Results 301 to 310 of about 266,159 (347)

Impaired angiogenesis in gestational diabetes is linked to succinate/SUCNR1 axis dysregulation in late gestation

The Journal of Physiology, EarlyView.
Abstract figure legend Gestational Diabetes Mellitus (GDM) impacts on succinate metabolism and angiogenesis in the umbilical cord endothelium. (Left Panel) Succinate levels increase in both maternal and fetal circulation at delivery, with higher concentrations observed in GDM pregnancies compared to controls during late gestation.
Sergiy Klid, Francisco Algaba‐Chueca, Elsa Maymó‐Masip, Mónica Ballesteros, Montse Inglés, Albert Guarque, Nerea Vilanova‐Ricart, Ariadna Prats, Azra Kulovic‐Sissawo, Elisa Weiss, Ursula Hiden, Joan Vendrell, Sonia Fernández‐Veledo, Ana Megía   +13 more
wiley   +1 more source

Bluetongue diagnosis by reverse transcriptase-polymerase chain reaction

, 2003
Stéphan Zientara, Emmanuel Bréard, Corinne Sailleau   +2 more
openalex   +1 more source

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Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction

Journal of Medical Virology, 1991
AbstractRNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN‐2), and dengue 4 (DEN‐4)
Mark S. Godec, C. J. Gibbs, Zayd A. Eldadah, Kitty L. Pomeroy, Stephen M. Feinstone, David M. Asher, Herbert Levitan, D. Carleton Gajdusek, Lev G. Goldfarb   +8 more
openaire   +3 more sources

Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses

Journal of Veterinary Medicine, Series B, 2002
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
Helmut Hotzel, Konrad Sachse, Peter Otto, Jutta Prudlo, Mandy C. Elschner   +4 more
openaire   +2 more sources

Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus

Avian Diseases, 2005
A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome.
Theodore Girshick, Xie Zhixun, Xie Zhiqin, Mazhar I. Khan   +3 more
openaire   +3 more sources

Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis

Archives of Otolaryngology - Head and Neck Surgery, 1996
To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
Max M. April, Jordan M. Garelick, Gerard J. Nuovo   +2 more
openaire   +3 more sources

Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Molecular and Biochemical Parasitology, 2000
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
Carlo Severini, Abdel Muhsin A Abdel-Muhsin, Pietro Alano, Djibril Sangaré, David Walliker, Amel Abdel-Wahab, Maria Grazia Paglia, Hamza A. Babiker, Anna Rosa Sannella, Michela Menegon   +9 more
openaire   +3 more sources

Detection of measles virus by reverse-transcriptase polymerase chain reaction in a placenta

The Journal of Maternal-Fetal & Neonatal Medicine, 2009
Measles virus (MV) during pregnancy is associated with maternal morbidity and mortality and can put the fetus and newborn at risk of a wide range of complications. Reverse-transcriptase polymerase chain reaction (RT-PCR) for detecting MV in the placenta has not been reported.A case of RT-PCR detection of MV in the placenta of a 38-year-old woman who ...
Ariel Many, Yair Aboudy, Ora Halutz, Shikma Bar-On, Yifat Ochshorn   +4 more
openaire   +3 more sources

Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Ryan S. Robetorye, Hongxin Fan
openaire   +3 more sources

Prognostic Value of Circulating Melanoma Cells Detected by Reverse Transcriptase–Polymerase Chain Reaction

Journal of Clinical Oncology, 2003
Purpose: Factors that are predictive of prognosis in patients who are diagnosed with malignant melanoma (MM) are widely awaited. Detection of circulating melanoma cells (CMCs) by reverse transcriptase-polymerase chain reaction (RT-PCR) has recently been postulated as a possible negative prognostic factor.
PALMIERI G, ASCIERTO PA, PERRONE F, SATRIANO SMR, OTTAIANO A, DAPONTE A, NAPOLITANO M, CARACÒ C, MOZZILLO N, MELUCCI MT, COSSU A, TANDA F, GALLO, Ciro, SATRIANO RA, CASTELLO G.   +14 more
openaire   +4 more sources