Results 211 to 220 of about 7,318 (257)
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Hydrolysis of Retinyl Esters by Pancreatic Triglyceride Lipase
Biochemistry, 2000Previously [van Bennekum, A. M., et al. (1999) Biochemistry 38, 4150-4156] we showed that carboxyl ester lipase (CEL)-deficient (CELKO) mice have normal levels of pancreatic, bile salt-dependent retinyl ester hydrolase (REH) activity. In the present study, we further investigated this non-CEL REH activity in pancreas homogenates of CELKO and wild-type (
A M, van Bennekum +3 more
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Retinyl ester hydrolysis in the rabbit lacrimal gland
Current Eye Research, 1992The lacrimal gland stores retinyl esters which are synthesized by the enzyme acyl CoA:retinyl acyl transferase. Retinol is released from retinyl ester reserves by retinyl ester hydrolase (REH). Since the lacrimal gland secretes retinol, this gland should also contain this enzyme. To identify bile salt-dependent REH activity, rabbit lacrimal glands were
D L, Bernal, J L, Ubels
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Properties of liver retinyl ester hydrolase in young pigs
Biochimica et Biophysica Acta (BBA) - General Subjects, 1986A new sensitive method for the assay of retinyl ester hydrolase in vitro was developed and applied to liver homogenates of 18 young pigs with depleted-to-adequate liver vitamin A reserves. Radioactive substrate was not required, because the formation of retinol could be adequately quantitated by reversed-phase high-performance liquid chromatography ...
D A, Cooper, J A, Olson
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Nonaqueous electrochromatography on C30 columns: Separation of retinyl esters
Electrophoresis, 1999A nonaqueous packed capillary electrochromatographic reversed-phase method for separation of retinyl esters has been developed. The retinyl esters all-trans-retinyl acetate, palmitate, heptadecanoate, stearate, oleoate, and linoleoate were separated on a 180 microm ID column packed with 5 microm C30 particles with a mobile phase consisting of 2.5 mM ...
L, Roed, E, Lundanes, T, Greibrokk
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Retinyl and 3-dehydroretinyl esters in the crayfish retina
Vision Research, 1988We have studied chemical nature and localization of retinyl esters stored in the retina of the crayfish, Procambarus clarkii, which has a rhodopsin-porphyropsin visual pigment system. The crayfish kept at 10 degrees C in the constant dark had 3-dehydroretinal along with retinal in the retina as the chromophore of visual pigment.
T, Suzuki, Y, Maeda, Y, Toh, E, Eguchi
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In Vitro transfer of chylomicron retinol and retinyl esters
Biochemical and Biophysical Research Communications, 1983The redistribution of rat chylomicron retinoids following incubation with fasting- or postheparin human plasma was investigated. With fasting plasma, chylomicron retinol appeared among higher density lipoprotein acceptors and density greater than 1.21 gm/ml plasma proteins; only small amounts of retinyl ester were found therein. With postheparin plasma,
D E, Wilson, I F, Chan
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The Reversed-Phase HPLC Behavior of Retinyl Esters
Journal of Liquid Chromatography, 1989Abstract Fifteen retinyl esters were synthesized, including a number of esters containing naturally occurring polyunsaturated fatty acids. The esters were purified by HPLC and their chromatographic behavior on reversed phase HPLC was studied. A plot of Log k' versus % water in the eluting solvent for each ester was very close to linear in the range ...
Hubert E. May, Sung I. Koo
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HEPATIC STORES OF RETINOL AND RETINYL ESTERS IN ELDERLY PEOPLE
Age and Ageing, 1988Post-mortem concentrations of hepatic retinol and retinyl esters were determined in 40 subjects aged over 65 years to assess the effects of disease and malnutrition on vitamin A reserves. Three groups of patients (mean age 79.6 years) were studied: (1) previously healthy, (2) chronically ill, (3) chronically ill and wasted.
D A, Black, E, Heduan, D, Mitchell
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Retinol and retinyl esters in patients with alcoholic liver disease
Journal of Hepatology, 1989Liver retinoid levels and the retinyl esters were examined in liver biopsy specimens from 70 patients with alcoholic and nonalcoholic liver diseases. There was a wide variation in the liver retinoid levels. The liver retinoid level was statistically significantly lower in 15 patients with alcoholic liver disease and a depressed Normotest (NT) value of ...
H, Bell +5 more
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Hydrolysis of cis and trans isomers of retinyl palmitate by retinyl ester hydrolase of pig liver
Archives of Biochemistry and Biophysics, 1988Relative retinyl ester hydrolase activities of pig liver homogenates (n = 4) toward 9,13-cis-, 13-cis-, 9-cis-, and all-trans-retinyl palmitate were 6.8 +/- 0.5 (SE), 5.7 +/- 0.5, 2.4 +/- 0.1, and 1, respectively. The range of apparent Km values for the four isomers was 142 to 268 microM, and the pH optima were 8-9 in all cases.
D A, Cooper, J A, Olson
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