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Detection of flaviviruses by reverse‐transcriptase polymerase chain reaction

Journal of Medical Virology, 1991
AbstractRNA sequences of five flaviviruses were detected by a modified polymerase chain reaction (PCR) that incorporated a reverse transcriptase and RNase inhibitor. Oligonucleotide primer pairs were synthesized to amplify sequences from St. Louis encephalitis (SLE), Japanese encephalitis (JBE), yellow fever (YF), dengue 2 (DEN‐2), and dengue 4 (DEN‐4)
Mark S. Godec, C. J. Gibbs, Zayd A. Eldadah, Kitty L. Pomeroy, Stephen M. Feinstone, David M. Asher, Herbert Levitan, D. Carleton Gajdusek, Lev G. Goldfarb   +8 more
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Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.

Analytical Biochemistry, 1999
In this article we present validation of a real-time RT-PCR method to quantitate mRNA expression levels of atrial natriuretic peptide and c-fos in an in vitro model of cardiac hypertrophy.
J. Winer, Christopher Kwang S. Jung, Irene Shackel, P. Williams   +3 more
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Nested Reverse Transcriptase‐Polymerase Chain Reaction for the Detection of Group A Rotaviruses

Journal of Veterinary Medicine, Series B, 2002
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the detection of group A rotaviruses was developed, which is based on a target region in gene segment 6. Rotavirus strains of human, bovine, porcine, canine,
Helmut Hotzel, Konrad Sachse, Peter Otto, Jutta Prudlo, Mandy C. Elschner   +4 more
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Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus

Avian Diseases, 2005
A reverse transcriptase-polymerase chain reaction (RT-PCR) was developed and optimized for the detection of avian encephalomyelitis virus (AEV). A pair of primers was prepared based on the VP2 gene of the structural protein P1 region of the AEV genome.
Theodore Girshick, Xie Zhixun, Xie Zhiqin, Mazhar I. Khan   +3 more
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Genotyping of Plasmodium falciparum gametocytes by reverse transcriptase polymerase chain reaction

Molecular and Biochemical Parasitology, 2000
A molecular assay has been developed for the specific detection and genetic characterisation of Plasmodium falciparum gametocytes in the blood of malaria infected individuals. The assay is based on the reverse transcription and polymerase chain reaction (RT-PCR) amplification of the messenger RNA of gene pfg377, a sexual-stage specific transcript ...
Carlo Severini, Abdel Muhsin A Abdel-Muhsin, Pietro Alano, Djibril Sangaré, David Walliker, Amel Abdel-Wahab, Maria Grazia Paglia, Hamza A. Babiker, Anna Rosa Sannella, Michela Menegon   +9 more
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Detection of measles virus by reverse-transcriptase polymerase chain reaction in a placenta

The Journal of Maternal-Fetal & Neonatal Medicine, 2009
Measles virus (MV) during pregnancy is associated with maternal morbidity and mortality and can put the fetus and newborn at risk of a wide range of complications. Reverse-transcriptase polymerase chain reaction (RT-PCR) for detecting MV in the placenta has not been reported.A case of RT-PCR detection of MV in the placenta of a 38-year-old woman who ...
Ariel Many, Yair Aboudy, Ora Halutz, Shikma Bar-On, Yifat Ochshorn   +4 more
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Reverse Transcriptase In Situ Polymerase Chain Reaction in Atypical Mycobacterial Adenitis

Archives of Otolaryngology - Head and Neck Surgery, 1996
To determine whether reverse transcriptase (RT) in situ polymerase chain reaction (PCR) can facilitate the diagnosis of nontuberculous ("atypical") mycobacterial (NTM) cervical adenitis.Retrospective review of 12 patients with neck masses clinically diagnosed as NTM cervical adenitis.University medical center caring for both ambulatory and hospitalized
Max M. April, Jordan M. Garelick, Gerard J. Nuovo   +2 more
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Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Ryan S. Robetorye, Hongxin Fan
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