Results 51 to 60 of about 1,152,156 (344)

A Method for Introducing Mutations into Large Vectors

Chinese Journal of Lung Cancer, 2014
Background and objective In vitro site-directed mutagenesis is a routine technique in molecular biology labs. However, although there are numbers of related methods available, most of these methods are not suitable for introducing mutations into large ...
Fanrong MENG, Chen CHEN, Haisu WAN, Qinghua ZHOU   +3 more
doaj   +1 more source

Site-directed mutagenesis of multi-copy-number plasmids: Red/ET recombination and unique restriction site elimination

BioTechniques, 2009
Existing methods for site-directed plasmid mutagenesis are restrained by the small spectrum of modifications that can be introduced by mutagenic primers and the amplicon size limitations of in vitro DNA synthesis.
Stephan Noll, Gabriele Hampp, Hanna Bausbacher, Natalia S. Pellegata, Harald Kranz   +4 more
doaj   +1 more source

Microsatellite markers for Monitipora digitata designed using restriction-site associated DNA sequencing

Frontiers in Marine Science, 2023
Montipora digitata is a species belonging to the Acroporidae. In the Indo-Pacific region, M. digitata is widely distributed and is the dominant species of scleractinian coral in the South China Sea, however, there are currently no molecular markers ...
Shuwen Jia, Yuanchao Li, Shiquan Chen, Zefu Cai, Jie Shen, Yi Wang, Zhongjie Wu, Daoru Wang   +7 more
doaj   +1 more source

Sandpiles with height restrictions

, 2002
We study stochastic sandpile models with a height restriction in one and two dimensions. A site can topple if it has a height of two, as in Manna's model, but, in contrast to previously studied sandpiles, here the height (or number of particles per site),
A. Chessa, A. Montakhab, A. Vespignani, A. Vespignani, A. Vespignani, A. Vespignani, C. Tang, D. Dhar, E. V. Ivashkevich, E. V. Ivashkevich, J. Kamphorst Leal da Silva, K. Binder, K. Binder, M. A. Muñoz, M. A. Muñoz, M. Rossi, M.E. Fisher, Mário J. de Oliveira, P. Bak, P. Bak, P. Grassberger, R. Dickman, R. Dickman, R. Dickman, R. Dickman, R. Dickman, Ronald Dickman, S. S. Manna, S. S. Manna, Tânia Tomé, V. B. Priezzhev   +30 more
core   +1 more source

Geographic variation in walking activity in the red flour beetle Tribolium castaneum

Population Ecology, EarlyView.
This study examined whether there is geographic variation in field populations, focusing on the moving activity in the red flour beetle Tribolium castaneum. Results showed significant differences in moving activity among field populations but no correlation with latitude or meteorological factors.
Kentarou Matsumura
wiley   +1 more source

occumb: An R package for site occupancy modeling of eDNA metabarcoding data

Population Ecology, EarlyView.
This study introduces a new R package, occumb, for the convenient application of site occupancy modeling using environmental DNA (eDNA) metabarcoding data. We outline a data analysis workflow, including data setup, model fitting, model assessment, and comparison of potential study settings based on model predictions, all of which can be performed using
Keiichi Fukaya, Yuta Hasebe
wiley   +1 more source

Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site

BMC Research Notes, 2017
Objective Human genomic libraries constructed in bacterial artificial chromosome vectors were utilized to make physical maps of all 23-chromosome pairs and as the templates for DNA sequencing to aid in the completion of the Human Genome Project. The goal
Jonathon S. Coren
doaj   +1 more source

Between Droughts and Floods: The Seasonal Response of Freshwater Snails in Artificial Reservoirs in the Brazilian Semiarid Region

Population Ecology, EarlyView.
We investigate the seasonal dynamics of two freshwater snails, Biomphalaria straminea and Melanoides tuberculata, in artificial reservoirs of the Brazilian semiarid region. Despite regulated hydrology, B. straminea exhibited strong seasonal fluctuations associated with dry periods, while M. tuberculata maintained stable populations throughout the year,
Lucas Henrique Sousa da Silva, João Hemerson de Sousa, Adriana Josefa da Rocha, Manuella Feitosa Leal, Lucas Ariel de Sousa Aguiar, Tamaris Gimenez Pinheiro, Edson Lourenço da Silva   +6 more
wiley   +1 more source

Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes [PDF]

, 2015
DNA cleavage by the Type III restriction enzymes requires long-range protein communication between recognition sites facilitated by thermally-driven 1D diffusion. This ‘DNA sliding’ is initiated by hydrolysis of multiple ATPs catalysed by a helicase-like
Bollins, Jack M, Szczelkun, Mark D, Tóth, Júlia   +2 more
core   +2 more sources

Moving in the Dark: Enlightening the Spatial Population Ecology of European Cave Salamanders

Population Ecology, EarlyView.
We assessed individual interactions, movement ecology and activity patterns of a subterranean population of Speleomantes strinatii, applying spatial capture–recapture modeling to a photographic dataset of 104 individuals. ABSTRACT Space use and movement are fundamental aspects of organisms' ecology, mirroring individual fitness, behavior, and life ...
Giacomo Rosa, Andrea Costa, Sebastiano Salvidio   +2 more
wiley   +1 more source