Results 81 to 90 of about 1,093,792 (283)

insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments

BioTechniques, 2005
Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report
Ulrich Krauss, Thorsten Eggert
doaj   +1 more source

Whole genome phylogeny for 21 Drosophila species using predicted 2b-RAD fragments [PDF]

PeerJ, 2013
Type IIB restriction endonucleases are site-specific endonucleases that cut both strands of double-stranded DNA upstream and downstream of their recognition sequences.
Arun S. Seetharam, Gary W. Stuart
doaj   +2 more sources

The two-hole ground state of the Hubbard-Anderson model, approximated by a variational RVB-type wave function [PDF]

, 1994
In this paper the Hubbard-Anderson model on a square lattice with two holes is studied. The ground state (GS) is approximated by a variational RVB-type wave function. The holes interact by exchange of a localized spin excitation (SE), which is created or
Banning, E.J., Caspers, W.J., Traa, M.R.M.J.   +2 more
core   +2 more sources

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

Critical behavior of the two dimensional 2A->3A, 4A->0 binary system

, 2004
The phase transitions of the recently introduced 2A -> 3A, 4A -> 0 reaction-diffusion model (G.Odor, PRE 69 036112 (2004)) are explored in two dimensions. This model exhibits site occupation restriction and explicit diffusion of isolated particles.
G. Ódor, G. Ódor, Géza Ódor, I. Webman, J. Marro, M. Henkel, U. Täuber   +6 more
core   +1 more source

Decoding the dual role of autophagy in cancer through transcriptional and epigenetic regulation

FEBS Letters, EarlyView.
Transcriptional and epigenetic regulation controls autophagy, which exerts context‐dependent effects on cancer: Autophagy suppresses tumorigenesis by maintaining cellular homeostasis or promotes tumor progression by supporting survival under stress. In this “In a Nutshell” article, we explore the intricate mechanisms of the dual function of autophagy ...
Young Suk Yu, Ik Soo Kim, Sung Hee Baek
wiley   +1 more source

Restriction Enzyme-Mediated Integration Used to Produce Pathogenicity Mutants of Colletotrichum graminicola

Molecular Plant-Microbe Interactions, 2000
We have developed a restriction enzyme-mediated insertional mutagenesis (REMI) system for the maize pathogen Colletotrichum graminicola. In this report, we demonstrate the utility of a REMI-based mutagenesis approach to identify novel pathogenicity genes.
M. R. Thon, E. M. Nuckles, L. J. Vaillancourt   +2 more
doaj   +1 more source

Restriction of HIV-1 Genotypes in Breast Milk Does Not Account for the Population Transmission Genetic Bottleneck That Occurs following Transmission [PDF]

, 2010
BACKGROUND. Breast milk transmission of HIV-1 remains a major route of pediatric infection. Defining the characteristics of viral variants to which breastfeeding infants are exposed is important for understanding the genetic bottleneck that occurs in the
Aldrovandi, Grace M., Chen, Thomas, Conway, Susan, Decker, W. Don, Heath, Laura, Heil, Marintha, Hong, Jason, Jones, Laura, Kankasa, Chipepo, Kuhn, Louise, Mullins, James I., Nakamura, Kyle, Semrau, Katherine, Sinkala, Moses, Thea, Donald M., Walter, Jan   +15 more
core   +4 more sources