Results 41 to 50 of about 6,061,790 (368)

Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns [PDF]

, 1933
Hepatitis B virus (HBV) is classified into genotypes A–F, which is important for clinical and etiological investigations. To establish a simple genotyping method, 68 full-genomic sequences and 106 S gene sequences were analyzed by the molecular ...
Mizokami, Masashi, Nakano, Tatsunori, Orito, Etsuro, Tanaka, Yasuhito, Sakugawa, Hiroshi, Mukaide, Motokazu, Robertson, Betty H.   +6 more
core   +2 more sources

A Highly Efficient In Vitro Site-directed Mutagenesis Protocol for Introducing 
Multiple-site Mutations into Target Genes

Chinese Journal of Lung Cancer, 2014
Background and objective The methods for introducing point mutations into target genes are important for dissecting the relationship of gene structure and function. Up to date, there are numbers of protocols available for the purpose.
Fanrong MENG, Chen CHEN, Yongwen LI, Haisu WAN, Qinghua ZHOU   +4 more
doaj   +1 more source

Methylation Sensitivity of Restriction Enzymes Interacting with GATC Sites [PDF]

BioTechniques, 2003
Andrea Hermann, Albert Jeltsch
openalex   +5 more sources

Restriction Site-Associated DNA Sequencing (RAD-seq) Reveals an Extraordinary Number of Transitions among Gecko Sex-Determining Systems.

Molecular biology and evolution, 2015
Sex chromosomes have evolved many times in animals and studying these replicate evolutionary "experiments" can help broaden our understanding of the general forces driving the origin and evolution of sex chromosomes.
T. Gamble, J. Coryell, T. Ezaz, Joshua Lynch, D. P. Scantlebury, D. Zarkower   +5 more
semanticscholar   +1 more source

Phylogenomics of Phrynosomatid Lizards: Conflicting Signals from Sequence Capture versus Restriction Site Associated DNA Sequencing

Genome Biology and Evolution, 2015
Sequence capture and restriction site associated DNA sequencing (RADseq) are popular methods for obtaining large numbers of loci for phylogenetic analysis.
A. Leaché, Andreas S. Chavez, L. Jones, Jared A. Grummer, Andrew D. Gottscho, Charles W. Linkem   +5 more
semanticscholar   +1 more source

A SspI PCR-RFLP detecting a silent allele at the goat CSN2 locus [PDF]

, 2005
The comparison between the cDNA sequence obtained and the published sequences of the goat CSN2 alleles showed a new single nucleotide polymorphism (SNP) (transition C-T) at the 180th nucleotide of the ninth exon.
COSENZA, GIANFRANCO, DI BERARDINO D., GALLO, DANIELA, PAUCIULLO A., RAMUNNO L.   +4 more
core   +1 more source

Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution

Scientific Reports, 2021
Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting ...
Federico Colombo, Elisa Corsiero, Myles J. Lewis, Costantino Pitzalis   +3 more
doaj   +1 more source

Detection and Validation of QTL Affecting Bacterial Cold Water Disease Resistance in Rainbow Trout Using Restriction-Site Associated DNA Sequencing

PLoS ONE, 2015
Bacterial cold water disease (BCWD) causes significant economic loss in salmonid aquaculture. Using microsatellite markers in a genome scan, we previously detected significant and suggestive QTL affecting phenotypic variation in survival following ...
Y. Palti, R. Vallejo, Guangtu Gao, Sixin Liu, Alvaro G. Hernandez, C. Rexroad, G. Wiens   +6 more
semanticscholar   +1 more source

A one pot, one step, precision cloning method with high throughput capability.

PLoS ONE, 2008
Current cloning technologies based on site-specific recombination are efficient, simple to use, and flexible, but have the drawback of leaving recombination site sequences in the final construct, adding an extra 8 to 13 amino acids to the expressed ...
Carola Engler, Romy Kandzia, Sylvestre Marillonnet   +2 more
doaj   +1 more source

Rapid Generation of Nested Deletions by Differential Restriction Digestion

BioTechniques, 2002
A method was devised for generating nested deletions in DNA that exploits the difference in frequency of restriction sites recognized by compatible restriction endonucleases.
Jonathan J. Dennis, Gerben J. Zylstra
doaj   +1 more source