstiel
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Rapid Generation of Nested Deletions by Differential Restriction Digestion
BioTechniques, 2002 A method was devised for generating nested deletions in DNA that exploits the difference in frequency of restriction sites recognized by compatible restriction endonucleases.
Jonathan J. Dennis, Gerben J. Zylstra
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Frequencies of restriction sites
Nucleic Acids Research, 1983 Restriction sites or other sequence patterns are usually assumed to occur according to a Poisson distribution with mean equal to the reciprocal of the probability of the given site or pattern. For situations where non-overlapping occurrences of patterns, such as restriction sites, are the objects of interest, this note shows that the Poisson assumption
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Automatic construction of restriction site maps [PDF]
Nucleic Acids Research, 1982 A computer program is described which constructs maps of restriction endonuclease cleavage sites in DNA molecules, given only the fragment lengths. The program utilizes fragment length data from single and double restriction enzyme digests to generate maps for linear or circular molecules.
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Performance and diarrhoea in piglets following weaning at seven weeks of age: Challenge with E. coli O 149 and effect of dietary factors [PDF]
, 2009 Four dietary factors (ad libitum versus feed restriction, control versus protein restriction at ad libitum feeding, control versus inclusion of lupine as a protein source at ad libitum feeding, and control versus extra vitamin E at ad libitum feeding ...
Højsgaard, Søren +4 more
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Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat
Tropical Animal Science Journal, 2023 Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik +6 more
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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
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A strategy for seamless cloning of large DNA fragments from Streptomyces
BioTechniques, 2015 We report a novel method for the seamless cloning of large DNA fragments (SCLF) of up to 44 kb or larger from Streptomyces chromosomal DNA. SCLF is based on homologous recombination in Streptomyces and is easy to perform. The strategy of SCLF is to flank
Jun Huang +5 more
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Optimizing Restriction Site Placement for Synthetic Genomes
Information and Computation, 2010 AbstractRestriction enzymes are the workhorses of molecular biology. We introduce a new problem which arises in the course of our project to design virus variants to serve as potential vaccines: we wish to modify virus-length genomes to introduce large numbers of unique restriction enzyme recognition sites while preserving wild-type function by ...
Heraldo Memelli +5 more
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Network topology drives population temporal variability in experimental habitat networks
Population Ecology, EarlyView.Habitat patches connected by dispersal pathways form habitat networks. We explored how network topology affects population outcomes in laboratory experiments using a model species (Daphnia carinata). Central habitat nodes in complex lattice networks exhibited lower temporal variability in population sizes, suggesting they support more stable ...
Yiwen Xu +3 more
wiley +1 more source