Results 91 to 100 of about 1,001,360 (298)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Vesicle reuse revisited [PDF]

Proceedings of the National Academy of Sciences, 2005
Neurons communicate at the synapse by releasing neurotransmitter from synaptic vesicles. After exocytosis, synaptic vesicle membrane and protein are locally recycled for reuse in the presynaptic terminal. The mechanisms involved in the recycling of synaptic vesicle material have yet to be fully resolved (reviewed in ref. 1).
openaire   +2 more sources

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Dynamic hydroxyl cycle of zeolite for short and ultra-short chain PFAS free potable water

Nature Communications
The global shift in per- and polyfluoroalkyl substance (PFAS) crisis towards (ultra-)short-chain congeners highlights the urgent need to design adsorbents with hydrophilic functions.
Yuanji Shi, Minghao Yang, Hongxin Mu, Haidong Hu, Hongqiang Ren   +4 more
doaj   +1 more source

Building Reusable Software Component For Optimization Check in ABAP Coding

, 2010
Software component reuse is the software engineering practice of developing new software products from existing components. A reuse library or component reuse repository organizes stores and manages reusable components.
Sharma, S. S. V. N., Shireesha, P.
core   +1 more source

Intercompartmental communication in senescence

FEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz, Eleanor J. Wind, Jixiang Leng, Myriam Gorospe   +3 more
wiley   +1 more source

Aberdeen University Ontology Reuse Stack [PDF]

, 2008
Postprin
Lam, Sik Chun, Pan, Jeff Z, Reul, Quentin Hugues, Sleeman, Derek, Thomas, Edward James   +4 more
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

In‐Depth Profiling Highlights the Effect of Efgartigimod on Peripheral Innate and Adaptive Immune Cells in Myasthenia Gravis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Myasthenia gravis (MG) is an autoimmune disorder characterized by antibody‐mediated complement activation. Efgartigimod, a neonatal Fc receptor (FcRn) antagonist, is approved for treating generalized MG (gMG). However, its modulatory effects on upstream innate and adaptive immune cells remain largely unexplored.
Lei Jin, Liu Dong, Ying Wang, Dingxian He, Chong Yan, Jianying Xi, Haoqin Jiang, Yu Shi, Ming Guan, Chongbo Zhao, Jie Song, Sushan Luo   +11 more
wiley   +1 more source

Synergistic iron enhanced aerogel and peracetic acid for degradation of emerging organic contaminants

npj Clean Water
In response to the urgent need for efficient degradation of emerging organic contaminants, this study has developed a novel catalytic system based on an original Fe-doped aerogel catalyst (FeCAS) and its carbonization-enhanced variant (FeCAS-400 ...
Lili Jin, Tong Li, Xiaoya Fang, Zhao Xue, Hui Huang, Hongqiang Ren   +5 more
doaj   +1 more source