Results 61 to 70 of about 1,093,792 (283)
loxP-Directed Cloning: Use of Cre Recombinase as a Universal Restriction Enzyme
BioTechniques, 2001 We have developed a novel way to use the Cre/loxP system for in vitro manipulation of DNA and a technique to clone DNA into circular episomes. The method is fast, reliable, and allows flexible cloning of DNA fragments into episomes containing a loxP site.
Frank Buchholz, J. Michael Bishop
doaj +1 more source
Additional Acceleration of Protons and Energetic Neutrino Production in a Filamentary Jet of the Blazar Markarian 501 [PDF]
, 2009 Blazars have been regarded as one of the most powerful sources of the highest energy cosmic rays and also their byproducts, neutrinos. Provided that a magnetized filamentary system is established in a blazar jet as well, we could apply the mechanism of ...
Honda, M., Honda, Y. S.
core +1 more source
The power of microRNA regulation—insights into immunity and metabolism
FEBS Letters, EarlyView.MicroRNAs are emerging as crucial regulators at the intersection of metabolism and immunity. This review examines how miRNAs coordinate glucose and lipid metabolism while simultaneously modulating T‐cell development and immune responses. Moreover, it highlights how cutting‐edge artificial intelligence applications can identify miRNA biomarkers ...
Stefania Oliveto +2 more
wiley +1 more source
Restriction and modification of deoxyarchaeosine (dG+)-containing phage 9 g DNA
Scientific Reports, 2017 E. coli phage 9 g contains the modified base deoxyarchaeosine (dG+) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG+.
Rebecca Tsai +3 more
doaj +1 more source
Frequent occurrence of recognition Site-like sequences in the restriction endonucleases
BMC Bioinformatics, 2004 Background There are two different theories about the development of the genetic code. Woese suggested that it was developed in connection with the amino acid repertoire, while Crick argued that any connection between codons and amino acids is only the ...
Biro Jan C, Biro Josephine MK
doaj +1 more source
TALE-PvuII fusion proteins--novel tools for gene targeting. [PDF]
PLoS ONE, 2013 Zinc finger nucleases (ZFNs) consist of zinc fingers as DNA-binding module and the non-specific DNA-cleavage domain of the restriction endonuclease FokI as DNA-cleavage module.
Mert Yanik +5 more
doaj +1 more source
Identification of novel small molecule inhibitors of ETS transcription factors
FEBS Letters, EarlyView.ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla +9 more
wiley +1 more source
The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum
FEBS Letters, EarlyView.In Saccharomyces cerevisiae, Yeast Amino acid Transporter family members mediate the import of amino acids, ranging from substrate specialists to generalists. Here, we show that the specialist transporter, Lyp1, has a broader substrate spectrum than previously described, with affinity constants spanning from micromolar to millimolar.
Foteini Karapanagioti +3 more
wiley +1 more source
BMC Gastroenterology, 2012 Background Treatment effectiveness of Helicobacter pylori varies regionally and is decreasing worldwide, principally as a result of antibiotic resistant bacterium. Tetracycline is generally included in second line H. pylori eradication regimens.
Suzuki Rodrigo +2 more
doaj +1 more source
FEBS Letters, EarlyView.In lymphoid organs, antigen recognition and B cell receptor signaling rely on integrins and the cytoskeleton. Integrins act as mechanoreceptors, couple B cell receptor activation to cytoskeletal remodeling, and support immune synapse formation as well as antigen extraction.
Abhishek Pethe, Tanja Nicole Hartmann
wiley +1 more source