Results 261 to 270 of about 171,614 (301)

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

2003
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
openaire   +2 more sources

Genome‐controlled reverse transcriptase‐polymerase chain reaction for targeted gene‐expression analysis

Scandinavian Journal of Clinical and Laboratory Investigation, 2006
Although gene-expression profiling has an important part to play in the classification of tumours and premalignant conditions, reproducibility of the present polymerase chain reaction (PCR)-based quantitative techniques needs to be improved for diagnostic purposes and to enable analysis of gene expression in formalin-fixed paraffin-embedded (FFPE ...
J, Stenman, J, Räsänen, T, Tenkanen, C, Haglund, J, Salo, A, Orpana, A, Paju   +6 more
openaire   +2 more sources

Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction

Avian Diseases, 1997
A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven ...
Z, Xie, A A, Fadl, T, Girshick, M I, Khan   +3 more
openaire   +2 more sources

A Reverse Transcriptase-Polymerase Chain Reaction Assay for Detecting Highlands J Virus

Avian Diseases, 2001
Highlands J (HJ) virus is an arbovirus frequently recovered at high rates in mosquitoes collected in the eastern United States. HJ virus is primarily a veterinary pathogen causing disease in domestic birds including turkeys, chickens, and partridges.
Whitehouse, C. A., Guibeau, A., McGuire, D., Takeda, T., Mather, T. N.   +4 more
openaire   +3 more sources

Quantitative Reverse Transcriptase Polymerase Chain Reaction Assay for Mouse Androgen Receptor mRNA

Endocrine, 2001
A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) assay for mouse androgen receptor (AR) mRNA was developed to study relative changes in AR gene expression. Serial dilutions of a standard comprising a fragment of the ampicillin resistance gene flanked by the primer sequences of the AR mRNA were added to a constant amount of total ...
G J, Foxley, Q, Dong, D J, Handelsman
openaire   +2 more sources

Prognostic Value of Tyrosinase Reverse-Transcriptase Polymerase Chain Reaction in Metastatic Melanoma

Dermatology, 2001
<i>Background:</i> Circulating tumor cells can now be identified in subjects with metastatic melanoma by using the reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the messenger RNA of tyrosinase, a key enzyme of melanogenesis. <i>Aim and Methods:</i> The aim of this study was to determine whether the detection
G, Quereux, M, Denis, A, Khammari, P, Lustenberger, B, Dreno   +4 more
openaire   +2 more sources

Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction

Pathology, 2000
A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
P C, McMinn, P G, Carman, D W, Smith
openaire   +2 more sources

Application of Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Urological Oncology

Journal of Urology, 2003
During the last decade numerous different reverse transcriptase-polymerase chain reaction (RT-PCR) techniques have been described. However, the lack of highly sensitive, quantitative and reliable methodology has been responsible for its limited use in modern urology.
Andres Jan, Schrader, Joerg, Lauber, Oskar, Lechner, Axel, Heidenreich, Rainer, Hofmann, Jan, Buer   +5 more
openaire   +2 more sources

Reverse transcriptase-polymerase chain reaction assay for Acetylcholinesterase mRNA in rat brain

Neurochemical Research, 1995
In order to examine the molecular basis for regional variation in expression of brain acetylcholinesterase (AChE), an assay using reverse transcription and polymerase chain reaction (RT-PCR) was developed to measure steady state levels of AChE mRNA. The amplification method was designed to be specific for templates derived from AChE mRNA and to avoid ...
R, Rao, S, Brimijoin
openaire   +2 more sources

Analysis of Gene Expression by Reverse Transcriptase-Polymerase Chain Reaction

1993
Abstract A molecular genetic approach to development often involves isolating genes controlling cellular developmental processes, analysing their structure and regulation, and gaining clues about their function by examining their expression.
openaire   +1 more source