Results 301 to 310 of about 275,235 (344)

Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen mRNA

Clinical Biochemistry, 1997
To develop a quantitative reverse transcriptase-polymerase chain reaction assay for monitoring the prostate-specific antigen (PSA) mRNA.PSA mRNA is amplified, in parallel, with the mRNA of beta-actin, a housekeeping gene. The ratio of the amplification products obtained reflects the relative amount of PSA mRNA with respect to actin mRNA.
B, Galvan, T K, Christopoulos
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Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis

Archives of Otolaryngology - Head and Neck Surgery, 1996
To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
M M, April, J M, Garelick, G J, Nuovo
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Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Molecular and Biochemical Parasitology, 2000
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
M, Menegon, C, Severini, A, Sannella, M G, Paglia, D, Sangaré, A, Abdel-Wahab, A A, Abdel-Muhsin, H, Babiker, D, Walliker, P, Alano   +9 more
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Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)

2003
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
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Genome‐controlled reverse transcriptase‐polymerase chain reaction for targeted gene‐expression analysis

Scandinavian Journal of Clinical and Laboratory Investigation, 2006
Although gene-expression profiling has an important part to play in the classification of tumours and premalignant conditions, reproducibility of the present polymerase chain reaction (PCR)-based quantitative techniques needs to be improved for diagnostic purposes and to enable analysis of gene expression in formalin-fixed paraffin-embedded (FFPE ...
J, Stenman, J, Räsänen, T, Tenkanen, C, Haglund, J, Salo, A, Orpana, A, Paju   +6 more
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Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction

Avian Diseases, 1997
A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven ...
Z, Xie, A A, Fadl, T, Girshick, M I, Khan   +3 more
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A Reverse Transcriptase-Polymerase Chain Reaction Assay for Detecting Highlands J Virus

Avian Diseases, 2001
Highlands J (HJ) virus is an arbovirus frequently recovered at high rates in mosquitoes collected in the eastern United States. HJ virus is primarily a veterinary pathogen causing disease in domestic birds including turkeys, chickens, and partridges.
Whitehouse, C. A., Guibeau, A., McGuire, D., Takeda, T., Mather, T. N.   +4 more
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Quantitative Reverse Transcriptase Polymerase Chain Reaction Assay for Mouse Androgen Receptor mRNA

Endocrine, 2001
A quantitative reverse transcriptase polymerase chain reaction (RT-PCR) assay for mouse androgen receptor (AR) mRNA was developed to study relative changes in AR gene expression. Serial dilutions of a standard comprising a fragment of the ampicillin resistance gene flanked by the primer sequences of the AR mRNA were added to a constant amount of total ...
G J, Foxley, Q, Dong, D J, Handelsman
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Prognostic Value of Tyrosinase Reverse-Transcriptase Polymerase Chain Reaction in Metastatic Melanoma

Dermatology, 2001
<i>Background:</i> Circulating tumor cells can now be identified in subjects with metastatic melanoma by using the reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the messenger RNA of tyrosinase, a key enzyme of melanogenesis. <i>Aim and Methods:</i> The aim of this study was to determine whether the detection
G, Quereux, M, Denis, A, Khammari, P, Lustenberger, B, Dreno   +4 more
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Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction

Pathology, 2000
A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
P C, McMinn, P G, Carman, D W, Smith
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