Results 301 to 310 of about 273,066 (341)
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Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction
Molecular and Biochemical Parasitology, 2000A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
M, Menegon +9 more
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Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)
2003Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene ...
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Canadian Journal of Microbiology, 1998
Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Y L, Tsai, S L, Parker
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Reverse transcriptase - polymerase chain reaction (RT-PCR) has been used extensively to detect enteric viruses in environmental samples. Advantages of RT-PCR include its high detection sensitivity and rapid turn-around time. However, unlike traditional cell culture, RT-PCR has not provided quantitation and infectivity information.
Y L, Tsai, S L, Parker
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Nature Biotechnology, 1993
Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants.
J M, Irving, L W, Chang, F J, Castillo
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Specific hybridization primers for the PCR assay were developed to detect the presence of the ecotropic, xenotropic, and mink cell focus-forming classes of murine leukemia viruses (MuLVs) in samples derived from cultured cells and cell-free supernatants.
J M, Irving, L W, Chang, F J, Castillo
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Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction
2003In oncology, the correct assessment of tumor stage is crucial information, as it relates to disease-free interval and prognosis. Routine staging modalities use histopathology to evaluate the presence or absence of metastasis mainly in tissues such as lymph nodes, as described in Chapter 1 by Roskell and Buley.
K, Haack, K, Soondrum, M, Neumaier
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Journal of Orthopaedic Science, 2004
The purpose of our study was to assess microinvasion in a case of synovial sarcoma by means of the reverse transcriptase polymerase chain reaction (RT-PCR) for detecting SYT-SSX fusion transcripts. Furthermore, we tried to compare the RT-PCR results with those obtained by conventional histopathological examination.
Jun, Nakasone +6 more
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The purpose of our study was to assess microinvasion in a case of synovial sarcoma by means of the reverse transcriptase polymerase chain reaction (RT-PCR) for detecting SYT-SSX fusion transcripts. Furthermore, we tried to compare the RT-PCR results with those obtained by conventional histopathological examination.
Jun, Nakasone +6 more
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Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction
Avian Diseases, 1997A reverse transcriptase-polymerase chain reaction method was developed for the detection of avian reovirus. The origin of primers was from the S1 gene of the avian reovirus genome. A reovirus-specific 532-base pair cDNA product was amplified by these primers from six reference strains and 23 field isolates of avian reoviruses, but not from seven ...
Z, Xie +3 more
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Detection and Identification of Flaviviruses by Reverse Transcriptase Polymerase Chain Reaction
1992We developed a reverse transcriptase/polymerase chain reaction (RT/PCR) for the detection and identification of flavivirus RNA in a single-tube assay. Universal flavivirus RT/PCR amplimers amplify an 832-bp region in the carboxyl portion of NS5 (nt9166-9997) which contains nucleotide sequences conserved among all flaviviruses at the 3′- and 5 ...
Dennis W. Trent, Gwong-Jen Chang
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Dermatology, 2001
<i>Background:</i> Circulating tumor cells can now be identified in subjects with metastatic melanoma by using the reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the messenger RNA of tyrosinase, a key enzyme of melanogenesis. <i>Aim and Methods:</i> The aim of this study was to determine whether the detection
G, Quereux +4 more
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<i>Background:</i> Circulating tumor cells can now be identified in subjects with metastatic melanoma by using the reverse-transcriptase polymerase chain reaction (RT-PCR) to detect the messenger RNA of tyrosinase, a key enzyme of melanogenesis. <i>Aim and Methods:</i> The aim of this study was to determine whether the detection
G, Quereux +4 more
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Early diagnosis of murray valley encephalitis by reverse transcriptase-polymerase chain reaction
Pathology, 2000A 4-year-old aboriginal boy developed encephalitis due to Murray Valley encephalitis virus (MVE) following an earlier infection with Kunjin virus (KUN). The illness was severe, resulting in cerebral atrophy and profound physical and intellectual disability.
P C, McMinn, P G, Carman, D W, Smith
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