Results 51 to 60 of about 6,061,790 (368)
Frequencies of restriction sites
Nucleic Acids Research, 1983 Restriction sites or other sequence patterns are usually assumed to occur according to a Poisson distribution with mean equal to the reciprocal of the probability of the given site or pattern. For situations where non-overlapping occurrences of patterns, such as restriction sites, are the objects of interest, this note shows that the Poisson assumption
openaire +4 more sources
Automatic construction of restriction site maps [PDF]
Nucleic Acids Research, 1982 A computer program is described which constructs maps of restriction endonuclease cleavage sites in DNA molecules, given only the fragment lengths. The program utilizes fragment length data from single and double restriction enzyme digests to generate maps for linear or circular molecules.
openaire +3 more sources
Direct amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil [PDF]
, 2001 Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum
Adolphe Zeze +34 more
core +1 more source
Mutation Analysis and Restriction Site Mapping of GDF9 in Indonesian Bligon Goat
Tropical Animal Science Journal, 2023 Bligon goat is one of the crossbred goats raised in Indonesia, which has a prolific nature, potentially increasing the economic benefits. The gene that particularly influences prolific traits is Growth Differentiation Factor 9 (GDF9). This study aims to
T. Hartatik +6 more
doaj +1 more source
BMC Genomics, 2014 BackgroundCultivated peanut, or groundnut (Arachis hypogaea L.), is an important oilseed crop with an allotetraploid genome (AABB, 2n = 4x = 40). In recent years, many efforts have been made to construct linkage maps in cultivated peanut, but almost all ...
Xiaojing Zhou +9 more
semanticscholar +1 more source
G3: Genes, Genomes, Genetics, 2014 Recent advances in genotyping-by-sequencing have enabled genome-wide association studies in nonmodel species including those in aquaculture programs. As with other aquaculture species, rainbow trout and steelhead (Oncorhynchus mykiss) are susceptible to ...
N. Campbell +4 more
semanticscholar +1 more source
Mutation analysis using the restriction site mutation (RSM) assay [PDF]
, 1998 The restriction site mutation RSM assay see Steingrimsdottir et al. H. Steingrimsdottir, D. Beare, J. Cole, J.F.M. Leal, Ž. Ž w T. Kostic, J. Lopez-Barea, G. Dorado, A.R.
Hashemzadeh Chaleshtori, Morteza. +3 more
core +1 more source
A strategy for seamless cloning of large DNA fragments from Streptomyces
BioTechniques, 2015 We report a novel method for the seamless cloning of large DNA fragments (SCLF) of up to 44 kb or larger from Streptomyces chromosomal DNA. SCLF is based on homologous recombination in Streptomyces and is easy to perform. The strategy of SCLF is to flank
Jun Huang +5 more
doaj +1 more source
Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering
Fermentation, 2023 Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj +1 more source
Plasma Membrane-Associated Restriction Factors and Their Counteraction by HIV-1 Accessory Proteins. [PDF]
, 2019 The plasma membrane is a site of conflict between host defenses and many viruses. One aspect of this conflict is the host's attempt to eliminate infected cells using innate and adaptive cell-mediated immune mechanisms that recognize features of the ...
Guatelli, John +5 more
core +1 more source