Results 41 to 50 of about 1,179,472 (345)
Site-specific restriction endonucleases in Bacillus licheniformis [PDF]
FEMS Microbiology Letters, 1995 We systematically studied site-specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A ...
C. PARINI, M. FORTINA
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Restriction Site-Free Insertion of PCR Products Directionally into Vectors
BioTechniques, 2000 A restriction site-free cloning method has been developed for inserting a PCR product into a vector flexibly and precisely at any desired location with high efficiency. The method uses a pair of DNA integration primers with two portions.
G.J. Chen +4 more
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Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat
Tropical Animal Science Journal, 2023 Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik +6 more
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Frequencies of restriction sites
Nucleic Acids Research, 1983 Restriction sites or other sequence patterns are usually assumed to occur according to a Poisson distribution with mean equal to the reciprocal of the probability of the given site or pattern. For situations where non-overlapping occurrences of patterns, such as restriction sites, are the objects of interest, this note shows that the Poisson assumption
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Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
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Automatic construction of restriction site maps [PDF]
Nucleic Acids Research, 1982 A computer program is described which constructs maps of restriction endonuclease cleavage sites in DNA molecules, given only the fragment lengths. The program utilizes fragment length data from single and double restriction enzyme digests to generate maps for linear or circular molecules.
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Performance and diarrhoea in piglets following weaning at seven weeks of age: Challenge with E. coli O 149 and effect of dietary factors [PDF]
, 2009 Four dietary factors (ad libitum versus feed restriction, control versus protein restriction at ad libitum feeding, control versus inclusion of lupine as a protein source at ad libitum feeding, and control versus extra vitamin E at ad libitum feeding ...
Højsgaard, Søren +4 more
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A strategy for seamless cloning of large DNA fragments from Streptomyces
BioTechniques, 2015 We report a novel method for the seamless cloning of large DNA fragments (SCLF) of up to 44 kb or larger from Streptomyces chromosomal DNA. SCLF is based on homologous recombination in Streptomyces and is easy to perform. The strategy of SCLF is to flank
Jun Huang +5 more
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Frontiers in Marine Science, 2023 Montipora digitata is a species belonging to the Acroporidae. In the Indo-Pacific region, M. digitata is widely distributed and is the dominant species of scleractinian coral in the South China Sea, however, there are currently no molecular markers ...
Shuwen Jia +7 more
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Combinatorial Properties of Restriction Sites [PDF]
Journal of Siberian Federal University. Mathematics & Physics, 2018 Restriction sites are the strings in DNA recognised by their specific enzymes. Recognition of the sites is provided by their combinatoric pattern. This study presents some results obtained from 6-nucleotide long restriction sites analysis using duplet strength and editing distance ...
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