Results 81 to 90 of about 1,167,532 (299)

A simple method for DNA restriction site mapping [PDF]

open access: yesNucleic Acids Research, 1976
When a DNA molecule, enzymatically labelled with 32p at one end, is partially digested with a restriction enzyme labelled tdna fragments are obtained which form an overlapping series of molecules, all with a common labelled terminus. ta restriction map can then be constructed from an analysis of the size distribution of these molecules.
H O, Smith, M L, Birnstiel
openaire   +2 more sources

Mapping the evolution of mitochondrial complex I through structural variation

open access: yesFEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin   +2 more
wiley   +1 more source

BioNanoAnalyst: a visualisation tool to assess genome assembly quality using BioNano data

open access: yesBMC Bioinformatics, 2017
Background Reference genome assemblies are valuable, as they provide insights into gene content, genetic evolution and domestication. The higher the quality of a reference genome assembly the more accurate the downstream analysis will be. During the last
Yuxuan Yuan   +4 more
doaj   +1 more source

The phase transition of triplet reaction-diffusion models

open access: yes, 2006
The phase transitions classes of reaction-diffusion systems with multi-particle reactions is an open challenging problem. Large scale simulations are applied for the 3A -> 4A, 3A -> 2A and the 3A -> 4A, 3A->0 triplet reaction models with site occupation ...
G. Ódor   +3 more
core   +1 more source

Increased variability of microbial communities in restored salt marshes nearly 30 years after tidal flow restoration [PDF]

open access: yes, 2012
We analyzed microbial diversity and community composition from four salt marsh sites that were impounded for 40–50 years and subsequently restored and four unimpounded sites in southeastern Connecticut over one growing season.
Bernhard, Anne E   +2 more
core   +3 more sources

Ligation independent cloning irrespective of restriction site compatibility [PDF]

open access: yesNucleic Acids Research, 1997
Here we report the use of exonuclease to expose complementary DNA between an insert and vector such that annealing becomes independent of restriction site compatibility. We demonstrate that unusual and, in some cases, previously impossible cloning strategies can be readily and efficiently achieved as long as the flanking sequences of the linear vectors
C, Li, R M, Evans
openaire   +2 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

open access: yesFEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Restriction site heteroplasmy in the mitochondrial DNA of Brycon opalinus (Cuvier, 1819) (Characiformes, Characidae, Bryconiae)

open access: yesBrazilian Journal of Medical and Biological Research, 2004
Homoplasmy is a feature usually found in the mtDNA of higher animal taxa. On the other hand, the presence of two classes of mtDNA in the same cell or organism is rare and may appear in length or site variation.
A.W.S. Hilsdorf, J.E. Krieger
doaj   +1 more source

Spatial quantum search in a triangular network

open access: yes, 2010
The spatial search problem consists in minimizing the number of steps required to find a given site in a network, under the restriction that only oracle queries or translations to neighboring sites are allowed.
Ambainis   +7 more
core   +1 more source

Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving. [PDF]

open access: yes, 2011
Background The TaqII enzyme is a member of the Thermus sp. enzyme family that we propounded previously within Type IIS restriction endonucleases, containing related thermophilic bifunctional endonucleases-methyltransferases from various ...
Agnieszka Zylicz-Stachula   +4 more
core   +1 more source

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