Inverse restriction site mutation (iRSM) analysis. Mutation detection involving the formation of restriction enzyme sites in target genes [PDF]
Mutagenesis, 1999 This paper describes a rapid screening procedure for the detection of DNA sequence changes resulting in the creation of new restriction enzyme sites. The basic methodology involves the identification of the conversion of one restriction site into another by mutagenesis.
Nobuo Takahashi +2 more
openaire +5 more sources
Insights into antibody catalysis: Structure of an oxygenation catalyst at 1.9-Å resolution [PDF]
, 1996 The x-ray crystal structures of the sulfide oxidase antibody 28B4 and of antibody 28B4 complexed with hapten have been solved at 2.2-Å and 1.9-Å resolution, respectively.
Hsieh-Wilson, Linda C. +2 more
core
Critical behavior of the two dimensional 2A->3A, 4A->0 binary system
, 2004 The phase transitions of the recently introduced 2A -> 3A, 4A -> 0 reaction-diffusion model (G.Odor, PRE 69 036112 (2004)) are explored in two dimensions. This model exhibits site occupation restriction and explicit diffusion of isolated particles.
G. Ódor +6 more
core +1 more source
A point mutation in the splice donor site of intron 7 in the as2-casein encoding gene of the Mediterranean River buffalo results in an allele-specific exon skipping [PDF]
, 2009 The CSN1S2 cDNA of 10 unrelated Mediterranean River Buffaloes reared in Southern Italy was amplified by RT-PCR, while the region from the 6th to the 8th exon of the CSN1S2 gene was amplified from genomic template.
COLETTA A. +6 more
core +1 more source
Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia
FEBS Letters, EarlyView.This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley +1 more source
BMC Gastroenterology, 2012 Background Treatment effectiveness of Helicobacter pylori varies regionally and is decreasing worldwide, principally as a result of antibiotic resistant bacterium. Tetracycline is generally included in second line H. pylori eradication regimens.
Suzuki Rodrigo +2 more
doaj +1 more source
Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]
, 1983 In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core
Spatial search in a honeycomb network
, 2010 The spatial search problem consists in minimizing the number of steps required to find a given site in a network, under the restriction that only oracle queries or translations to neighboring sites are allowed.
F. L. MARQUEZINO +5 more
core +1 more source
FEBS Letters, EarlyView.In lymphoid organs, antigen recognition and B cell receptor signaling rely on integrins and the cytoskeleton. Integrins act as mechanoreceptors, couple B cell receptor activation to cytoskeletal remodeling, and support immune synapse formation as well as antigen extraction.
Abhishek Pethe, Tanja Nicole Hartmann
wiley +1 more source
Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing
Chinese MedicineBackground Fritillariae Cirrhosae Bulbus is an antitussive and expectorant Chinese medicinal material derived from the dried bulbs of six Fritillaria species.
Hoi-Yan Wu +8 more
doaj +1 more source